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p3E-EF1a-pA (JDW 1222)

Plasmid

#224526

Purpose

A Gateway compatible 3' entry clone containing rat EF-1a polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EF-1a polyA

Use

Gateway cloning

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pME AmCyan (JDW 1151)

Plasmid

#224485

Purpose

Gateway compatible middle entry clone containing AmCyan cytosolic blue flourescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

AmCyan

Use

Gateway cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mCherry-3xHA (JDW 1321)

Plasmid

#224493

Purpose

Gateway compatible middle entry clone containing Lifeact-mCherry (RFP F-actin reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lifeact-mCherry-3xHA

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-mCerulean3-TUBB1A (JDW 1367)

Plasmid

#224503

Purpose

Gateway compatible middle entry clone containing mCerulean3 fused to TUBB1A for visualizing tubulin in cells

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean3-TUBB1A

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-myc-BirA (JDW 1210)

Plasmid

#224509

Purpose

Gateway compatible middle entry clone containing a Myc tagged BirA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Myc-BirA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Myc-BioID2-MCS1 (JDW 1095)

Plasmid

#224510

Purpose

Gateway compatible middle entry clone containing a Myc-tagged BIOID2 construct

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Myc-BioID2

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Myc-BioID2-MCS2 (JDW 1209)

Plasmid

#224511

Purpose

A Gateway compatible middle clone with a Myc-tagged BIOID2 construct followed by a flexible linker and an MCS

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Myc-BioID2

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-V5-mClover3-pA (JDW 1318)

Plasmid

#224530

Purpose

A Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mClover3-pA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT021

Plasmid

#225172

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (GmR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBlueKan+cysMPro

Plasmid

#187879

Purpose

Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.

Depositor

Gaylen Uhlich

Article

Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

Type

Empty backbone

Expression

Bacterial

Promoter

CysM

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pHBJT017

Plasmid

#225168

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (AmpR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT024

Plasmid

#225175

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (SmR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT023

Plasmid

#225174

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (SmR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT019

Plasmid

#225170

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (TetR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT020

Plasmid

#225171

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (TetR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT022

Plasmid

#225173

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (GmR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT016

Plasmid

#225167

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (ChlR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT015

Plasmid

#225166

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (ChlR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT018

Plasmid

#225169

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (AmpR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pPtPBR11_fcpShble_GWB

Plasmid

#154031

Purpose

Gateway cloning into P. tricornutum episomal vector

Depositor

Raffaela Abbriano

Type

Empty backbone

Use

Diatom cloning

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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