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Showing: 41 - 80 of 617 results

  15. pHCL149

    Plasmid
    #134457
    Purpose
    CmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.
    Depositor
    Thomas Bernhardt
    Article
    Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111
    Insert
    Para-popZ-rbs-H3H4-msfGFPN-CTM.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Para
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    134457-plasmid-map-sequence-id-270548

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  17. pHCL151

    Plasmid
    #134455
    Purpose
    TetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.
    Depositor
    Thomas Bernhardt
    Article
    Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111
    Insert
    linker-mScarlet-I for C-terminal fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    plac
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    134455-plasmid-map-sequence-id-270547

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  18. pHCL147

    Plasmid
    #134454
    Purpose
    TetR, lacI-Plac-ssdsbA-mscarC, Integrative plasmid for the expression of fully-secreted prey. C-terminal mScarlet-I fusion.
    Depositor
    Thomas Bernhardt
    Article
    Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111
    Insert
    linker-mScarlet-I for C-terminal fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    plac
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    134454-plasmid-map-sequence-id-270545

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  21. pVCS1

    Plasmid
    #231615
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS1 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al bioRxiv 2024.07.20.604438
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pvan/Pcym
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231615-plasmid-map-sequence-id-464094

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  22. pVCS9

    Plasmid
    #231623
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS3 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al bioRxiv 2024.07.20.604438
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pvan/Pcym
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231623-plasmid-map-sequence-id-464087

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  23. pVCS8

    Plasmid
    #231622
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS2 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al bioRxiv 2024.07.20.604438
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pvan/Pcym
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231622-plasmid-map-sequence-id-464071

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  24. pVCS6

    Plasmid
    #231620
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS3 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al bioRxiv 2024.07.20.604438
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pvan/Pcym
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231620-plasmid-map-sequence-id-464093

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  25. pVCS5

    Plasmid
    #231619
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS2 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al bioRxiv 2024.07.20.604438
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pvan/Pcym
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231619-plasmid-map-sequence-id-464091

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  26. pVCS4

    Plasmid
    #231618
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS1 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al bioRxiv 2024.07.20.604438
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pvan/Pcym
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231618-plasmid-map-sequence-id-464053

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  27. pVCS3

    Plasmid
    #231617
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS3 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al bioRxiv 2024.07.20.604438
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pvan/Pcym
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231617-plasmid-map-sequence-id-464096

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  28. pVCS2

    Plasmid
    #231616
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS2 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al bioRxiv 2024.07.20.604438
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pvan/Pcym
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231616-plasmid-map-sequence-id-464095

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  29. pS5

    Plasmid
    #231614
    Purpose
    L-arabinose (Ara)- and anhydrotetracycline (aTc)-inducible inverter circuit reported by mKate and/or sfGFP fluorescence signals
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al mSystems. 2024 Sep 17;9(9):e0084924. doi
    Insert
    Pbad-TetR-sfGFP-Ptet-AraC-mKate2
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pbad/Ptet
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231614-plasmid-map-sequence-id-464090

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  30. pS4

    Plasmid
    #231613
    Purpose
    L-arabinose (Ara)- and anhydrotetracycline (aTc)-inducible inverter circuit reported by mKate and/or sfGFP fluorescence signals
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al Biodes Res. 2023 Aug 16;5:0016. doi: 10.
    Insert
    Ptet-AraC-mKate2-Pbad-TetR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pbad/Ptet
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231613-plasmid-map-sequence-id-464070

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  34. pRecT_2

    Plasmid
    #167546
    Purpose
    Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Insert
    E. faecium Com15 recT under an IPTG inducible promoter, with lacI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    IPTG
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167546-plasmid-map-sequence-id-332204

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  35. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167547-plasmid-map-sequence-id-332733

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Showing: 41 - 80 of 617 results