Skip to main content
Addgene
Showing: 31 - 60 of 181 results

  2. psicheck-2-LPPRC-mod

    Plasmid
    #48168
    Purpose
    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element
    Depositor
    Vamsi Mootha
    Article
    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106
    Insert
    LRPPRC leucine rich pentatricopeptide repeat containing (LRPPRC Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    A/T mutation in the ATG of the uORF
    Promoter
    Available since
    Oct. 16, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pHR_PGK_SNIPR_ALPPL2 Hinge Notch SNIPR

    Plasmid
    #188377
    Purpose
    Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188377-plasmid-map-sequence-id-371137

    Enlarge

  14. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC

    Plasmid
    #22931
    Purpose
    Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)
    Depositor
    Alexandra Newton
    Article
    Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.
    Insert
    PHLPP1-deltaC (PHLPP1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    PHLPP-deltaC, deletion of PDZ binding domain--the…
    Promoter
    CMV
    Available since
    Feb. 4, 2010
    Availability
    Academic Institutions and Nonprofits only

  15. pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)

    Plasmid
    #188385
    Purpose
    Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188385-plasmid-map-sequence-id-371050

    Enlarge

  18. pAJE3-E7

    Plasmid
    #214359
    Purpose
    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins
    Depositor
    Ryan Mehl
    Article
    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-
    Inserts
    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    AmpR and lpp
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214359-plasmid-map-sequence-id-424910

    Enlarge

  23. pBRα

    Plasmid
    #53731
    Purpose
    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    αNTD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53731-plasmid-map-sequence-id-83909

    Enlarge

  27. pKB224

    Plasmid
    #170014
    Purpose
    Encodes LacI-controlled expression of surface-displayed NC and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    NC
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170014-plasmid-map-sequence-id-335947

    Enlarge

  29. pALS3-Ma-sfGFP-WT

    Plasmid
    #212119
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212119-plasmid-map-sequence-id-444185

    Enlarge

  30. pKB223

    Plasmid
    #170013
    Purpose
    Encodes LacI-controlled expression of surface-displayed C18G and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    C18G
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    170013-plasmid-map-sequence-id-335942

    Enlarge

Showing: 31 - 60 of 181 results