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pBS mNotch1-IC (DM#270)

Plasmid

#15079

Depositor

Douglas Melton

Article

Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

Insert

Notch1-IC (Notch1 Mouse)

Expression

Bacterial

Mutation

K1750A

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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FG12

Plasmid

#14884

Depositor

David Baltimore

Article

Qin et al Proc Natl Acad Sci U S A. 2003 Jan 7. 10

Insert

flap-Ub promoter-GFP-WRE

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2007

Availability

Academic Institutions and Nonprofits only

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pKJ0464

Plasmid

#228716

Purpose

Bacterial expression of T7 RNAP with E643G mutation; for protein production and purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

SUmo_T7pol_E643G

Expression

Bacterial

Mutation

E643G

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Hook1-EGFP

Plasmid

#171941

Purpose

Expresses Hook1 in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Hook1

Tags

EGFP

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAM5087

Plasmid

#85123

Purpose

Ptrc-YFP subcloned from pAM5080 into NSII-Km vector.

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

YFP

Expression

Bacterial

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV.IRES.hCD4

Plasmid

#35712

Depositor

Patricia Ernst

Article

Artinger et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

Inserts

IRES
hCD4

Use

Retroviral

Expression

Mammalian

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
act5c-LbCas12a

Plasmid

#140620

Purpose

Ubiquitous expression of LbCas12a in Drosophila

Depositor

Michael Boutros

Article

Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

Insert

LbCas12a

Use

CRISPR

Expression

Insect

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pP2BA

Plasmid

#31369

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Per2 (Per2 Mouse)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
Stat4 pRc/CMV

Plasmid

#8711

Depositor

Jim Darnell

Article

Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

Insert

Stat4 (Stat4 Mouse)

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pAWp28

Plasmid

#73850

Purpose

pBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Type

Empty backbone

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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mRFP1-pBAD

Plasmid

#54667

Purpose

Localization: Protein Expression Vector , Excitation: 584, Emission: 607

Depositor

Robert Campbell, Michael Davidson, Roger Tsien

Article

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Type

Empty backbone

Tags

6xHis and mRFP1

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pETconNK

Plasmid

#81169

Purpose

pETconNK Empty Backbone

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Type

Empty backbone

Tags

c-myc

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBS Zpapc

Plasmid

#25774

Depositor

Edward De Robertis

Article

Yamamoto et al Development. 1998 Sep . 125(17):3389-97.

Insert

Papc (pcdh8 Zebrafish)

Expression

Bacterial

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
pGEX-2T SHP1 WT

Plasmid

#8594

Depositor

Ben Neel

Article

Plutzky et al Proc Natl Acad Sci U S A 1992 Feb 1;89(3

Insert

SHP1 (PTPN6 Human)

Tags

GST

Expression

Bacterial

Available Since

June 21, 2005

Availability

Academic Institutions and Nonprofits only
MSCV FLIP FF

Plasmid

#19744

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting Firefly luciferase

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pTA-attB

Plasmid

#18937

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 attB

Expression

Bacterial

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

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pKEW397_AcrIIA15

Plasmid

#138285

Purpose

Expression of AcrIIA15 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA15

Use

CRISPR

Tags

MBP

Expression

Bacterial

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pKEW394_AcrIIA14

Plasmid

#138283

Purpose

Expression of AcrIIA14 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA14

Use

CRISPR

Tags

SFGFP

Expression

Bacterial

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTH-Cen15-mVenus

Plasmid

#49642

Purpose

TALEColor-mVenus-Chromosome 15-specific Centromere

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Insert

TALEColor-Cen15

Use

TALEN

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pTH-Cen18-mVenus

Plasmid

#49641

Purpose

TALEColor-mVenus-Chromosome 18-specific Centromere

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Insert

TALEColor-Cen18

Use

TALEN

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pTA-attP

Plasmid

#18939

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 attP

Expression

Bacterial

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

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pKJ0376

Plasmid

#228711

Purpose

Mammalian expression of human codon-optimized Bxb1 mutant N194G

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

Bxb1_N194G

Expression

Mammalian

Mutation

N194G

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-Cygnet2.1

Plasmid

#19737

Depositor

Wolfgang Dostmann

Article

Honda et al Proc Natl Acad Sci U S A. 2001 Feb 27. 9

Insert

Cygnet 2.1

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-Nck1

Plasmid

#45892

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Nck-1 (NCK1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
p3521 pET ubcH6

Plasmid

#8653

Depositor

Peter Howley

Article

Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

Insert

UbcH6 (UBE2E1 Human)

Expression

Bacterial

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pLPC 12S E1A

Plasmid

#18740

Depositor

Scott Lowe

Article

Samuelson et al Proc Natl Acad Sci U S A. 1997 Oct 28. 9

Insert

12S E1A

Use

Retroviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-miR26a2

Plasmid

#21115

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

miR-26a2 (MIR26A2 Human)

