We narrowed to 15,226 results for: NOL

1. pSV(X) C/EBPbeta

   Plasmid

   #13393

   Depositor

   Richard Schwartz

   Article

   Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

   Insert

   C/EBPbeta (Cebpb Rat)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

2. LLP689_L2_1IF2_FRT-OCS_Rluc_C1_FlpF2

   Plasmid

   #192412

   Purpose

   To act as a 1-input state of the split-AND gate.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLS

   Plasmid

   #192413

   Purpose

   To act as a 1-input state of the split-AND gate.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP

   Plasmid

   #194934

   Purpose

   LV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptor

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   antiFITC-LaG16-sNRR(Y186F/Y229A)-SynNotch-Gal4-VP64-T2A-BFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3A9cbetaTCR.HSV2.3

   Plasmid

   #69594

   Purpose

   TCR beta chain genomic transgenic construct for generation of gBT-I mice

   Depositor

   Francis R. Carbone

   Article

   Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

   Insert

   TCR beta VJ region from clone HSV2.3 (Tcrb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pU6nH1-HD10kb

   Plasmid

   #172657

   Purpose

   Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene

   Depositor

   Jay Shendure

   Article

   Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   pegRNA-HD10kbA/pegRNA-HD10kbB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCellFree_G08

   Plasmid

   #67142

   Purpose

   Gateway destination vector for cell-free expression with C-term mCherry myc-tag tag

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

   Type

   Empty backbone

   Use

   Cell-free expression

   Tags

   His-MYC and mCherry

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. TAL2274

   Plasmid

   #36711

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   MDM2-TALEN_Left (Mdm2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pAC1748

   Plasmid

   #127982

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available since

   Dec. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pH-AFID-3

    Plasmid

    #167361

    Purpose

    For inducing predictable deletions in rice plants

    Depositor

    Caixia Gao

    Article

    Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

    Insert

    A3A-Cas9-UDG-T2A-AP lyase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TAL2384

    Plasmid

    #36793

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BRCA1-TALEN_Left (Brca1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pSV(X) C/EBPalpha

    Plasmid

    #13392

    Depositor

    Richard Schwartz

    Article

    Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

    Insert

    C/EBPalpha (Cebpa Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pMK407 (BSD-P2A-mAID-Nluc)

    Plasmid

    #121197

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    BSD-P2A-mAID-Nluc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EK0354 SFFV-mCherry-SG(Bdnf.s1L72)SG-EGFP (FLP-IN)

    Plasmid

    #191151

    Purpose

    Expression of a 72 bp long sensor for murine Bdnf 3' UTR in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s1L72:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUCBB-ptet-eGFP

    Plasmid

    #32554

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    ptet

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 AT1R DRY-AAY

    Plasmid

    #45759

    Depositor

    Laszlo Hunyady, Robert Lefkowitz

    Article

    Gaborik et al Endocrinology. 2003 Jun;144(6):2220-8.

    Insert

    AT1R DRY-AAY (Agtr1a Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed amino acids 125-126 from DR to AA

    Promoter

    CMV

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pCfB6684

    Plasmid

    #106154

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCfB6631

    Plasmid

    #106161

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pASKt15C+SrtA7woH

    Plasmid

    #65020

    Purpose

    Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, E105K, E108A, D16…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOTF3-AK (clone3)

    Plasmid

    #21165

    Depositor

    James Thomson

    Article

    Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

    Insert

    POUF5F1 (genomic DNA) (POU5F1 Human)
    

    Use

    Cre/Lox; Homologous recombination

    Tags

    Expression

    Mutation

    NeoR, EGFP, and SV40 polyA are between the two ge…

    Promoter

    Available since

    June 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUCBB-pBAD-eGFP

    Plasmid

    #32553

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSCALPS_miR-146aSponge_mCherry_GFP

    Plasmid

    #149718

    Purpose

    Lentiviral vector expressing a miR-146a decoy sponge

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    14X miR-146a binding sites
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G to T mutation in position 75 of the insert

    Promoter

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGL3-DSCP-Gateway

    Plasmid

    #71507

    Purpose

    flySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the DSCP core promoter

    Depositor

    Alexander Stark

    Article

    Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

    Type

    Empty backbone

    Use

    Flystarr-seq validation vector

    Tags

    luciferase

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MSP2266

    Plasmid

    #70705

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCellFree_G03 PDGFRB

    Plasmid

    #67130

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pMJA179

    Plasmid

    #128537

    Purpose

    Expresses anti-Prunus dulcis scFv Ab

    Depositor

    Marcos Alcocer

    Article

    de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

    Insert

    scFv Ab clone PD1F6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. KG#59

    Plasmid

    #110929

    Purpose

    Expresses proteins pan-neuronally in C. elegans (entire nervous system)

    Depositor

    Kenneth Miller

    Article

    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

    Inserts

    rab-3 promoter
    unc-54 3' control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Dppa1-T2A-H2B-Halo donor

    Plasmid

    #113118

    Purpose

    Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Dppa1 donor region including homology arms and T2A-H2B-Halo
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. IBMc233

    Plasmid

    #161939

    Purpose

    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PPhlF

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pColE1_sgRNA_M7NS_AmpR

    Plasmid

    #119157

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge