We narrowed to 5,312 results for: pcDNA3.1(+)

1. pCDNA3-SREBP-2

   Plasmid

   #204200

   Purpose

   Mammalian expression of SREBP-2

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-2 (SREBF2 Human)
   

   Use

   Tags

   T7x3

   Expression

   Mammalian

   Mutation

   silent mutations

   Promoter

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAL113

   Plasmid

   #22471

   Purpose

   Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PhyB NT (PHYB Mustard Weed, Mouse)
   

   Use

   Synthetic Biology

   Tags

   10aaLinker-mCFP-HrasCT

   Expression

   Mammalian

   Mutation

   Y276H

   Promoter

   CMV

   Available Since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. CanlonicSF

   Plasmid

   #178342

   Purpose

   To explore lactate dynamics in intact systems

   Depositor

   Alejandro San Martín

   Article

   Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

   Insert

   CanlonicSF
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-Climp63(1–336)

   Plasmid

   #186957

   Purpose

   Expresses mCherry-Climp63 with partial luminal domain

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   Ckap4 (Ckap4 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Only remain partial luminal domain

   Promoter

   CMV Promoter

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-FlicR1

   Plasmid

   #74142

   Purpose

   Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

   Depositor

   Robert Campbell

   Article

   Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

   Insert

   FlicR1
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. RA-NES

   Plasmid

   #61019

   Purpose

   ddRFP A-NES

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A
   

   Use

   Tags

   HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-LiMETER-v2

   Plasmid

   #113934

   Purpose

   Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

   Depositor

   Yubin Zhou

   Article

   He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

   Insert

   LOV2 and Stim1 polybasic domain (PB)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cytosolic FAK biosensor

   Plasmid

   #78300

   Purpose

   FRET biosensor. Visualization of cytosolic FAK activity in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

   Insert

   Cytosolic FAK biosensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 Lats1 (Nigg HS189)

   Plasmid

   #41156

   Depositor

   Erich Nigg

   Article

   Chan et al Oncogene. 2005 Mar 17;24(12):2076-86.

   Insert

   Lats1 (LATS1 Human)
   

   Use

   Tags

   3xMyc and PreScission Site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

10. mKO3-TEAD4

    Plasmid

    #221217

    Purpose

    RFP fused TEAD4

    Depositor

    Xiaokun Shu

    Article

    Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

    Insert

    mKO3-TEAD4 (TEAD4 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA SF-HERC2 F4

    Plasmid

    #55787

    Purpose

    Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)
    

    Use

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 24, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA SF-HERC2 F5

    Plasmid

    #55788

    Purpose

    Expresses N-terminal 3xFLAG/STREP tagged HERC2 (3550-4450aa)

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (3550-4450aa) (HERC2 Human)
    

    Use

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 24, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA_RGG-GFP-RGG

    Plasmid

    #124930

    Purpose

    Expression of RGG-GFP-RGG

    Depositor

    Matthew Good, Benjamin Schuster, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-BaLgp120-8his

    Plasmid

    #100925

    Purpose

    Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BaL) Env
    

    Use

    Tags

    CD5 leader sequence and Purification tag after K5…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Luc-noPS

    Plasmid

    #177940

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 HA Arf1 ActQ71L

    Plasmid

    #10832

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf1 Q71L (ARF1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q71L. Defective in GTP hydrolysis. Constitutive…

    Promoter

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

    Plasmid

    #137827

    Purpose

    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTE4396

    Plasmid

    #74041

    Purpose

    Expresses human codon-optimized AsCpf1 and As crRNA.

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    As crRNA
    AsCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. HiBiT-FZD1

    Plasmid

    #195839

    Purpose

    Expresses human FZD1 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD1 (FZD1 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-noPS

    Plasmid

    #177943

    Purpose

    Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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