We narrowed to 5,041 results for: U6...

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pMKO.1-shBub1

Plasmid

#160948

Purpose

Bub1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Bub1 shRNA (Bub1 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdk1

Plasmid

#160953

Purpose

Cdk1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdk1 shRNA (Cdk1 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

Plasmid

#121819

Purpose

pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP320-pAAV-U6SaCas9gRNA(CREB3)_EFS-GFP-KASH-pA

Plasmid

#113697

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pX330_sgACTA2

Plasmid

#63712

Purpose

Express sgACTA2 in mammalian cells.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

ACTA2 (ACTA2 Mouse, Human)

Expression

Mammalian

Promoter

U6

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAAVscCBPITuDlet-7Gpluc

Plasmid

#35652

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

TuD let-7

Use

AAV

Promoter

U6

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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FnoCas12a-DR-GC-swap-cr in pBCSK+

Plasmid

#126644

Purpose

Cloning vector FnoCas12a-DR-GC-swap-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnoCas12a Full Length Direct Repeat GC Swap crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 Promoter

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Stat3)-PGKpuroBFP-W

Plasmid

#105027

Purpose

Lentiviral gRNA plasmid targeting mouse Stat3 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Stat3 (Stat3 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgC1orf27-1

Plasmid

#86130

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting C1orf27

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA 1 targeting C1orf27 (C1orf27 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgGTTG.CMV/CB-Gluc

Plasmid

#121512

Purpose

Expresses sgRNA targeting the intron in the MADM alleles (GT and TG).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgGTTG

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgC1orf27-2

Plasmid

#86131

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting C1orf27

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA 2 targeting C1orf27 (C1orf27 )

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgDnmt1_1

Plasmid

#138347

Purpose

Express guide RNA 1 for Dnmt1

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

mouse Dnmt1 sgRNA (Dnmt1 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgDnmt1_2

Plasmid

#138348

Purpose

Express guide RNA 2 for mouse Dnmt1

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

mouse Dnmt1 sgRNA (Dnmt1 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pTE4561

Plasmid

#107525

Purpose

Expresses Lb crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Lb crRNA
mCherry

Expression

Mammalian

Promoter

CBh and human U6

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pORANGE C9orf4-GFP KI

Plasmid

#131472

Purpose

Endogenous tagging of C9orf4: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Frrs1l Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_055

Plasmid

#123920

Purpose

Encodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

gRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA

Plasmid

#68346

Purpose

Used to produce AAV expressing the FlpO recombinase and pig gRNA towards 3 tumor suppressors: PTEN, p53 and SMAD4

Depositor

Jannik Elverløv-Jakobsen

Article

Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

Inserts

3xgRNA;PTENA, p53B,SMAD4A
FlPO

Use

AAV; Flp/frt

Expression

Mammalian

Promoter

CAG and U6

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_hSt1Cas9_CNRZ1066

Plasmid

#136651

Purpose

A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA.

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9:CNRZ_1066 Chimera
sgRNA for St1Cas9

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG and hU6

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTBL649 CHYRON2 integration construct

Plasmid

#126446

Purpose

To integrate the CHYRON2 locus at AAVS1 in human cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6-CHYRON2 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVscCBPI TuDmiR122Gpluc7xlet7BT

Plasmid

#35648

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

TuD miR-122

Use

AAV

Promoter

U6

Available Since

May 7, 2012

Availability

Academic Institutions and Nonprofits only

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