We narrowed to 30,244 results for: PLE;

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pCRII-TOPO CMV-cGFP-mMEN b_3' Sense

Plasmid

#46840

Purpose

Express cGFP transcript ending in the wildtype MEN beta triple helix

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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Opto-FFR3

Plasmid

#106065

Purpose

Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-FFR3; Opto-FFR3; F7; 718)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-FFR3

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB2801

Plasmid

#63656

Purpose

pCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCRII-Plxnb3-ISH-2kb

Plasmid

#68037

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B3 (Plxnb3 Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCfB2991

Plasmid

#63643

Purpose

pCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0 miR 19b-3p

Plasmid

#82442

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-19b-3p

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

hsa-miR-19-3p

Mutation

Complementary region for hsa-miR-19-3p as sensor

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' Mut U1/U2 Sense

Plasmid

#46837

Purpose

Express cGFP transcript ending in a mutant MALAT1 triple helix which causes the transcript to be degraded

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pX459-Plxna1 gRNA

Plasmid

#246574

Purpose

CRISPR vector co-expressing Cas9 and a mouse Plxna1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Plxna1 gRNA (Plxna1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTARGEX-CHL

Plasmid

#242447

Purpose

Targets high levels of recombinant protein to chloroplast. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.

Depositor

Allyson MacLean

Article

Chartrand-Pleau et al Front. Synth. Biol. 3:1619871

Type

Empty backbone

Tags

Transit peptide from N. tabacum RbcS

Expression

Plant

Promoter

double 35S

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pTARGEX-APO

Plasmid

#242446

Purpose

Targets high levels of recombinant protein to apoplast. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.

Depositor

Allyson MacLean

Article

Chartrand-Pleau et al Front. Synth. Biol. 3:1619871

Type

Empty backbone

Tags

N-terminal fusion with PR1b signal peptide

Expression

Plant

Promoter

double 35S

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pTARGEX-ER

Plasmid

#242449

Purpose

Targets high levels of recombinant protein for retention in ER. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.

Depositor

Allyson MacLean

Article

Chartrand-Pleau et al Front. Synth. Biol. 3:1619871

Type

Empty backbone

Tags

ER-retrieval motif (KDEL) and Signal peptide deri…

Expression

Plant

Promoter

double 35S

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA046

Plasmid

#240795

Purpose

Plasmid contains the triple selection cassette gfp -pBR322ROP tetApBR322ROP - CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA pBR322ROP His medium : T7- His tags medium- gfp -pBR322ROP tetApBR322ROP - CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA045

Plasmid

#240794

Purpose

Plasmid contains the triple selection cassette gfp -pBR322ROP tetApBR322ROP - CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA pBR322ROP His low : T7- His tags low - gfp -pBR322ROP tetApBR322ROP - CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA043

Plasmid

#240792

Purpose

Plasmid contains the triple selection cassette gfp -p15a tetAp15a - CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA p15a His medium : T7- His tags medium- gfp -p15a tetAp15a - CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA042

Plasmid

#240791

Purpose

Plasmid contains the triple selection cassette gfp -p15a tetAp15a - CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA p15a His low : T7- His tags low - gfp -p15a tetAp15a - CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA040

Plasmid

#240789

Purpose

Plasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 His medium : T7- His tags medium- gfp -RK2 tetARK2 - CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA039

Plasmid

#240788

Purpose

Plasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 His low : T7- His tags low - gfp -RK2 tetARK2 - CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pYO539

Plasmid

#235752

Purpose

Protein expression of ScVPS30 and ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Expression

Yeast

Promoter

GAL-TDH3

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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T7pro-tRNA-ITSIle intron-T7tVar1-pRS426

Plasmid

#239294

Purpose

Expresses the tRNAIle intron bearing the 5' GGGAGA initially transcribed sequence (ITS); Plasmid #2 of the tet-on overexpression system

Depositor

Anita Hopper

Article

Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

Inserts

T7 promoter
ITS-tRNAIle intron
T7 terminator - Var1

Expression

Yeast

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pYO734

Plasmid

#235753

Purpose

Protein expression of ScVPS30 and ZZ-ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Tags

ZZ-3xTEV

Expression

Yeast

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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