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pet28a-His6-Halo-TEV-Nrf2

Plasmid

#62455

Purpose

Halo-His6 tagged Nrf2 for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Nrf2 (NFE2L2 Human)

Tags

His6-Halo-TEV

Expression

Bacterial

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3

Plasmid

#108912

Purpose

Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Has Service

AAV9

Insert

ChroME-ST

Use

AAV

Promoter

CAG

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

Plasmid

#137830

Purpose

MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

Tags

3x FLAG

Expression

Mammalian

Mutation

Mutated Leucine 71 to Lysine in CTEVp fragment

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMZ828

Plasmid

#155322

Purpose

Constitutive expression of PhyB(1-650)-VP16 in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 S15D

Plasmid

#69005

Purpose

mammalian expression of human p53 S15D mutant

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Expression

Mammalian

Mutation

S15D

Promoter

CMV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
PT7lacO-His6-AlaRS

Plasmid

#224362

Purpose

Express AlaRS protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

AlaRS (alaS E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Intron-Tagging-pX330-Cas9-Blast

Plasmid

#159741

Purpose

Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

Depositor

Stefan Kubicek

Article

Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

Insert

donor plasmid targeting sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 S15A

Plasmid

#69004

Purpose

mammalian expression of human p53 S15A mutant.

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Expression

Mammalian

Mutation

S15A

Promoter

CMV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pMTL3

Plasmid

#127966

Purpose

constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

AAVS1-CAG-KRAB-dCas9-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDule2-Acd82

Plasmid

#174100

Purpose

Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

Depositor

Ryan Mehl

Article

Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

Insert

Acridone 82 tRNA synthetase and tRNA

Expression

Bacterial

Mutation

N311S, C313G, V366A, W382T

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCK-Drosha(UniProt)-FLAG

Plasmid

#164690

Purpose

Express Drosha-FLAG in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

Drosha (DROSHA Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

Plasmid

#50943

Purpose

Bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Insert

mRuby2-P2A-GCaMP6f

Use

AAV

Promoter

hSyn1

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-TVAmCherry-2A-oG

Plasmid

#102985

Purpose

AAV expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG) after CRE-mediated recombination

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

TVA-mCherry fusion protein after CRE-mediated recombination
Optimised Rabies Glycoprotein (oG) after CRE-mediated recombination

Use

AAV; Adeno associated viral vector

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-Lck-FRB-mTurquoise2

Plasmid

#176130

Purpose

Plasma membrane localized FRB, rapamycin heterodimerization

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

FRB

Use

Lentiviral

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAdSh.PGK.Cas9

Plasmid

#58253

Purpose

Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

Human codon-optimized S. pyogenes Cas9

Use

Adenoviral and CRISPR

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP2A

Plasmid

#115330

Purpose

expresses mCherry-CHMP2A in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2A (CHMP2A Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S

Plasmid

#62458

Purpose

expresses his-tagged C151S human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteine 151 of Keap1 to serine

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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h-Plekhm1_EGFP

Plasmid

#73836

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Tags

GFP

Expression

Mammalian

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP2B

Plasmid

#115331

Purpose

expresses mCherry-CHMP2B in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2B (CHMP2B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

silent mutation in position 4205 C to T

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-Gx-SB

Plasmid

#220125

Purpose

Expresses the 1.3-kDa Small BiT (SB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx).

Depositor

Maarten Merkx

Article

Ni et al Nat Commun. 2021 Jul 28;12(1):4586. doi:

Insert

Gx-SB

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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