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E. coli BL21 (DE3) ΔEntD

Bacterial Strain

#192874

Purpose

Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

Depositor

Christophe Guilhot, Christian Chalut

Article

Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

Bacterial Resistance

None

Species

Escherichia coli

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only
CC1215

Bacterial Strain

#79062

Purpose

BL21(DE3)-based E. coli purine auxotrophic host strain used for the preparation of PurE proteins in a purE background.

Depositor

T. J. Kappock

Bacterial Resistance

Kanamycin

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only
Anti-VIP [L108/92R] (Recombinant Antibody)

Antibody

#207161-rAb

Purpose

Anti-VIP (Human) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended Applications

Immunocytochemistry

Reactivity

Human and Rat

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available since

April 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits
Human sgRNA library Brunello in lentiGuide-Puro

Pooled Library

#73178

Purpose

Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Has Service

Lentiviral Prep

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
Human sgRNA library Brunello in lentiCRISPRv2

Pooled Library

#73179

Purpose

Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Has Service

Lentiviral Prep

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
Mouse sgRNA library Brie in lentiCRISPRv2

Pooled Library

#73632

Purpose

Mouse sgRNA library in backbone lentiCRISPRv2 containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
Mouse sgRNA library Brie in lentiGuide-Puro

Pooled Library

#73633

Purpose

Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Has Service

Lentiviral Prep

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
Mouse metabolism knockout library

Pooled Library

#160129

Purpose

CRISPR library targets mouse metabolic genes

Depositor

Kivanc Birsoy

Article

Zhu et al Cell Metab. 2020 Oct 28. pii: S1550-4131

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only
Human Lipid Droplet and Metabolism Library

Pooled Library

#191535

Purpose

Lentiviral CRISPR library targeting 1,196 genes related to lipid droplet biology and cell metabolism.

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only
Syn61

Bacterial Strain

#174513

Purpose

Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

Depositor

Jason W Chin

Article

Fredens et al Nature. 2019 May;569(7757):514-518. doi:

Bacterial Resistance

Streptomycin

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only
Anti-Ankyrin-G [N106/36] (Recombinant Antibody)

Antibody

#192039-rAb

Purpose

Anti-Ankyrin-G (Human) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended Applications

Immunocytochemistry and Immunohistochemistry

Reactivity

Human, Mouse, and Rat

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available since

Feb. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits
Human CRISPR Poison Exon Knockout Library

Pooled Library

#138084

Purpose

The human pgPoison library induces skipping of “poison” cassette exons and corresponding upstream constitutive exons by targeting 3' splice sites with paired gRNAs (pgRNAs).

Depositor

Robert Bradley

Article

Thomas et al Nat Genet. 2020 Jan;52(1):84-94. doi: 10

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
Anti-Arl13b [N295B/66R] (Recombinant Antibody)

Antibody

#180085-rAb

Purpose

Anti-Arl13b (Mouse) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended Applications

Immunohistochemistry and Western Blot

Reactivity

Mouse and Rat

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available since

March 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits
TP53 MITE Library

Pooled Library

#113569

Purpose

Pooled lentiviral plasmid library containing ~8,000 single amino acid substitution and nonsense mutant variants of p53

Depositor

David Root, William Hahn

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Expression

Mammalian

Species

Homo sapiens

Use

Lentiviral

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only
Syn61Δ3(ev5)

Bacterial Strain

#174514

Purpose

Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Bacterial Resistance

Streptomycin

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only
Mouse genome-wide lentiviral CRISPR gRNA library version 2

Pooled Library

#67988

Purpose

Mouse genome-wide CRISPR guide RNA library V2. (pKLV2-U6gRNA5(MouseV2)-PGKpuro2ABFP-W)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Species

Homo sapiens, Mus musculus, and Synthetic

Use

CRISPR and Lentiviral

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only
Human genome-wide lentiviral CRISPR gRNA library version 1

Pooled Library

#67989

Purpose

Human genome-wide CRISPR guide RNA library V1 (pKLV2-U6gRNA5(HumanV1)-PGKpuro2ABFP-W)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Species

Homo sapiens, Mus musculus, and Synthetic

Use

CRISPR and Lentiviral

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only
Green monkey (Chlorocebus sabaeus) sgRNA library

Pooled Library

#178284

Purpose

Genome-wide CRISPR screening library targeting 20,991 genes in C. sabaeus, 84,963 total gRNAs

Depositor

David Root, John Doench

Article

Wei et al Cell. 2021 Jan 7;184(1):76-91.e13. doi:

Expression

Mammalian

Species

Chlorocebus sabaeus (african green monkey)

Use

CRISPR and Lentiviral

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only
Mouse Toronto KnockOut (mTKO) CRISPR Library - lentiCRISPRv2

Pooled Library

#159392

Purpose

Mouse lentiviral genome-wide CRISPR library

Depositor

Jason Moffat

Article

Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only
Mouse Toronto KnockOut (mTKO) CRISPR Library - pLCKO2

Pooled Library

#159393

Purpose

Mouse lentiviral genome-wide CRISPR library

Depositor

Jason Moffat

Article

Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only
Mouse Validation (mVAL) CRISPR Library

Pooled Library

#159391

Purpose

Pooled CRISPR library targets core intrinsic immune evasion genes

Depositor

Jason Moffat

Article

Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only
Mouse sphingolipid metabolism knockout library

Pooled Library

#206249

Purpose

The sphingolipid metabolism library targets 82 mouse genes in the de novo ceramide and downstream sphingolipid metabolism pathways.

