We narrowed to 17,911 results for: por

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pcDNA3-ExRai-AMPKAR(T/A)

Plasmid

#192447

Purpose

Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR(T/A)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Mutation

Thr 6 in AMPK substrate mutated to Ala.

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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mKO2-SLBP(18-126)

Plasmid

#83914

Purpose

Fluorescent probe for S/G2 cell cycle transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mKO2-SLBP(18-126) (SLBP Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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SPLICS PM-ER Short P2A

Plasmid

#164112

Purpose

Detect the short Plasma membrane-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP PM-ER Short P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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YY120: pPB_TRE::GEMINI(B)-p2A-GEMINI(A)

Plasmid

#228881

Purpose

PiggyBac plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a doxycycline-inducible promotor.

Depositor

Dingchang Lin

Article

Yan et al Nature. 2026 Mar 3. doi: 10.1038/s41586-

Insert

Building blocks of GEMINI for intracellular assembly

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FlipCherry-T2A-GFP

Plasmid

#124436

Purpose

Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipCherry-T2A-GFP

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-H2-Kb SCT-T2A-GFP

Plasmid

#205454

Purpose

lentiviral plasmid for expression of mouse H2-Kb SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

H2-Kb SCT (H2-K1 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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SPLICS PM-ER Long P2A

Plasmid

#164111

Purpose

Detect the long Plasma membrane-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP PM-ER Long P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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human STIM1 CFP

Plasmid

#19755

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

CFP

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV6-AC EJ7-GFP

Plasmid

#113617

Purpose

Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

EJ7-GFP variant of EGFP

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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SPLICS LY-MT Short P2A

Plasmid

#213614

Purpose

Detect the short-range Lysosome-Mitochondria contact

Depositor

Tito Calì

Article

Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

Insert

SPLICS LY-MT Short P2A

Expression

Mammalian

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo-NES

Plasmid

#199545

Purpose

expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

NES and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-VPS35-mScarlet

Plasmid

#215474

Purpose

Expression vector with VPS35 tagged with mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

VPS35 (VPS35 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pUB-GFP

Plasmid

#11155

Purpose

Mammalian expression vector for expression of GFP (Ubiquitin C promoter)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

Ubiquitin C promoter (UBC Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSmApple

Plasmid

#191259

Purpose

Bacterial expression plasmids for Long Stokes shift red fluorescent protein LSSmApple

Depositor

Robert Campbell

Article

Ejike et al Biochemistry. 2024 Jan 2;63(1):171-180.

Insert

LSSmApple

Tags

His tag

Expression

Bacterial

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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SPLICS LY-MT Long P2A

Plasmid

#213613

Purpose

Detect the long-range Lysosome-Mitochondria contact

Depositor

Tito Calì

Article

Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

Insert

SPLICS LY-MT Long P2A

Expression

Mammalian

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-Sec24D

Plasmid

#226581

Purpose

Encodes Sec24D protein labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Westrate et al J Cell Sci. 2020 Jul 24;133(14). pii: jc

Insert

Sec24D (Sec24 homolog D) (SEC24D Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMK089

Plasmid

#213964

Purpose

Dual-Reporter Plasmid for mRNA 5'UTR Massively Parallel Reporter Assays

Depositor

Hani Goodarzi

Article

Khoroshkin et al Nat Methods. 2024 Sep;21(9):1634-1645. d

Insert

5'UTR cloning site

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pSimple-miR302/367

Plasmid

#98748

Purpose

Episomal reprogramming

Depositor

Sara Howden, James Thomson

Article

Howden et al Stem Cell Reports. 2015 Dec 8;5(6):1109-

Insert

miR302/367 (miRNA cluster) (MIR302A, MIR367 Human)

Expression

Mammalian

Promoter

EF1alpha

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pSJX023

Plasmid

#232326

Purpose

Genome editing in Caulobacter crescentus

Depositor

Wei Zhao

Article

Sun et al Nucleic Acids Res. 2025 Apr 22;53(8):gka

Inserts

sgRNA
SpCas9M

Use

CRISPR

Tags

H-arms and sfGFP

Expression

Bacterial

Promoter

PJ23119 and Pxyl

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST

Plasmid

#105448

Purpose

Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector

Depositor

Hillel Adesnik

Article

Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

Has Service

AAV9

Insert

ChrimsonR-mRuby2-ST

Use

AAV

Tags

mRuby2 and soma targeting motif

Mutation

K176R in Chrimson

Promoter

hSyn

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSJX024

Plasmid

#232327

Purpose

Genome editing in Caulobacter crescentus

Depositor

Wei Zhao

Article

Sun et al Nucleic Acids Res. 2025 Apr 22;53(8):gka

Inserts

sgRNA
SpCas9M

Use

CRISPR

Tags

H-arms and cat

Expression

Bacterial

Promoter

PJ23119 and Pxyl

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/TO/V5-DEST-Paxillin-EGFP

Plasmid

#215530

Purpose

Lentiviral transfer vector with EGFP-tagged paxillin

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PXN (PXN Human)

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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MBP-C5E-6XTUBE

Plasmid

#217628

Purpose

Bacterial expression of MBP tagged 6XTUBE

Depositor

Ethan Lee

Article

Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

Insert

6XTUBE

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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A3Bmax

Plasmid

#207167

Purpose

Express full-length human A3B in a BE4max-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)

Tags

NLS and NLS, 6x His

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-E2Crimson

Plasmid

#153425

Purpose

Retroviral (MSCV) expression of E2-Crimson fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

E2-Crimson

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-2xFYVE

Plasmid

#221750

Purpose

Stable expression of GFP-2xFYVE in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

tandem HRS FYVE domain

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

two FYVE domains (amino acids 147-223), linked by…

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)::NL3F10H

Plasmid

#141290

Purpose

NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC3F10H

Use

Luciferase

Tags

Flag (x3) and His (x10)

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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TLCV2-DelGFP

Plasmid

#133302

Purpose

EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.

Depositor

Jingshi Shen

Article

Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NINJ1

Plasmid

#201180

Purpose

Transient expression of NINJ1 gene in mammalian cells

Depositor

Jonathan Kagan

Article

Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

Insert

Ninjurin 1 (Ninj1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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PB_iMYOD1_P2A_BAF60C2_T2A_mCh_Puro

Plasmid

#168807

Purpose

PiggyBac transposon construct with doxycycline inducible MYOD1, BAF60C + mCherry expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

MYOD1 (MYOD1 Human, Synthetic)

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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