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We narrowed to 10,121 results for: Cad

Showing: 5791 - 5820 of 10121 results

  3. pET24a-VHH-1xTS

    Plasmid
    #109418
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to one tyrosine sulfation (TS) motif. VHH-1xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109418-plasmid-map-sequence-id-211591

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  5. GST-CHMP4A (205-222) L217A (pGEX2T(TEV))

    Plasmid
    #80638
    Purpose
    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-204
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Residues 205-222; Changed Leucine 217 to Alanine
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80638-plasmid-map-sequence-id-161738

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  6. GST-CHMP4A (205-222) W220A (pGEX2T(TEV))

    Plasmid
    #80637
    Purpose
    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-203
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Residues 205-222; Changed Trp 220 to Ala
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80637-plasmid-map-sequence-id-161733

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  7. GST-CHMP4A (205-222) L214A (pGEX2T(TEV))

    Plasmid
    #80639
    Purpose
    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-205
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Residues 205-222; Changed Leucine 214 to Alanine
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80639-plasmid-map-sequence-id-161725

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  10. pET21b(+)-Is-PETase

    Plasmid
    #112202
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    Promoter
    N/A
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112202-plasmid-map-sequence-id-334619

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  12. pCJ136

    Plasmid
    #162665
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162665-plasmid-map-sequence-id-321451

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  15. pNon-target

    Plasmid
    #214053
    Purpose
    Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Non-target sequence
    Use
    Non-target rna plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214053-plasmid-map-sequence-id-428839

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  16. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid
    #50831
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    Use
    Baculoviral
    Tags
    His, Myc-6xHis, and WPRE
    Expression
    Insect
    Mutation
    Y39TAG and able to charge various unnatural amino…
    Promoter
    CAG, CMV, and U6 and H1
    Available since
    June 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    50831-plasmid-map-sequence-id-193569

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  18. pCH1/RAD51o

    Plasmid
    #102562
    Purpose
    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon
    Depositor
    Maria Spies
    Article
    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11
    Inserts
    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
    E. coli groE operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli Expression
    Promoter
    T7
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    102562-plasmid-map-sequence-id-203161

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  22. eGFP-proα1(I)

    Plasmid
    #119843
    Purpose
    Expresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α1 chain (Col1a1 Mouse)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Col1a1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119843-plasmid-map-sequence-id-240348

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  27. pLenti PGK mKate2-OGT K842M (Puro)

    Plasmid
    #154291
    Purpose
    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, K842M variant, catalytic dead)
    Depositor
    Suzanne Walker
    Article
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Lentiviral
    Tags
    2x FLAG and mKate2
    Expression
    Mammalian
    Mutation
    K842M mutation (Catalytic Inactive)
    Promoter
    PGK
    Available since
    Dec. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154291-plasmid-map-sequence-id-332432

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  29. eGFP-proα2(I)-L

    Plasmid
    #120160
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    120160-plasmid-map-sequence-id-233870

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Showing: 5791 - 5820 of 10121 results