We narrowed to 59,342 results for: cloning

1. pME nls BFP FLAG WPRE bGH pA (JDW 484)

   Plasmid

   #224539

   Purpose

   A Gateway compatible middle entry clone containing a 3x-NLS-mTagBFP2-FLAG for labeling the nucleus

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   nls-mTagBFP2-FLAG_WPRE-bGH-pA
   

   Use

   Gateway cloning

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-EF1a-pA (JDW 1222)

   Plasmid

   #224526

   Purpose

   A Gateway compatible 3' entry clone containing rat EF-1a polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EF-1a polyA
   

   Use

   Gateway cloning

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3E-WPRE-bGH-pA (JDW 1221)

   Plasmid

   #224527

   Purpose

   A Gateway compatible 3' entry clone containing a WPRE upstream of Bovine growth hormone polyA to stabilize mRN

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   WPRE-bGH
   

   Use

   Gateway cloning

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5E-dr_Unc (JDW 1365)

   Plasmid

   #224482

   Purpose

   Gateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intron

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Unc-503 promoter
   

   Use

   Gateway cloning

   Available Since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-H2B-V5-n2StayGoldC4 (JDW 1354)

   Plasmid

   #224486

   Purpose

   Gateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   H2B-V5-n2StayGoldC4
   

   Use

   Gateway cloning

   Available Since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME V5 mScarlet-I (JDW 1323)

   Plasmid

   #224487

   Purpose

   Gateway compatible middle entry clone containing V5 tagged m-Scarlet-I (Cytosolic far red fluorescent reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   V5-mScarlet-I
   

   Use

   Gateway cloning

   Available Since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-myr-mTagBFP2-Flag (JDW 1183)

   Plasmid

   #224492

   Purpose

   Gateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   myr-mTagBFP2-Flag
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-Lifeact-mCherry-3xHA (JDW 1321)

   Plasmid

   #224493

   Purpose

   Gateway compatible middle entry clone containing Lifeact-mCherry (RFP F-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Lifeact-mCherry-3xHA
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-mCerulean3-TUBB1A (JDW 1367)

   Plasmid

   #224503

   Purpose

   Gateway compatible middle entry clone containing mCerulean3 fused to TUBB1A for visualizing tubulin in cells

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mCerulean3-TUBB1A
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME TAEL2.0-5xC120-minpro (JDW 1109)

    Plasmid

    #224507

    Purpose

    Gateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    TAEL2.0-5xC120
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-TAEL (JDW 1324)

    Plasmid

    #224508

    Purpose

    Gateway compatible middle entry clone containing TAEL2.0 transcription factor for making binary TAEL system vectors (generating a tissue specific driver)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    TAEL
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-Myc-BioID2-MCS1 (JDW 1095)

    Plasmid

    #224510

    Purpose

    Gateway compatible middle entry clone containing a Myc-tagged BIOID2 construct

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Myc-BioID2
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-Myc-BioID2-MCS2 (JDW 1209)

    Plasmid

    #224511

    Purpose

    A Gateway compatible middle clone with a Myc-tagged BIOID2 construct followed by a flexible linker and an MCS

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Myc-BioID2
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3E-V5-mClover3-pA (JDW 1318)

    Plasmid

    #224530

    Purpose

    A Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mClover3-pA
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)

    Plasmid

    #224532

    Purpose

    A Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    IRES-H2A-mCherry-SV40pA
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3E EFS-rtTA/rtTA3 (JDW 1214)

    Plasmid

    #224533

    Purpose

    A Gateway compatible 3' entry clone containing the Human EFS promoter driving rtTA-3rd generation transactivator

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    rtTA
    

    Use

    Gateway cloning

    Promoter

    EFS

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3E-Actin-Vhh-mNeonGreen-HA (JDW 1225)

    Plasmid

    #224534

    Purpose

    A Gateway compatible 3' entry clone containing an Actin nanobody fused to mNeonGreen with a c-terminal HA tag

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-mNeonGreen-HA
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3E-IRES-mTagBFP2-pA (JDW 1361)

    Plasmid

    #224535

    Purpose

    A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    IRES-FLAG-NLS-mTagBFP2-pA
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3E-IRES-(n2)StayGold(c4)-pA (JDW 1364)

    Plasmid

    #224537

    Purpose

    A Gateway compatible 3' entry clone containing IRES-(n2)StayGold(c4)-pA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    IRES-(n2)StayGold(c4)-pA
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221-Lifeact- mScarlet-HA (JDW 1247)

    Plasmid

    #224538

    Purpose

    A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Lifeact-mScarlet-HA
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge