We narrowed to 10,414 results for: 158

1. Sth1Cas9n cytosine base editor

   Plasmid

   #194209

   Purpose

   Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording

   Depositor

   Chase Beisel

   Article

   Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

   Insert

   Sth1Cas9-D9A nickase
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Changed Aspartate 9 to Alanine.

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. UPB-pancreas (UPB7)

   Plasmid

   #180787

   Purpose

   Unique Projection Barcode (UPB7)-Pancreas for Projection-seq

   Depositor

   Rui Chang

   Article

   Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

   Insert

   hM4D(Gi) (partial )
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBR322-Pdp1_NTD-Cre-HiBiT

   Plasmid

   #198318

   Purpose

   Loads HiBiT-tagged Cre into PVCs using Pdp1 packaging domain; also contains Pnf

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Pdp1_NTD-Cre-HiBiT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CA-0113 CMVTO-Renin(mouse)-ECL

   Plasmid

   #231200

   Purpose

   Membrane-bound mouse renin

   Depositor

   Xiaojing Gao

   Article

   Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

   Insert

   Renin(mouse)-ECL (Ren1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL3713 (pTnsC_Pse)

   Plasmid

   #200900

   Purpose

   Expresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseTnsC
   

   Use

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJIT163-AFID-3

   Plasmid

   #167359

   Purpose

   For inducing predictable deletions in protoplasts and wheat plants

   Depositor

   Caixia Gao

   Article

   Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

   Insert

   A3A-Cas9-UDG-T2A-AP lyase
   

   Use

   Tags

   Expression

   Plant

   Mutation

   WT

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLS.402

   Plasmid

   #187202

   Purpose

   Expresses retron Eco1 RT

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Insert

   Retron Eco1 Reverse Transcriptase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   mphR

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. enOgeuIscB with ωRNA-v13

   Plasmid

   #220940

   Purpose

   CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v13 scaffold with BsmBI for guide cloing

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

   Inserts

   CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
   OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCherry-N1_SEPT2

   Plasmid

   #71549

   Purpose

   mammalian expression of Sept2 with an mCherry fusion.

   Depositor

   Elias Spiliotis

   Article

   Bowen et al J Cell Biol. 2011 Jul 25;194(2):187-97.

   Insert

   Septin 2 (Septin2 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKanB alpha

    Plasmid

    #223524

    Purpose

    Used for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

    Depositor

    Geoff Lin-Cereghino

    Article

    Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Sept. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA3.1/Zeo-CnB

    Plasmid

    #191965

    Purpose

    Expression of CnB (calcineurin subunit beta).

    Depositor

    Luca Scorrano

    Article

    Cereghetti et al Proc Natl Acad Sci U S A. 2008 Oct 14;10

    Insert

    Calcineurin subunit beta (PPP3CB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA5/FRT-ChemoB-ATP

    Plasmid

    #193819

    Purpose

    CMV overexpression of ChemoB-ATP in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoB-ATP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EBFP2:N39Y, A206K; HT7: L271E

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. IVT-nCas9(H840A)

    Plasmid

    #207455

    Purpose

    in vitro transcription of mRNA

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

    Insert

    H840A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA5/FRT-ChemoR-ATP

    Plasmid

    #193820

    Purpose

    CMV overexpression of ChemR-ATP in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoR-ATP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBMN-FKBP DD(L106P)-UbK0G75S-sfGFP

    Plasmid

    #170157

    Purpose

    Expression for LIBRON constructs

    Depositor

    Thomas Wandless

    Article

    Miyamae et al Cell Chem Biol. 2020 Dec 17;27(12):1573-

    Insert

    FKBP DD (L106P)-UbK0G75S
    

    Use

    Retroviral

    Tags

    sfGFP

    Expression

    Mutation

    change all lysin residues of ubiquitin to Arg, G7…

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Marathon-RT pentamutant for Split-PE (pEH107)

    Plasmid

    #190110

    Purpose

    Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    D14R, N26R, D74R, N116K, N197R

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA5/FRT-ChemoL-CaM

    Plasmid

    #193830

    Purpose

    CMV overexpression of ChemoL-CaM in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoL-CaM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EGFP:A206K, E299D; HT7: L271E

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ND4.2-Left TALE-G1397-N-wildtype

    Plasmid

    #179684

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N_1xUGI_P2A_mCherry_ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRN3P_EGFP_m6A_Tracer

    Plasmid

    #139403

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    m6A-Tracer
    

    Use

    In vitro transcription

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    T3

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMT451

    Plasmid

    #139391

    Purpose

    Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4), pNBU1 backbone, AmpR, ErmR

    Depositor

    Christopher Voigt

    Article

    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

    Insert

    Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    BA inducible promoters

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pNTI822

    Plasmid

    #216631

    Purpose

    Tet-inducible cpTurboID (V5) for Sleeping Beauty transposon system

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    cpTurboID
    

    Use

    Sleeping beauty transposon system

    Tags

    V5

    Expression

    Mammalian

    Mutation

    L120P

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ND4.2-Right DdCBE-G1397-C-wildtype

    Plasmid

    #179687

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C )_1xUGI_SOD2 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. M3D395A-M14-dCas9

    Plasmid

    #134780

    Purpose

    Expression of fusion protein M3D395A-M14-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    METTL3/METTL14 methyltransferase domain and inactive dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D395A

    Promoter

    CMV

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-Cas9-NRCH

    Plasmid

    #136926

    Purpose

    Expresses Cas9-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRCH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 3NES-CS(G)-tTA-CS(G)-3NES

    Plasmid

    #137836

    Purpose

    Soluble tTA transcription factor flanked by N and C terminal TEV protease G cleavage sequences and 3 nuclear export sequences

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    3NES-CD(G)-tTA-CS(G)-3NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p2T-CMV-evoCjCas9-TadCDd-BlastR

    Plasmid

    #202558

    Purpose

    Mammalian expression of evoCjCas9 TadCDd

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-TadCDd
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CJ8421_04975 Rptr2 and CJ8421_04975 mRNA

    Plasmid

    #194210

    Purpose

    Expresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    CJ8421_04975 mRNA and CJ8421_04975 Rptr2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFastBac_human-IL-18

    Plasmid

    #214338

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. tracrRNA(scr)-CJ8421_04975 mRNA

    Plasmid

    #199718

    Purpose

    Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    CJ8421_04975 mRNA and scrambled tracrRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LentiGuide_CjCas9-Puro

    Plasmid

    #202564

    Purpose

    hU6-BsmBI-gRNA-Puro

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    CjCas9 sgRNA cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge