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pCS2+ centrin-RFP

Plasmid

#26753

Depositor

Christopher Kintner

Article

Park et al Nat Genet. 2008 Jul . 40(7):871-9.

Insert

Centrin 4 (cetn4 Frog)

Use

Reporter for basal body

Tags

monomeric RFP

Available Since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pET23-CBD-(-PP)-EGFP-H246,249D

Plasmid

#12598

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Type

Empty backbone

Tags

6xHis and EGFP

Expression

Bacterial

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
HA-FOXO3A S253A

Plasmid

#8349

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Tags

HA

Expression

Mammalian

Mutation

S253A

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only
pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

Plasmid

#214885

Purpose

Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-OC-IRES-BSD

Plasmid

#53118

Purpose

To create stable cell clones with high-level expression of Cas9 and OCT1

Depositor

Wensheng Wei

Article

Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Inserts

OCT1 (POU2F1 Human)
Cas9

Use

Lentiviral

Tags

IRES-BSD, NLS, and P2A

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only
pCMMP-2xmyc-Bcl2A1-IRES-mRFP

Plasmid

#36973

Depositor

Bill Sugden

Article

Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

Insert

BCL2A1 (BCL2A1 Human)

Use

Retroviral

Tags

2xMYC

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only
pWZL-neo delta-p85

Plasmid

#10888

Depositor

William Hahn

Article

Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Insert

delta-p85 (PIK3R1 Bovine)

Use

Retroviral

Expression

Mammalian

Mutation

Deletion of aa 478-513

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
His-GFP SNAP25

Plasmid

#170872

Purpose

Mammalian expression of His-GFP SNAP25.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

SNAP 25 (Snap25 Rat)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pS2513·PHP

Plasmid

#122590

Purpose

Constitutive expressed pHiorine2 for pH quan. in gram-negative cells

Depositor

Pablo Ivan Nikel

Article

Arce-Rodriguez et al Microb Biotechnol. 2019 Jun 4. doi: 10.1

Insert

phIorine2

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pem7

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCW-Flag-CtIP-T847E

Plasmid

#71110

Purpose

mammalian expession of Flag-CtlP T847E mutation

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

T847E

Promoter

TRE promoter, Tet ON

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn Con/Foff EYFP

Plasmid

#55651

Purpose

Cre-on/Flp-off EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV; Cre on/flp off eyfp

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRS-QUE7mu

Plasmid

#129311

Purpose

Low concentration ATP measurement

Depositor

Hiroyuki Noji

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-7mu

Expression

Bacterial

Available Since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTrig

Plasmid

#104576

Purpose

Copy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under Lac promoter for copy increase.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PLac-repL

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSuper mouse Dicer3

Plasmid

#14778

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (Dicer1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pATetO 6XHis T5 Exonuclease

Plasmid

#206209

Purpose

Inducible expression of T5 exonuclease

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

Insert

T5 exonuclease

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pS448∙CsR

Plasmid

#122096

Purpose

CRISPR/Cas9 counterselection in Gram-negative bacteria

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Inserts

Cas9
sgRNA

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pem7 and Pm

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mCebpa

Plasmid

#79551

Purpose

Expression of mouse Cebpa

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Cebp alpha (Cebpa Mouse)

Use

Lentiviral

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pHsp83-NLS-HA-2xMCP-2xTagRFP-T

Plasmid

#71242

Purpose

Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

NLS-2xMCP-2xTagRFP-T

Use

P element-mediated germline transformation

Tags

TagRFP-T

Expression

Insect

Promoter

Hsp83

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pSR651

Plasmid

#65213

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Article

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-1 cap

Use

AAV

Expression

Insect

Promoter

p10 and polyhedrin

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pMCL-HA-MAPKK1-8E (K97M)

Plasmid

#40811

Depositor

Natalie Ahn

Article

Mansour et al Science. 1994 Aug 12;265(5174):966-70.

Insert

MAP2K1 (MAP2K1 Human)

Tags

HA

Expression

Mammalian

Mutation

K97M

Promoter

CMV

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only