167,795 results

1. pTriEx-mCherry-LOV2 V416I

   Plasmid

   #81045

   Purpose

   mammalian expression of LOV V416I

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOV V416I
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   V416I

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYeDP60-hATP13A2-E343A

   Plasmid

   #171378

   Purpose

   A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

   Depositor

   Peter Vangheluwe

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   human ATP13A2 variant 2 wild-type
   

   Tags

   BAD tag

   Expression

   Yeast

   Mutation

   E343A

   Promoter

   URA3

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pnCas9-miniSdd6-PBE

   Plasmid

   #204858

   Purpose

   For cytosine base editing using miniSdd6 in monocotyledon plants (transient transformation)

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd6-nSpCas9(D10A)-UGI
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A in SpCas9

   Promoter

   ZmUbi-1

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pS848∙CsR

   Plasmid

   #199240

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Gm resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   PEM7

   Available Since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pvalb-2A-Cre targeting vector

   Plasmid

   #61570

   Purpose

   Target the Cre recombinase gene to the stop codon of the mouse Pvalb gene

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Pvalb exon 4 - 2A - Cre
   

   Use

   Cre/Lox and Mouse Targeting

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPRE

   Plasmid

   #140229

   Purpose

   Dox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a driven rtTA and GFP

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   AntiCRISPR protein AcrVA1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-CRY2-mCherry

   Plasmid

   #140571

   Purpose

   For use in light-induced protein inactivation through the interaction with CIB1

   Depositor

   Heidi Hehnly

   Article

   Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. His6-SpyCatcher-mi3

   Plasmid

   #113043

   Purpose

   Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

   Insert

   His6-SpyCatcher-mi3
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TMPRSS3_pCSdest

   Plasmid

   #53839

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   TMPRSS3 (TMPRSS3 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

10. 4xHRE-MinTK-CRE

    Plasmid

    #141144

    Purpose

    lentiviral expression vector to generate a hypoxia fate-mapping system

    Depositor

    Daniele Gilkes

    Article

    Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

    Insert

    4xHRE-MinTK-CRE
    

    Use

    Lentiviral

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.hSyn.iGluSnFR3.v857.GPI (AAV1)

    Viral Prep

    #178331-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.hSyn.iGluSnFR3.v857.GPI (#178331). In addition to the viral particles, you will also receive purified pAAV.hSyn.iGluSnFR3.v857.GPI plasmid DNA. Synapsin-driven, third generation glutamate sensor iGluSnFR3, variant 857. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

12. pEM705 Gst (Cys)

    Plasmid

    #109376

    Purpose

    GsX chimera with Gαt C-terminus (Pertussis toxin sensitive)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gst chimera
    

    Tags

    None

    Expression

    Mammalian

    Mutation

    Cys at position 391 (Pertussis Toxin Sensitive)

    Promoter

    CAG

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2-CIB1-mCerulean-Rab11

    Plasmid

    #140574

    Purpose

    For use in light-induced protein inactivation through the interaction with CRY2

    Depositor

    Heidi Hehnly

    Article

    Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

    Inserts

    CIB1 (CIB1 Mustard Weed)
    Rab11A (RAB11A Human)
    

    Tags

    mCerulean

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJT304 Ec07 Lenti pEF-attB LSR GFP

    Plasmid

    #184947

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Patrick Hsu, Michael Bassik, Ami Bhatt, Lacra Bintu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Ec07 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1

    Plasmid

    #193356

    Purpose

    VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 (KLF4, SOX2, POU5F1 Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Mutation

    SOX2-17 is engineered highly cooperative chimeric…

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFH2.16_MiniGagZip-MCP_Zip-P2A-eGFP

    Plasmid

    #205531

    Purpose

    Express HIV MiniGagZip-MCP_Zip-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    MiniGagZip-MCP_Zip
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pInt_attP1_chlorR

    Plasmid

    #205295

    Purpose

    Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA4.TO-ORF39-2xCSTREP

    Plasmid

    #136200

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF39

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF39
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pgWIZ-SGCAgl

    Plasmid

    #131456

    Purpose

    Encodes Alpha Sarcoglycan 3xFLAG tagged protein (Therapeutic gene) to cure LGMD2D and luciferase enzyme for in vivo live imaging

    Depositor

    Michele Calos

    Article

    Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

    Insert

    SGCA (SGCA Human)
    

    Tags

    3xFLAG

    Expression

    Bacterial and Mammalian

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. VSVG-YFP-3xUVR8

    Plasmid

    #49801

    Purpose

    expresses VSVG fused to UVR8 for light-triggered trafficking

    Depositor

    Matthew Kennedy

    Article

    Chen et al J Cell Biol. 2013 May 13;201(4):631-40.

    Insert

    VSVG-YFP-3xUVR8
    

    Tags

    HA, MYC, and YFP

    Expression

    Mammalian

    Mutation

    VSVG contains ts045 mutations

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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