We narrowed to 34,877 results for: cas

1. pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP

   Plasmid

   #164131

   Purpose

   Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   SpCas9 (cas9 Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-NLS-ddMisCas13b-SNAPf-NLS-3xFlag

   Plasmid

   #191537

   Purpose

   Overexpression of NLS-ddMisCas13b-SNAPf-NLS-3xFlag in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-ddMisCas13b-SNAPf-NLS-3xFlag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls

   Plasmid

   #64113

   Purpose

   Expression of St1 dCas9-3XGFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV hUbC-dSaCas9-KRAB-T2A-PuroR

   Plasmid

   #106249

   Purpose

   Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

   Insert

   dead S. aureus Cas9 KRAB T2A PuroR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA-NLS and NLS-KRAB

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   hUbC

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

   Plasmid

   #162334

   Purpose

   Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   dSaCas9-KRAB
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

   Plasmid

   #220493

   Purpose

   To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

   Depositor

   Sébastien Britton

   Article

   Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

   Insert

   sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

   Plasmid

   #115694

   Purpose

   All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.

   Depositor

   Erik Sontheimer

   Article

   Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

   Inserts

   NmeCas9
   Nme-sgRNA
   

   Use

   CRISPR

   Tags

   NLS and NLS, HA

   Expression

   Insect and Mammalian

   Mutation

   None and fusion of repeat/tracrRNA to make a sgRNA

   Promoter

   EF-1 alpha and U6

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pST1374-N-NLS-flag-linker-Cas9-H840A

   Plasmid

   #51129

   Purpose

   Cas9 nickase optimized for nuclear import.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Insert

   Cas9 nickase
   

   Use

   CRISPR

   Tags

   Flag and NLS

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   CMV

   Available since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)

   Plasmid

   #139990

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   VQR=D1135V/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pST1374-N-NLS-flag-linker-Cas9-D10A

    Plasmid

    #51130

    Purpose

    Cas9 nickase optimized for nuclear import.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Insert

    Cas9 nickase
    

    Use

    CRISPR

    Tags

    Flag and NLS

    Expression

    Mammalian

    Mutation

    D10A (nickase mutant)

    Promoter

    CMV

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDF0387 pcmv-hudiscas7-11-r978-gggs-r1294

    Plasmid

    #186984

    Purpose

    mammalian expression of cas7-11-r978-gggs-r1294

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    cas7-11-r978-gggs-r1294
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    r978-gggs-r1294 deletion

    Promoter

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)

    Plasmid

    #139993

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)

    Plasmid

    #139991

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    VRER=D1135V/G1218R/R1335E/T1337R

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

    Plasmid

    #139992

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    VRQR=D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX408 Francisella tularensis subsp. novicida Cas9

    Plasmid

    #68705

    Purpose

    Francisella tularensis subsp. novicida Cas9

    Depositor

    Feng Zhang

    Insert

    FtCas9
    

    Use

    CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4

    Plasmid

    #107310

    Purpose

    Encoding dCas9-10xGCN4 driven by dSV40 promoter

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Insert

    dSV40-dCas9-10xGCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA

    Plasmid

    #86986

    Purpose

    For cloning and expression of sgRNA together with expression of a Cas9-Venus fusion protein

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Insert

    Cas9-venus
    

    Use

    Tags

    Cas9-Venus fusion protein

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

18. p23-NES-LwaCas13a-msfGFP-NES-Flag

    Plasmid

    #165070

    Purpose

    overexpression of LwaCas13a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    LwaCas13a
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-NLS-dMisCas13b-SNAPf-NLS-3xFlag

    Plasmid

    #191536

    Purpose

    Overexpression of NLS-dMisCas13b-SNAPf-NLS-3xFlag in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-dMisCas13b-SNAPf-NLS-3xFlag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLD037-pCMV-APOBEC-Cas9(D10A)-rXRCC1

    Plasmid

    #165444

    Purpose

    Expresses ACX, rXRCC1 in mammalian cells

    Depositor

    Wei Leong Chew

    Article

    Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

    Insert

    APOBEC-nCas9-rXRCC1 (Apobec1 Streptococcus pyogenes, Synthetic, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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