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Showing: 4261 - 4280 of 31265 results

  3. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

    Plasmid
    #200910
    Purpose
    3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    3XHA_den1FnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Mutation
    Changed Glutamic Acid to Arginine at 1369 position
    Promoter
    CBH
    Available Since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200910-plasmid-map-sequence-id-407164

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  17. pMSCV-KD-mAbl

    Plasmid
    #69028
    Purpose
    Retorviral vector encoding Kinase Defective mouse c-Abl
    Depositor
    Jean Wang
    Article
    Sridevi et al PLoS One. 2013 Oct 11;8(10):e77495. doi:
    Insert
    mouse c-Abl Type VI - kinase defective
    Use
    Retroviral
    Tags
    FLAG tag inserted at Internal SalI site (2934 bp)
    Expression
    Mammalian
    Mutation
    The KD (kinase defective) mutation is at Lysine 2…
    Available Since
    Sept. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. mutpRL-TK 3xUTR

    Plasmid
    #40758
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    3 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available Since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pOPTXcGMPRELUC

    Plasmid
    #68503
    Purpose
    cyclic nucleotide reporter for bacteria or plant cells
    Depositor
    Helen Irving
    Article
    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1
    Insert
    OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
    Tags
    luciferase
    Expression
    Bacterial and Plant
    Mutation
    3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…
    Promoter
    OPTX promoter with cGMPRE
    Available Since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 4261 - 4280 of 31265 results