164,060 results
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Plasmid#221900PurposeExpresses Halo7 targeted to the plasma membraneDepositorInsertLck-Halo7
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
RoL10X_pET29b
Plasmid#215427PurposeExpression construct for RoL10X gene, codon optimized for expression in E. coliDepositorInsertRoL10X
UseTagsExpressionBacterialMutationV209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F…PromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
ThcCut1_pET29b
Plasmid#215429PurposeExpression construct for ThcCut1 gene, codon optimized for expression in E. coliDepositorInsertThcCut1
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR182-DuET
Plasmid#213272PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR182 (GPR182 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-ER-PM-P2A
Plasmid#214893PurposeExpresses equimolar Plasma Membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PM Contact sitesDepositorInsertSPLICSFAST-ER-PM-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
px552-U6-gRNA1-U6-gRNA2-CMV-eGFP
Plasmid#211760PurposePaired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.DepositorInsertgRNA1 and gRNA2 targeting VEGF-A (VEGFA Mouse, Human, M. mulatta (rhesus macaque))
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0751
Plasmid#143729PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHIVEP1 (HIVEP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-AM
Plasmid#206513PurposeAAV packaging vector containing AAV2 rep and AM cap and partial AAV2 p5 promoterDepositorInsertAM capsid
UseAAVTagsExpressionMutationPromoterp5Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-Ruby2-NS
Plasmid#166569PurposeMultisite Gateway middle entry clone encoding mRuby2. Parton lab clone DJADepositorInsertRuby2
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
acta1-mRuby2-VHH[GFP]
Plasmid#163856PurposeZebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRXDepositorInsertVHH[GFP]
UseIn vitro transcription of rnaTagsRuby2ExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-Cavin4b-TurboID
Plasmid#163851PurposeIn vitro transcription of Cavin4b-TurboID. Parton lab clone JTADepositorInsertCavin4b (cavin4b Zebrafish)
UseIn vitro transcription of rnaTagsTurboIDExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-CD44b-TurboID
Plasmid#163850PurposeIn vitro transcription of CD44b-TurboID. Parton lab clone JTJDepositorInsertCD44b (cd44b Zebrafish)
UseIn vitro transcription of rnaTagsTurboIDExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-BASU-EGFP
Plasmid#163845PurposeIn vitro transcription of BASU-EGFP. Parton lab clone JRRDepositorInsertBASU
UseIn vitro transcription of rnaTagsEGFPExpressionMutationPromoterAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-TDP-43 M337V
Plasmid#162609PurposeAllows for transcription of mutant TDP-43-M337V mRNA for injection into zebrafish embryos for BiFC assaysDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionMammalianMutationM337VPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pJOE4847.2
Plasmid#135086Purposerhamnose-inducible expression vectorDepositorInsertMalE-6xHis-Ulp1
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC30A7
Plasmid#132148PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC30A7 (SLC30A7 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLENTI_RPS10_K139R
Plasmid#127137DepositorInsertRPS10 (RPS10 Human)
UseLentiviralTagsExpressionMutationK139RPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS10_K138R
Plasmid#127136DepositorInsertRPS10 (RPS10 Human)
UseLentiviralTagsExpressionMutationK138RPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOS008: GCaMP6F calcium sensor (cytosolic)
Plasmid#163045PurposeGCaMP6F calcium sensor (cytosolic)DepositorInsertGCaMP6F
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE-FLAG-NAT10-siR-WT
Plasmid#59365PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInserthuman NAT10 (NAT10 Human)
UseTagsFLAGExpressionMammalianMutationMutations to render cDNA resistant to a siRNAPromoterCMV-tetAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-rev-ChR2-tdtomato
Plasmid#18917PurposeCre-dependent viral expression of ChR2-tdTomato for neuronal excitationDepositorInsertChannelrhodopsin 2-tdtomato
UseAAV and Cre/Lox; Adeno-associated virusTagstdtomatoExpressionMutationPromoterCAGAvailable sinceFeb. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
HDAC5 Flag
Plasmid#13822PurposeMammalian expression of human histone deacetylase 5 with flag tagDepositorInsertHDAC5 (HDAC5 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NBRE3
Plasmid#208254PurposepGL3 promoter luciferase reporter vector containing 3 copies of the NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) used for luciferase reporter assays in mammalian cellsDepositorInsert3X NBRE-LUC
UseTagsExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINE GFP nanobody:Halo:His6
Plasmid#111090PurposeBacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.DepositorInsertGFP Nanobody
UseTagsHalo and His6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7 promoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mPCSK2
Plasmid#122673PurposeExpresses mouse PCSK2 in mammalian cellsDepositorInsertMus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2) (Pcsk2 Mouse)
UseTagsExpressionMammalianMutationPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CMVs.Pl.Cre.rBG (AAV8)
Viral Prep#105537-AAV8PurposeReady-to-use AAV8 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCMVTagsNoneAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Soma-jGCaMP8m (AAV1)
Viral Prep#169257-AAV1PurposeReady-to-use AAV1 particles produced from AAV-hSyn-Soma-jGCaMP8m (#169257). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8m plasmid DNA. Syn-driven expression of soma-targeted calcium sensor jGCaMP8m. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsNoneAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe3_ChR2_mCherry (AAV PHP.eB)
Viral Prep#213941-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiPVe3_ChR2_mCherry (#213941). In addition to the viral particles, you will also receive purified pAAV_BiPVe3_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the PV+ basket cell-targeting enhancer E3. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsmCherryAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only