We narrowed to 13,280 results for: cDNA

1. pcDNA3 HA-PHLPP1 full length

   Plasmid

   #37100

   Purpose

   Expresses HA tagged, full-length human PHLPP1 in mammalian cells

   Depositor

   Alexandra Newton

   Article

   Warfel et al J Biol Chem. 2011 Jun 3;286(22):19777-88

   Insert

   PHLPP1 (PHLPP1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-Delta(Pro-MP)ADAM17-HA

   Plasmid

   #65221

   Purpose

   Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

   Insert

   Delta(Pro-MP)ADAM17 (ADAM17 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted AA 18-473

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1(+)-HLA-Flag-natT1R3

   Plasmid

   #113950

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R3 (TAS1R3 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from HLA class I …

   Expression

   Mammalian

   Mutation

   residues 21-852

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-hAsCpf1(TYCV) (pY210)

   Plasmid

   #89351

   Purpose

   Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K607R

   Promoter

   CMV

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-N-Flag-NLRP3 91-710

   Plasmid

   #75140

   Purpose

   Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   fragment encoding amino acids 91-710

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-ATR Kinase dead

   Plasmid

   #31612

   Depositor

   Aziz Sancar

   Article

   Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

   Insert

   ATR (ATR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K2327R (kinase dead)

   Promoter

   Available since

   Nov. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-EGFP-Rac1(Q61L)

   Plasmid

   #13720

   Depositor

   Klaus Hahn

   Article

   Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

   Insert

   EGFP-Rac1(Q61L) (RAC1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-Lyso-ExRai-AMPKAR

   Plasmid

   #192449

   Purpose

   ExRai-AMPKAR targeted to the lysosomal membrane.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   Lyso-ExRai-AMPKAR
   

   Use

   Tags

   Full-length LAMP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-Flag-H3.3-polyA

   Plasmid

   #59780

   Purpose

   Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   H3F3B (H3-3B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA flag PPAR gamma

    Plasmid

    #8895

    Purpose

    Mammalian expression of flag-tagged PPAR gamma

    Depositor

    Bruce Spiegelman

    Article

    Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

    Insert

    PPAR gamma (Pparg Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA3-HA-HA-human CMYC S62A

    Plasmid

    #203436

    Purpose

    Mammalian expression of MYC

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    2 x HA

    Expression

    Mammalian

    Mutation

    S62A

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-neo-cterminal-3HA

    Plasmid

    #102643

    Purpose

    To generate three copies of the HA epitope tag on cDNAs.

    Depositor

    Michael E. Wright

    Type

    Empty backbone

    Use

    Tags

    3x HA (YPYDVPDYA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1/hChR2(H134R)-mCherry

    Plasmid

    #20938

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3puro-EGFP-FXR1-isoform b

    Plasmid

    #225449

    Purpose

    Express EGFP-fusion protein of FXR1 isoform b

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform b (FXR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.4-GFP-TEV-B55

    Plasmid

    #217378

    Purpose

    expresses GFP tagged B55 in mammalian cells; tev cleavable

    Depositor

    Wolfgang Peti

    Article

    Padi et al Nature. 2024 Jan;625(7993):195-203. doi:

    Insert

    B55 (PPP2R2A Human)
    

    Use

    Tags

    EGFP TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-FRT/T0-Flag-RECQL5

    Plasmid

    #222620

    Purpose

    Mammalian expression vector of flag-tagged RECQL5

    Depositor

    Daniel Durocher

    Article

    Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

    Insert

    RECQL5 (RECQL5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pcDNA3.0-Magneto2.0-p2A-mCherry

    Plasmid

    #74308

    Purpose

    Bicistronic expression of Magneto2.0 and mCherry in mammalian cells

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Insert

    Magneto2.0 (FTL, FTH1, Trpv4 Human, Rat)
    

    Use

    Tags

    Flag and p2A-mCherry

    Expression

    Mammalian

    Mutation

    TRPV4: delta 760–871

    Promoter

    CMV

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1/Myc-His(-)-HSulf-2

    Plasmid

    #13004

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 2 (SULF2 Human)
    

    Use

    Tags

    6x His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

    Plasmid

    #194683

    Purpose

    CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_05-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1481 pcDNA3 flag HA 14-3-3 zeta

    Plasmid

    #9002

    Depositor

    William Sellers

    Insert

    14-3-3 zeta (YWHAZ Bovine)
    

    Use

    Tags

    HA and flag

    Expression

    Mammalian

    Mutation

    T226A

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge