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CAGGS-losSTOPlox-ClaI flp-in

Plasmid

#21548

Depositor

Rudolf Jaenisch

Article

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Insert

Use

Mouse Targeting

Expression

Mammalian

Available Since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pLX - T7pro - EBFP

Plasmid

#138615

Purpose

Express EBFP with an upstream T7 promoter in a lentiviral backbone

Depositor

Fei Chen

Article

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

EBFP

Use

Lentiviral

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF1a-LMO2-IRES-Puro

Plasmid

#61064

Purpose

lentiviral vector for LMO2

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

LMO2 (LMO2 Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1alpha

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-24p3/LCN2-Luc

Plasmid

#25463

Depositor

Sarah Gaffen

Article

Shen et al J Leukoc Biol. 2005 Mar . 77(3):388-99.

Insert

24p3/lipocalin 2 proximal promoter (Lcn2 Mouse)

Use

Luciferase

Mutation

none

Available Since

June 25, 2010

Availability

Academic Institutions and Nonprofits only
Pdx1 Cre-ER (DM#255)

Plasmid

#15022

Depositor

Douglas Melton

Article

Gu et al Development. 2002 May . 129(10):2447-57.

Insert

Pdx1 promoter (Pdx1 Mouse)

Use

Cre/Lox; Mouse transgenic

Tags

Cre-ER(TM)

Expression

Mammalian

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pJ3 SHP2 CS

Plasmid

#8319

Depositor

Ben Neel

Article

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Mutation

C459S, mutation of essential catalytic cysteine

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Ascl2

Plasmid

#71151

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ascl2/Mash2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Ascl2 (Ascl2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pLBS-mScarlet

Plasmid

#129337

Purpose

cytosol marker, lentivirus

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

mScarlet (Addgene 85042)

Use

Lentiviral

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pFF745

Plasmid

#61450

Purpose

IPTG-inducible SCRIBE_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_kanR(ON)

Use

Synthetic Biology

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-StrepHA-SUMO2

Plasmid

#66867

Purpose

Mammalian expression of SUMO2 with a strep and an HA tag

Depositor

Ying Liu

Article

Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

Insert

SUMO2 (SUMO2 Human)

Tags

HA and Strep

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Mirk (pHX9F)

Plasmid

#17846

Depositor

Eileen Friedman

Article

Lee et al Cancer Res. 2000 Jul 1. 60(13):3631-7.

Insert

Mirk (DYRK1B Human)

Tags

6xHis and Anti-XPress

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
L1-Fc

Plasmid

#15124

Depositor

Vance Lemmon

Article

Fransen et al Hum Mol Genet. 1998 Jun . 7(6):999-1009.

Insert

L1-Fc (L1CAM Human)

Expression

Mammalian

Mutation

Chimeric molecule consisting of the Fc region of …

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
pMB1609_pRR-EGFP

Plasmid

#65852

Purpose

EGFP-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

Depositor

Marc Bühler

Article

Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

Insert

split EGFP coding sequence

Use

CRISPR and TALEN

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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EF1a_CDX2_P2A_Hygro_Barcode

Plasmid

#120432

Purpose

Barcoded lentiviral vector to express CDX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

CDX2 (CDX2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry-Akt1(WT)

Plasmid

#86631

Purpose

Expresses mCherry-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDS277

Plasmid

#34973

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

V529N

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-Mma PylRS

Plasmid

#140009

Purpose

Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGEM-human TCR zeta/2470

Plasmid

#11507

Depositor

Allan Weissman

Article

Weissman et al Proc Natl Acad Sci U S A. 1988 Dec . 85(

Insert

TCR zeta (CD247 Human)

Use

General cloning

Mutation

n/a

Available Since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

Plasmid

#188375

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCaggs-mGold

Plasmid

#157996

Purpose

Expression of mGold in mammalian cells

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

mGold

Expression

Mammalian

Mutation

mGold is mVenus with L46F;T63S mutations

Promoter

Actin promoter with CMV enhancer

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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