We narrowed to 12,110 results for: NSI
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Plasmid#184058Purposecyclofen-inducible CRE activation via mammalian cell transfection or mRNA synthesisDepositorInsert6myc-CRE-ERT2
UseSynthetic BiologyTags6xMycExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available SinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_NPYmut
Plasmid#208678PurposeExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cellsDepositorInsertGPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
ExpressionMammalianPromoterCMVAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13-IDR-mCherry-CRY2
Plasmid#145258PurposeMammalian Expression of Opto HOXD13 IDRDepositorInsertHOXD13wt-IDR (HOXD13 Human)
ExpressionMammalianAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HSPA5P
Plasmid#44150Purposea fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)DepositorInsertHSPA5 promoter (HSPA5 Human)
ExpressionMammalianAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-321/+120
Plasmid#35540DepositorUseLuciferaseTagsluciferasePromoternoneAvailable SinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCFP-LacI-HOXD13wt-IDR
Plasmid#145255PurposeExpresses fusion of ECFP-LacI and HOXD13wt IDRDepositorInsertHOXD13wt-IDR (HOXD13 Human)
ExpressionMammalianAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-FLIP pr5
Plasmid#19130DepositorInsertFLIP promoter (CFLAR Human)
UseLuciferaseExpressionMammalianMutationThe sequence corresponds to positions -503 to +28…Available SinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-EGFP.T2A.NCLX
Plasmid#181873PurposeExpresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoterDepositorInsertsUseAAV and AdenoviralTagsHA and mycExpressionMammalianPromotersynthetic hybrid CAG promoter and synthetic hybri…Available SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_UCN1.0
Plasmid#208668PurposeExpresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in neuronsDepositorInsertGPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
UseAAVPromoterhSynAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLPC-FLAG.Mm.DMAP1
Plasmid#184650PurposeRetroviral expression of mouse DMAP1DepositorAvailable SinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-E2F1
Plasmid#37141DepositorInsertE2F1 (E2f1 Fly)
UseGatewayAvailable SinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shETV1-A
Plasmid#74973PurposeshETV1 shRNADepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HOXB13_#2
Plasmid#70094PurposeExpression of shRNA to human HOXB13, puromycin selectionDepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE
Plasmid#198054PurposeAll-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloningDepositorInsertsrTetR(SE-G72P)-VP48-T2A-mCherry-BSD
3xFLAG-insert
UseLentiviral and Synthetic BiologyTags3xFLAGExpressionMammalianPromoterTRE3G and pEFAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LYN-1/1
Plasmid#91536PurposeProtein expression and purification of human SH3 domain construct LYN-1/1DepositorInsertLYN-1/1 (LYN Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Beclin-BATS
Plasmid#30498DepositorTags3xFlag and hrGFPExpressionMammalianMutationBeclin 1 + 413-492 aa of BarkorAvailable SinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-hWIPI1A-3×FLAG
Plasmid#215509PurposeExpresses FLAG tagged human WIPI1A.DepositorInsertWD-repeat protein interacting with phosphoinositides 1A (WIPI1 Human)
UseRetroviralTags3×FLAGExpressionMammalianAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSGFPnERT(#205)
Plasmid#184065Purposecyclofen-inducible GFP nuclear relocation via mammalian cell transfection or mRNA synthesisDepositorInserteGFP-nls-ERT2
UseSynthetic BiologyExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-1574/+120
Plasmid#35539DepositorUseLuciferaseTagsluciferasePromoternoneAvailable SinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shETV1-B
Plasmid#74974PurposeshETV1 shRNADepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His K206E/K534A hTFNG
Plasmid#70133PurposeExpresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobeDepositorInsertN6His K206e/K534A hTFNG (TF Human)
TagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationchanged Lys206 to a glutamic acid and Lys534 to a…PromoterSV40Available SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
PXPR007 sgATXN1L-1
Plasmid#74962PurposeCas9 + sgATXN1L-1 with blasticidin selectionDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
PXPR007 sgATXN1L-2
Plasmid#74963PurposeCas9 + sgATXN1L-2 with blasticidin selectionDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-hSOX17
Plasmid#206389PurposeExpresses human SOX17 in mammalian cells, for lentivirus generation.DepositorInsertSOX17 (SOX17 Human)
UseLentiviralAvailable SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_CCK1.0
Plasmid#208674PurposeExpresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in a cre-dependent mannerDepositorInsertGPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
UseAAVPromoterhSynAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant
Plasmid#149507PurposeTransient expression of human MFSD12 with recoded nucleotide sequence. Dileucine motif LL256-257 has been mutated to AA.DepositorInsertMFSD12 (MFSD12 Human)
UseTransient expressionTagsTEV-3XHA-3XFLAGExpressionMammalianMutationCodon Optimized Sequence, p.LL256-257AAPromoterCMVAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES
Plasmid#182485PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NPYmut
Plasmid#208679PurposeExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in neuronsDepositorInsertGPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
UseAAVPromoterhSynAvailable SinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
MKV937/nlp-1p::FlincG3
Plasmid#140508PurposeThis construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.DepositorInsertFlincG3
ExpressionWormPromoternlp-1Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGAP32-1/1
Plasmid#91312PurposeProtein expression and purification of human SH3 domain construct ARHGAP32-1/1DepositorInsertARHGAP32-1/1 (ARHGAP32 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag RdgBbeta (long form) splice variant 1
Plasmid#58275Purposemammalian expression of human RdgBbeta splice variant 1DepositorAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP
Plasmid#108484Purposeinducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFPDepositorInsertCenpB-dCEN-INCENP (INCENP Human)
TagsEGFP and VSVExpressionMammalianMutationCEN box replaced by CenpB 1-166PromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ABL1-1/1
Plasmid#91521PurposeProtein expression and purification of human SH3 domain construct ABL1-1/1DepositorInsertABL1-1/1 (ABL1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A RIGI K270A
Plasmid#167290PurposeLentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-IDepositorInsertDDX58 (RIGI Human)
UseLentiviralTagstagBFPExpressionMammalianMutationK270APromoterSFFVAvailable SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-SV40P-Tet2 enhancer H1
Plasmid#63883PurposeLuciferase reporter plasmid used to study transcriptional control of murine Tet2DepositorInsertTet2 enhancer fragment H1
UseLuciferaseExpressionMammalianPromoterSV40Available SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoEb
Plasmid#121901PurposePlasmid for making zebrafish apoEb in situ probeDepositorInsertApoeb (apoeb Zebrafish)
UseUnspecifiedAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hALDOA D34S
Plasmid#210810PurposeTo reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)DepositorInsertALDOA (ALDOA Human)
TagsHAExpressionMammalianMutationresidues 34 of ALDOA mutated to serinePromoterCMVAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ASAP2-1/1
Plasmid#91504PurposeProtein expression and purification of human SH3 domain construct ASAP2-1/1DepositorInsertASAP2-1/1 (ASAP2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_MAP3K9-1/2
Plasmid#91313PurposeProtein expression and purification of human SH3 domain construct MAP3K9-1/2DepositorInsertMAP3K9-1/2 (MAP3K9 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only