We narrowed to 37,735 results for: Ran;

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pLKO.1-puro U6 sgRNA BfuAI stuffer

Plasmid

#50920

Purpose

U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCFJ150 - pDESTttTi5605[R4-R3]

Plasmid

#19329

Depositor

Erik Jorgensen

Article

Christian et al

Insert

pDESTttTi5605[R4-R3]

Expression

Worm

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pT4/HB

Plasmid

#108352

Purpose

Optimized Sleeping Beauty Transposon Vector with MCS

Depositor

Wolfgang Uckert

Article

Wang et al Nucleic Acids Res. 2017 Jan 9;45(1):311-

Type

Empty backbone

Use

Transposon

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-rab11 DN

Plasmid

#12678

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

changed serine 25 to asparagine

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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PKC alpha WT

Plasmid

#21232

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC alpha (PRKCA Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pGL2-p21 promoter-Luc

Plasmid

#33021

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

p21 promoter (CDKN1A Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

p21

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-METTL14

Plasmid

#53740

Purpose

For expression of METTL14 (methyltransferase-like 14) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Methyltransferase-like 14 (METTL14 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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BFP-Sec61 beta

Plasmid

#49154

Purpose

expression of Sec61 beta fused to TagBFP, used as a general ER marker

Depositor

Gia Voeltz

Article

Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

Insert

Sec61 beta (SEC61B Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro p53 DD

Plasmid

#9058

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

p53 DD (Trp53 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

DD. Dominant negative mutant carries deletion of…

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pCAG-GFPd2

Plasmid

#14760

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

EGFPd2

Expression

Mammalian

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pT7-PEmax for IVT

Plasmid

#178113

Purpose

Template for in vitro transcription of PEmax

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Mutation

Detailed in manuscript

Promoter

T7 (inactivated)

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-rab9 WT

Plasmid

#12663

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB9 (Homo sapiens) (RAB9A Human)

Tags

GFP and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pENTR5'_ubi

Plasmid

#27320

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

zebrafish ubiquitin promoter (ubb Zebrafish)

Use

Multisite gateway 5' entry vector

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha-wt-pBabe-puro

Plasmid

#19365

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt human cDNA

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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pTransposon.CAG.CasRX-P2A-Blast

Plasmid

#212965

Purpose

Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.

Depositor

Roland Rad

Article

Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

Insert

CasRx

Use

Transposon

Tags

P2A-Blasticidin resistance

Expression

Mammalian

Promoter

CAGGS

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pORANGE Bsn-GFP KI

Plasmid

#139665

Purpose

Endogenous tagging of Bassoon: C-terminal (amino acid position: F3938)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE Rims1-GFP KI

Plasmid

#131494

Purpose

Endogenous tagging of RIM1: C-terminal (amino acid position: S1615)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Rims1 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE mEos3.2-Camk2a KI

Plasmid

#131493

Purpose

Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and mEos3.2 donor (Camk2a, Camk2a Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-CACNA1A KI

Plasmid

#131480

Purpose

Endogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacna1a, Cacna1a Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI

Plasmid

#116954

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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