Depositor

Kivanc Birsoy

Article

Soula et al Nature. 2024 Aug 7. doi: 10.1038/s41586-

Use

CRISPR and Lentiviral

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only
Mode #2 Library (14-3-3σ)

Pooled Library

#111706

Purpose

Encodes 14-3-3σ and human phosphopeptides fused to split mCherry for Hi-P

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
Mode #2 Library (14-3-3β)

Pooled Library

#111705

Purpose

Encodes 14-3-3β and human phosphopeptides fused to split mCherry for Hi-P

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
Mode #1 Library

Pooled Library

#111704

Purpose

Encodes GST-fused human phosphopeptides

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
E. coli MEV20

Bacterial Strain

#197113

Purpose

E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Bacterial Resistance

None

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only
E. coli MEV15

Bacterial Strain

#197112

Purpose

E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Bacterial Resistance

None

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only
Mode #2 Library (NEDD4-2 WW2 domain)

Pooled Library

#111708

Purpose

Encodes NEDD4-2 WW2 domain and human phosphopeptides fused to split mCherry for Hi-P

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
Mode #2 Library (NEDD4 WW2 domain)

Pooled Library

#111707

Purpose

Encodes NEDD4 WW2 domain and human phosphopeptides fused to split mCherry for Hi-P

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655 RARE

Bacterial Strain

#61440

Purpose

This E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes

Depositor

Kristala Prather

Article

Kunjapur et al J Am Chem Soc. 2014 Aug 20;136(33):11644

Bacterial Resistance

None

Available since

Feb. 1, 2015

Availability

Academic Institutions and Nonprofits only
Barcode Library 0 (BCL0)

Pooled Library

#179851

Purpose

Lentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code ACGT.

Depositor

Jason Moffat

Article

Rehman et al Cell. 2021 Jan 7;184(1):226-242.e21. doi

Expression

Mammalian

Use

Lentiviral

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only
Barcode Library 1 (BCL1)

Pooled Library

#179852

Purpose

Lentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code CCAA.

Depositor

Jason Moffat

Article

Rehman et al Cell. 2021 Jan 7;184(1):226-242.e21. doi

Expression

Mammalian

Use

Lentiviral

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only
pMuSIC pool v1.0

Pooled Library

#227193

Purpose

Two-way barcodes from 18 fluorescent proteins for mammalian expression. Can be used for clone tracing, or for pairing with perturbations for single cell resolution in spectral detection measurements.

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Expression

Mammalian

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only
Human Whole Genome sgRNA iBAR Library

Pooled Library

#140633

Purpose

CRISPR pooled screen at high MOI by the embedded internal barcodes

Depositor

Wensheng Wei

Article

Zhu et al Genome Biol. 2019 Jan 24;20(1):20. doi:

Use

CRISPR and Lentiviral

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only
pSMAL-CellTag-multi-v1 barcode library

Pooled Library

#206045

Purpose

The CellTag multi library of barcodes can be used to clonally label cells with DNA barcodes. These barcodes can be profiled directly with single-cell RNA and ATAC sequencing, to allow clonal tracking

Depositor

Samantha Morris

Article

Jindal et al Nat Biotechnol. 2023 Sep 25. doi: 10.103

Use

Lentiviral

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 Spike (S) Ectodomain Library C

Pooled Library

#157973

Purpose

Encodes Spike ectodomain mutations that stabilize 'up' prefusion conformation

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Expression

Bacterial and Yeast

Species

Sars-cov-2

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 Spike (S) Ectodomain Library B

Pooled Library

#157972

Purpose

Encodes Spike ectodomain mutations that stabilize 'up' prefusion conformation

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Expression

Bacterial and Yeast

Species

Sars-cov-2

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 Spike (S) Ectodomain Library A

Pooled Library

#157971

Purpose

Encodes Spike ectodomain mutations that stabilize 'up' prefusion conformation

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Expression

Bacterial and Yeast

Species

Sars-cov-2

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only
Lenti-human-UBDUB-gRNA-puromycin-IRES-CFP

Pooled Library

#171531

Purpose

Pooled gRNA library against human ubiquitination and de-ubiquitination complex genes (UBDUB)

Depositor

Chunliang Li

Article

Wright et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Expression

Mammalian

Species

Homo sapiens

Use

Lentiviral

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only
HB101 endA::frt

Bacterial Strain

#45473

Depositor

Michele Swanson

Article

Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

Bacterial Resistance

Streptomycin

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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