Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 4,976 results for: puc

Showing: 31 - 60 of 4976 results

  4. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68710-plasmid-map-sequence-id-123992

    Enlarge

  6. pUC21

    Plasmid
    #49787
    Purpose
    E. coli cloning vector (AmpR, high copy, blue/white selection, M13 IR)
    Depositor
    Joachim Messing
    Article
    Vieira et al Gene. 1991 Apr;100:189-94.
    Type
    Empty backbone
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    49787-plasmid-map-sequence-id-139618

    Enlarge

  7. pUC19_GZG

    Plasmid
    #117226
    Purpose
    shble flanked by an endogenous GAPDH promoter and terminator system
    Depositor
    Jackie Collier
    Insert
    shble
    Use
    Thraustochytrid expression
    Tags
    Expression
    Mutation
    Promoter
    GAPDH
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    117226-plasmid-map-sequence-id-224760

    Enlarge

  8. pucKan_ARS_pgkZeo_ncx1_mCherry_2TA_Puro_ARS

    Plasmid
    #163755
    Purpose
    piggybac transposon encoding human Ncx1 gene promoter-driven mCherry and puromycin-acetyltransferase for labelling and selection of pluripotent stem cell-derived cardiomyocytes
    Depositor
    Kurt Pfannkuche
    Article
    Hamad et al Methods Mol Biol. 2021 Oct 19. doi: 10.1
    Insert
    mCherry
    Use
    Piccybac transposon
    Tags
    puromycin-N-acetyltransferase
    Expression
    Mammalian
    Mutation
    Promoter
    human NCX1
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    163755-plasmid-map-sequence-id-326668

    Enlarge

  12. pUCX

    Plasmid
    #60681
    Purpose
    E. coli expression plasmid, useful in conjunction with pUCXKT for positive selection cloning and expression
    Depositor
    David Ackerley
    Article
    Prosser et al Biotechnol Lett. 2014 Sep 26.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60681-plasmid-map-sequence-id-108962

    Enlarge

  13. pUCXKT

    Plasmid
    #60682
    Purpose
    positive selection cloning and expression vector
    Depositor
    David Ackerley
    Article
    Prosser et al Biotechnol Lett. 2014 Sep 26.
    Type
    Empty backbone
    Use
    Positive selection e. coli expression vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60682-plasmid-map-sequence-id-108964

    Enlarge

  20. pUC19_CDC45_dTAG

    Plasmid
    #232733
    Purpose
    Generates CDC45 dTAG C ternimal knock-in cell lines as donor
    Depositor
    Junjie Chen
    Insert
    CDC45 (CDC45 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Synonymous mutation
    Promoter
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    232733-plasmid-map-sequence-id-465446

    Enlarge

  21. pUC19_GINS4_dTAG

    Plasmid
    #232735
    Purpose
    Generates GINS4 dTAG C ternimal knock-in cell lines as donor
    Depositor
    Junjie Chen
    Insert
    GINS4 (GINS4 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Synonymous mutation
    Promoter
    Available since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232735-plasmid-map-sequence-id-465448

    Enlarge

  22. pUC19_MCM2_dTAG

    Plasmid
    #232731
    Purpose
    Generates MCM2 dTAG C ternimal knock-in cell lines as donor
    Depositor
    Junjie Chen
    Insert
    MCM2 (MCM2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Synonymous mutation
    Promoter
    Available since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232731-plasmid-map-sequence-id-465447

    Enlarge

Showing: 31 - 60 of 4976 results