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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,585 results for: Met;

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Showing: 4261 - 4280 of 19585 results

  4. pPB9v2

    Plasmid
    #210029
    Purpose
    Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Article
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    Cas9
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210029-plasmid-map-sequence-id-414001

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  5. scFv-GCN4-DNMT3a-DNMT3l

    Plasmid
    #154140
    Purpose
    Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.
    Depositor
    Albert Jeltsch
    Article
    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i
    Insert
    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    Tags
    HA and sfGFP
    Expression
    Mammalian
    Promoter
    SFFV
    Available Since
    Oct. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    154140-plasmid-map-sequence-id-345614

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  6. MICU-APEX2

    Plasmid
    #79057
    Purpose
    A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    C-terminal APEX2 fused MICU1 (MICU1 Soybean, Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Bacterial and Mammalian
    Promoter
    CMV immearly promotor
    Available Since
    Sept. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    79057-plasmid-map-sequence-id-147904

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  13. MO91-dCas9-AID(P182X)

    Plasmid
    #83260
    Purpose
    Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.
    Depositor
    Xing Chang
    Article
    Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.
    Insert
    dCas9-AID P182X
    Use
    Retroviral
    Tags
    3xFLAG-NLS and FLAG
    Expression
    Mammalian
    Mutation
    D10A and H840A in Cas9; P182X in AID
    Promoter
    MSCV
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV.hSyn.iGluSnFr.WPRE.SV40

    Plasmid
    #98929
    Purpose
    AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    iGluSnFr
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    98929-plasmid-map-sequence-id-190089

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Showing: 4261 - 4280 of 19585 results