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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 112 results for: dCas9-VPR

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Showing: 21 - 40 of 112 results

  7. M_ST1n_VPR

    Plasmid
    #63799
    Purpose
    ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector
    Depositor
    George Church
    Publication
    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ST1-dCas9-VPR
    Use
    CRISPR and Synthetic Biology
    Tags
    VPR (VP64-p65-Rta)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    63799-plasmid-map-sequence-id-106659

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  10. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid
    #166693
    Purpose
    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.
    Depositor
    Elvir Becirovic
    Publication
    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    TRE and U6
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    166693-plasmid-map-sequence-id-331043

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  11. pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

    Plasmid
    #84239
    Purpose
    Expresses ABA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABI-tagBFP-Sp dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84239-plasmid-map-sequence-id-160811

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  12. pSH231-EF1-BLST-dCas9-VPR

    Plasmid
    #115148
    Purpose
    Safe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassette
    Depositor
    Raymond Monnat, Michael Phelps
    Publication
    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BlastR-P2A-dCas9-VPR
    Use
    CRISPR
    Tags
    Nucleoplasmin NLS and SV40NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    115148-plasmid-map-sequence-id-233281

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  13. pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

    Plasmid
    #140199
    Purpose
    Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Promoter
    PgpdA
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140199-plasmid-map-sequence-id-309723

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  14. pdCas9-GFP-VPR

    Plasmid
    #181907
    Purpose
    Fluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-Rta
    Depositor
    Karsten Rippe
    Publication
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VPR
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181907-plasmid-map-sequence-id-359690

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  15. pPB-CAG-dCas9-VPR-wpA-pPuroTK

    Plasmid
    #214127
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VPR
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214127-plasmid-map-sequence-id-424240

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  18. JEH127

    Plasmid
    #179299
    Purpose
    Expresses dCas9 fused to VPR
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VPR
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically inactive Cas9)
    Promoter
    CAG
    Available since
    Feb. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    179299-plasmid-map-sequence-id-354392

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Showing: 21 - 40 of 112 results