We narrowed to 4,386 results for: pcas9

1. pCas9-VRER_2A_GFP

   Plasmid

   #75475

   Purpose

   Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

   Depositor

   Marc Tessier-Lavigne

   Article

   Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

   Insert

   Cas9-VRER
   

   Use

   CRISPR

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   CAG

   Available since

   May 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCas9-J23109

   Plasmid

   #113150

   Purpose

   Plasmid constitutively expressing Cas9 protein

   Depositor

   Chong Zhang

   Article

   Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCas9-J23113

   Plasmid

   #119701

   Purpose

   Expression of Cas9 with a weak promoter

   Depositor

   Chong Zhang

   Article

   Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCas9-CR4

   Plasmid

   #62655

   Purpose

   Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   Cas9 nuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCas9-CtIP

   Plasmid

   #109403

   Purpose

   Expresses Cas9 fusion to human CtIP protein

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   CtIP (RBBP8 Human)
   

   Use

   Tags

   Expression

   Mutation

   full length CtIP

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCas9-Geminin

   Plasmid

   #109401

   Purpose

   Expresses Cas9 fusion to Geminin degron domain

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   Geminin (GMNN Human)
   

   Use

   Tags

   Expression

   Mutation

   Geminin degron fragment

   Promoter

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCas9-HE

   Plasmid

   #109400

   Purpose

   Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   HDR-Enhancer domain of human CtIP protein (RBBP8 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCas9(D10A)

   Plasmid

   #74495

   Purpose

   Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

   Insert

   Cas9 D10A
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   D10A

   Promoter

   pTeto

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCas9-polyA

   Plasmid

   #72602

   Purpose

   express hCas9 byT7 promoter with polyadenine tails

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

   Insert

   Cas9-polyadenine
   

   Use

   Tags

   81-bp polyadenylation

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAS9-TPC

    Plasmid

    #61478

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PcUbi4-2

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCas9-beta

    Plasmid

    #169240

    Purpose

    Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Inserts

    cas9
    beta
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCas9-CAT

    Plasmid

    #80323

    Purpose

    Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Inserts

    Cas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR

    Tags

    3X FLAG and NLS

    Expression

    Mutation

    Promoter

    TgTUB1

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCas9-VRER_2A_GFP

    Plasmid

    #75475

    Purpose

    Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

    Depositor

    Marc Tessier-Lavigne

    Article

    Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

    Insert

    Cas9-VRER
    

    Use

    CRISPR

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    CAG

    Available since

    May 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCas9-J23109

    Plasmid

    #113150

    Purpose

    Plasmid constitutively expressing Cas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ADEPT-pCas9

    Plasmid

    #238032

    Purpose

    Encodes Cas9 (ssrA degradation-tagged) under a tetracycline promoter (pTet) on a low-copy replication origin (SC101) as part of the ADEPT system.

    Depositor

    Lingchong You

    Article

    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

    Insert

    spCas9
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Mutation

    Promoter

    tet

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCas9-J23113

    Plasmid

    #119701

    Purpose

    Expression of Cas9 with a weak promoter

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Plasmid

    #101217

    Purpose

    Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCas9_GFP

    Plasmid

    #44719

    Purpose

    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Cas9-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAS9i

    Plasmid

    #141249

    Purpose

    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SpCas9

    Plasmid

    #188489

    Purpose

    Expresses FLAG tagged SpCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge