We narrowed to 18,346 results for: multi
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Plasmid#171182PurposeConstitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1DepositorInsertCas12f1
UseCRISPRExpressionBacterialPromotercas9 promoter from Streptococcus pyogenesAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-TNFa-RUSH
Plasmid#166901PurposeRUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-HaloDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHaloTag
ExpressionMammalianAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PE7_AAVS1_knockin_HDR_donor
Plasmid#223001PurposeHDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPE7
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_hU6
Plasmid#102840PurposeCan be used as PCR template for a STAgR reactionDepositorInsertgRNAScaffold_hU6promoter
PromoterhU6Available sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7goldRNA
Plasmid#47930PurposeIn vitro transcription of golden gRNADepositorInsertgolden gRNA (slc24a5 Zebrafish)
UseCRISPRAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG79-attL1-pegRNA-attL2
Plasmid#213050PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Geminin-mStrawberry reporter
Plasmid#158690PurposeThis plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoSuperfolderGFP-SpHis5
Plasmid#44837PurposeYeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with SpHis5 selectionDepositorInsertyoSuperfolderGFP
ExpressionYeastAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-PEmax-P2A-Puro
Plasmid#228890PurposeLentiviral delivery and expression of PEmax protein with puromycin selection cassette.DepositorInsertsPEmax
Puromycin resistance
UseCRISPR and LentiviralExpressionMammalianAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV-F2A-T2A-Venus
Plasmid#62621PurposeAdEasy Shuttle vector modified for multicistronic expression. Contains T2A, F2A and Venus tagDepositorInsertMCS with F2A and T2A
UseAdenoviralTagsVenusExpressionMammalianPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJT119b
Plasmid#50551PurposeExpresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoterDepositorInsertsCcaS
CcaR
sfGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS-hPhyB-mCherry-HRasCT
Plasmid#100281PurposeExpresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPhytochrome B (PHYB Mustard Weed)
TagsmCherry fused with HRas C-terminus for plasma mem…ExpressionMammalianMutationHuman codon optimized PhyB (1-908 aa)PromoterCAG promoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5155
Plasmid#175391PurposeLow Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)DepositorTypeEmpty backboneUseMouse Targeting; Cloning vector, to be used to de…Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pISH-beta2-microglobulin
Plasmid#15883DepositorInsertbeta2-microglobulin (B2m Mouse)
Available sinceJan. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAJE3-E7
Plasmid#214359PurposeExpresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteinsDepositorInserts2nd generation Tet2 "E7" aminoacyl tRNA synthetase
Lpp promoted M. jannaschii tRNA
Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
Orthogonal "D4II" ColE1 origin
ExpressionBacterialPromoterAmpR and lppAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKRG3-mU6-PUb-hSpCas9-pAc
Plasmid#162163PurposeExpresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectableDepositorInsertshSpCas9
sgRNA
UseCRISPRTagsT2A-pAcExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAID 1.1 N-T2A-Bsr
Plasmid#105985PurposeTo make an auxin inducible degron mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertT2A-Bsr
ExpressionMammalianPromoterCMVAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro
Plasmid#74947PurposeDosage control and tracing of reprogramming episomesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOct3/4 (POU5F1 Human)
ExpressionMammalianAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJ3 SHP2 E76A
Plasmid#8321DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSHP2 (PTPN11 Human)
ExpressionMammalianMutationE76A (activated mutant)Available sinceJune 17, 2005AvailabilityAcademic Institutions and Nonprofits only -
pME-mTagBFP
Plasmid#75148PurposeTagBFP (blue) fluorophore for use with 5’ promoters.DepositorPublicationInsertmTagBFP
UseMiddle entry vector for multisite gateway three f…Promotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only