167,846 results

1. MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)

   Plasmid

   #160369

   Purpose

   Stable expression of GPR143-mScarlet-3xHA fusion protein for the isolation of melanosomes.

   Depositor

   David Sabatini

   Article

   Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

   Insert

   GPR143 (GPR143 Human)
   

   Use

   Lentiviral

   Tags

   mScarlet-3xHA

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3-EF1a-NICD1

   Plasmid

   #46047

   Depositor

   Xin Chen

   Article

   Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

   Insert

   Notch1 (C-term) (Notch1 Mouse)
   

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Cytoplasmic domain initiating at amino acid 1753

   Promoter

   EF1a

   Available Since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. CD47-bio-His

   Plasmid

   #53150

   Purpose

   Expresses full-length CD47 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   CD47 (CD47 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-FLEx-ER-TurboID

   Plasmid

   #160857

   Purpose

   Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Has Service

   AAV8

   Insert

   ER TurboID
   

   Use

   AAV

   Tags

   HA and V5

   Promoter

   CAG

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJH001

   Plasmid

   #67641

   Purpose

   High copy Cas9 expression vector with LEU2 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   Empty
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-BirA*-dCas9-eGFP

   Plasmid

   #84779

   Purpose

   Expresses BirA*-dCas9-eGFP in mammalian cells.

   Depositor

   Heinrich Leonhardt

   Article

   Schmidtmann et al Nucleus. 2016 Sep 27:0.

   Insert

   BirA*-dCas9-eGFP
   

   Use

   CRISPR

   Tags

   cMyc

   Expression

   Bacterial and Mammalian

   Promoter

   CAG

   Available Since

   July 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pORF2

   Plasmid

   #206026

   Purpose

   An expression vector for mouse LINE1 ORF2

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   ORF2 derived from mouse LINE1
   

   Expression

   Mammalian

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPECS library

   Pooled Library

   #127842

   Purpose

   For performing high-throughput cell state-specific synthetic promoter screening

   Depositor

   Timothy Lu

   Article

   Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

   Species

   Synthetic

   Use

   Lentiviral

   Available Since

   Aug. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

9. pDONR221_HDLBP

   Plasmid

   #154448

   Purpose

   For recombination of HDLBP

   Depositor

   Markus Landthaler

   Article

   Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

   Insert

   HDLBP (HDLBP Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV5)

    Viral Prep

    #26975-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    CaMKII

    Tags

    mCherry

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro

    Plasmid

    #234950

    Purpose

    Human codon optimized Ku (Mycobacterium) with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    human codon optimized Ku with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Anti-GFP [N86/38.1R] (Recombinant Antibody)

    Antibody

    #180084-rAb

    Purpose

    Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody. Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively).

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Recommended Applications

    Immunocytochemistry, Immunohistochemistry, and Western Blot

    Reactivity

    Synthetic

    Source Species

    Mouse

    Isotype

    IgG2a

    Trial Size

    Available to purchase

    Available Since

    March 29, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

13. pLX317-MBNL1

    Plasmid

    #115443

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    MBNL1 (MBNL1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRRLsinPGK_IRFP720_WPRE

    Plasmid

    #91785

    Purpose

    Constitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.

    Depositor

    Beverly Torok-Storb

    Insert

    iRFP720
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. fluoPEER-GFP>BFP

    Plasmid

    #185477

    Purpose

    Fluorescent reporter for a Y66H mutation to convert a GFP to BFP.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    GFP-Y66-editing-site
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmScarlet-i_C1

    Plasmid

    #85044

    Purpose

    Mammalian expression of mScarlet-i

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    mScarlet-i
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNCS-mGold2t

    Plasmid

    #231766

    Purpose

    Expression of mGold2t in bacterial cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2t
    

    Expression

    Bacterial

    Mutation

    mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    Synthetic promoter

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNCS-mGold2s

    Plasmid

    #231765

    Purpose

    Expression of mGold2s in bacterial cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2s
    

    Expression

    Bacterial

    Mutation

    mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    Synthetic promoter

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV enAsCas12f-HKRA-sgRNA_v7

    Plasmid

    #208357

    Purpose

    Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-HKRA
    

    Expression

    Mammalian

    Mutation

    I123H/D195K/D208R/V232A

    Promoter

    CMV

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. CGBE1-NG (pBM1246)

    Plasmid

    #140256

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_NG-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pUltra-Plus-H2B-mTagBFP2

    Plasmid

    #161790

    Purpose

    3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Insert

    H2B-mTagBFP2
    

    Use

    Lentiviral; Bicistronic

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    I174A in the mTagBFP2 protein according to PLoS O…

    Promoter

    UbC and UbC promoter

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pFBOH-MHL_DCAF11:1-546

    Plasmid

    #210907

    Purpose

    Insect expression of full-length human DCAF11. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DCAF11:M1-Q546 (DCAF11 Human)
    

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    R207H natural variant (VAR_020121)

    Promoter

    Polyhedrin

    Available Since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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23. pmCherry-Paxillin

    Plasmid

    #80029

    Purpose

    Mammalian expression of mouse Paxillin

    Depositor

    Chinten James Lim

    Insert

    Paxillin (Pxn Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. OPRM1-DuET

    Plasmid

    #213361

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OPRM1 (OPRM1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pDEST-V1-ORF

    Plasmid

    #73635

    Purpose

    Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Tags

    Venus fragment 1 (V1)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pDONR221-NPC1

    Plasmid

    #132295

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    NPC1 (NPC1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 14, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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27. EcSTH

    Plasmid

    #211923

    Purpose

    This plasmid contains human codon optimized sequence of EcSTH which can be used to increase NADH/NAD ratio and decrease NADPH/NADP ratio in mammalian cells.

    Depositor

    Binghui Li

    Article

    Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

    Insert

    EcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. VPS13A^mCherry

    Plasmid

    #118758

    Purpose

    VPS13A internally tagged with mCherry at residue 1372.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13A (VPS13A Human)
    

    Tags

    mCherry inserted at position 1372

    Expression

    Mammalian

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pJSK664

    Plasmid

    #205130

    Purpose

    Expression of GFP-2xPX (from SnxA) in mammalian cells

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    tandem 2xPX domain of SnxA
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pDEST-V2-ORF

    Plasmid

    #73636

    Purpose

    Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Tags

    Venus fragment 2 (V2)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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