Expression

Mammalian

Available Since

May 18, 2009

Availability

Academic Institutions and Nonprofits only
pEntr-Nkx2-5flbio

Plasmid

#32969

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

Nkx2-5 (Nkx2-5 Mouse)

Use

Gateway

Tags

flag-bio

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
humanMER20MER39

Plasmid

#35256

Depositor

Gunter Wagner

Article

Emera et al Proc Natl Acad Sci U S A. 2012 Jul 10;10

Insert

MER20/MER39

Use

Luciferase

Promoter

dPRL promoter

Available Since

March 19, 2012

Availability

Academic Institutions and Nonprofits only
pBSSK mPTP-1B

Plasmid

#8603

Depositor

Ben Neel

Article

Chernoff et al Proc Natl Acad Sci U S A 1990 Apr;87(7):

Insert

PTP-1B (Ptpn1 Mouse)

Expression

Bacterial

Available Since

June 21, 2005

Availability

Academic Institutions and Nonprofits only
CamKII alpha Venus

Plasmid

#29427

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CamKII alpha

Tags

Venus

Expression

Mammalian

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
prGT3

Plasmid

#15993

Depositor

Morris Birnbaum

Article

Birnbaum et al Proc Natl Acad Sci U S A. 1986 Aug . 83(

Insert

GLUT1 (Slc2a1 Rat)

Expression

Bacterial

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA h-MLSN

Plasmid

#21083

Depositor

Craig Montell

Article

Xu et al Proc Natl Acad Sci U S A. 2001 Sep 11. 9

Insert

TrpM1 (TRPM1 Human)

Tags

Myc

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pJFRC170-3XUAS-IVS-Cre::PEST

Plasmid

#32144

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

Cre::PEST

Expression

Insect

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pKEW215_AcrIIA13

Plasmid

#138281

Purpose

Expression of AcrIIA13 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA13

Use

CRISPR

Tags

MBP

Expression

Bacterial

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF525-AcrIIA5_gb36_lenti

Plasmid

#138306

Purpose

Lenti expression of AcrIIA5

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA5

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CUP Ent2 YFP p316

Plasmid

#15593

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

ENT2 (ENT2 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pNFPBA

Plasmid

#31370

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Per2 (Per2 Mouse)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pPSSUB-101

Plasmid

#26580

Depositor

Ron Weiss

Article

Basu et al Proc Natl Acad Sci U S A. 2004 Apr 27. 1

Insert

destabilized GFP

Expression

Bacterial

Mutation

S2R, C-terminal extension RPAANDENYA(LVA)

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK11-01

Plasmid

#99605

Purpose

Plasmid containing parBp-mKate2 fusion

Depositor

Morten Kjos, Jan-Willem Veening

Article

van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

Insert

parBp-mKate2

Use

Synthetic Biology

Tags

mKate2

Promoter

PczcD

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
SNX9

Plasmid

#171943

Purpose

Lentiviral plasmid to express mouse SNX9 in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

SNX9

Use

Lentiviral

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK17-01

Plasmid

#99603

Purpose

Plasmid containing the parB-m(sf)gfp fusion

Depositor

Morten Kjos, Jan-Willem Veening

Article

van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

Insert

parBp

Use

Synthetic Biology

Tags

m(sf)gfp

Promoter

PczcD

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pVS45

Plasmid

#20059

Depositor

Stephen Sligar

Article

Beck von Bodman et al Proc Natl Acad Sci U S A. 1986 Dec . 83(

Insert

rat hepatic cytochrome b5

Expression

Bacterial

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3 N-MLSN

Plasmid

#21082

Depositor

Craig Montell

Article

Xu et al Proc Natl Acad Sci U S A. 2001 Sep 11. 9

Insert

TRPM1 (TRPM1 Human)

Tags

Myc

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
234_pSKB3-Venus-LUMP

Plasmid

#65975

Purpose

Bacterial expression of Venus-LUMP FRET pair

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Tags

Venus

Expression

Bacterial

Promoter

T7

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CUP Rnq1 YFP p316

Plasmid

#15596

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

RNQ1 (RNQ1 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pFastBac-Giardia Dicer

Plasmid

#89146

Purpose

Expression of Giardia Dicer in Sf9 cells

Depositor

Jennifer Doudna

Article

MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

Insert

Giardia Dicer

Tags

His6-teve

Expression

Insect

Available Since

April 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDW114

Plasmid

#70061

Purpose

artificial transcription template "wild-type"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' - repeat cassette - E. coli rpoB - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
glgP-gap1-

Plasmid

#101838

Purpose

deletes glgP_gap1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kanamycin casette (in place of synpcc7942_244_245 operon)

Use

Cloning vector

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDW115

Plasmid

#70062

Purpose

artificial transcription template "pause ablation"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' A+2G T+6G - repeat cassette - E. coli rpoB - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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