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pLenti CMV Neo DEST (705-1)

Plasmid

#17392

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

46 Articles

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3xAP1pGL3 (3xAP-1 in pGL3-basic)

Plasmid

#40342

Depositor

Alexander Dent

Publication

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

3xAP-1

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains three canonical AP-1 binding sites (TGAC…

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

57 Articles

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pRK5-EGFP-Tau P301L

Plasmid

#46908

Purpose

expresses EGFP tagged Tau P301L in mammalian cells

Depositor

Karen Ashe

Publication

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau P301L (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

P301L

Promoter

CMV

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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pXR004: CasRx pre-gRNA cloning backbone

Plasmid

#109054

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

Depositor

Patrick Hsu

Publication

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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T7opt in pCAGGS

Plasmid

#65974

Purpose

Human codon-optimized T7 polymerase

Depositor

Benhur Lee

Publication

Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

Insert

T7-opt

Expression

Mammalian

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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lentiCRISPRv2 neo

Plasmid

#98292

Purpose

Variant of lentiCRISPRv2 that confers G418 resistance

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pINDUCER21 (ORF-EG)

Plasmid

#46948

Purpose

Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

Depositor

Stephen Elledge, Thomas Westbrook

Publication

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Type

Empty backbone

Use

Lentiviral

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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pLV hUbC-Cas9-T2A-GFP

Plasmid

#53190

Purpose

3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

Depositor

Charles Gersbach

Publication

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

hUbC

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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OKSIM

Plasmid

#24603

Depositor

Jose Cibelli

Publication

Ross et al Stem Cells Dev. 2010 Aug;19(8):1221-9

Insert

Oct4 KLF4 Sox2 c-Myc (SOX2, POU5F1, MYC, KLF4 Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

Oct4-2A-KLF4-2A-Sox2-IRES-Myc

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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ABE8e

Plasmid

#138489

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9

Expression

Mammalian

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

54 Articles

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pLOX-TERT-iresTK

Plasmid

#12245

Purpose

2nd generation lentiviral vector for reversible immortalization of human primary cells

Depositor

Didier Trono

Publication

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

TERT, HSV1-TK (TERT Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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Ef1a_Large T-antigen_Ires_Puro

Plasmid

#18922

Depositor

Linzhao Cheng

Publication

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

Large T Antigen (SV40gp6 Simian virus 40)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pSL-MS2-12X

Plasmid

#27119

Depositor

Robert Singer

Publication

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

MS2 binding sites (12)

Expression

Bacterial

Available since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pSin-EF2-LIN28-Pur

Plasmid

#16580

Depositor

James Thomson

Publication

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens lin-28 homolog (C. elegans) (LIN28A Human)

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pSLCAR-CD19-BBz

Plasmid

#135992

Purpose

Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Publication

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a-short

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pLEX_305-N-dTAG

Plasmid

#91797

Purpose

Lentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system

Depositor

James Bradner, Behnam Nabet

Publication

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

hPGK

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pC015 - dLwCas13a-NF

Plasmid

#91905

Purpose

Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

dLwCas13a

Use

CRISPR

Tags

NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

Expression

Mammalian

Mutation

R474A and R1046A

Promoter

CAG

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEP4 E02S CK2M EN2L

Plasmid

#20924

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28

Depositor

James Thomson

Publication

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (MYC, SOX2, KLF4, NANOG, POU5F1, LIN28A Human)

Use

Episomal/ebna

Available since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pCSCMV:tdTomato

Plasmid

#30530

Purpose

mammalian expression of tdTomato controlled by the ubiquitously active CMV promoter.

Depositor

Gerhart Ryffel

Publication

Waldner et al BMC Dev Biol. 2009 Jun 23;9:37. doi: 10.

Insert

tdTomato

Expression

Mammalian

Mutation

R415H

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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shp53 pLKO.1 puro

Plasmid

#19119

Purpose

3rd gen lentiviral vector for knocking down p53 gene expression

Depositor

Bob Weinberg

Publication

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

small hairpin RNA against tumor protein p53 (TP53 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Sept. 9, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

57 Articles

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