163,421 results
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TOP-GFP.mC
Plasmid#35491DepositorInsert7xTOP-GFP
UseLentiviralTagsExpressionMammalianMutationPromoter7xTCF/LEFAvailable sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSC-humanADAR1
Plasmid#198235PurposeExpresses human ADAR1 in yeastDepositorInserthuman ADAR1 (ADAR Human)
UseTagsHis10_TEVExpressionYeastMutationPromoterGAL1Available sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
E. coli MEV20
Bacterial Strain#197113PurposeE. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette clusterDepositorBacterial ResistanceNoneAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dual sgRNA CRISPRi Library
Pooled Library#1000000192DepositorAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xFLAG-Pontin
Plasmid#51635PurposeMammalian expression of 3xFLAG tagged pontin, N-terminal.DepositorInsertPontin (RUVBL1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AviTag-SnoopLigase
Plasmid#105626PurposePlasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylationDepositorInsertAviTag-SnoopLigase
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4
Plasmid#214137PurposeInsect cell expression of the human 20SCP proteasome assembly chaperonesDepositorInsertsUseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Pol-II WT
Plasmid#35175DepositorInsertRPBI (POLR2A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN4
Plasmid#180285PurposeKanR multi-gene yeast expression vector (GFP-dropout)DepositorInsertConLS' - GFP - ConRE' - KanR - CEN6/ARS4
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
synIRES_RC25
Plasmid#189970PurposePart 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAsDepositorInsertsynIRES_RC25
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Caveolin-C-10
Plasmid#55008PurposeLocalization: Membrane, Excitation: 587, Emission: 610DepositorInsertCaveolin (CAV1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-M13-TEV-C-P2A-TdTomato
Plasmid#92391Purposeproduces AAV expressing Cal-light M13-TevC component and TdTomatoDepositorInsertM13-TEV-C-P2A-TdTomato
UseAAV and Synthetic BiologyTagsTdTomatoExpressionMammalianMutationPromoterhuman synapsinAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Neo DEST (685-3)
Plasmid#17292Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, NeoDepositorTypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
TFORF3482
Plasmid#144958PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX2 (SOX2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
5` CC: Puro – loxP – GFP-C
Plasmid#219563Purpose5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.DepositorInserthPGK-promoter_PuroR_2A_loxP_GFP-C
UseUnspecifiedTagsExpressionMutationPromoterhPGKAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCG-Hc∆18
Plasmid#84817PurposeMeasles virus hemagglutinin expressing plasmidDepositorInsertMeasles virus H glycoprotein
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG_smFP FLAG
Plasmid#59756Purposeencodes multiple copies of an epitope tag FLAGDepositorInsertsmFP _FLAG
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
PKA-KTR-mStrawberry reporter
Plasmid#158688PurposeThis plasmid is a PKA pathway reporter. It has CMV promoter driving the expression of PKA-KTR fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-NLS-I-PpoI
Plasmid#46963PurposeFor constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.DepositorInsertNLS-I-PpoI
UseTagsHAExpressionMammalianMutationPromoterCMV-TETAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSG5-HA-p300
Plasmid#89094Purposemammalian expression of full-length human p300 in pSG5DepositorInsertp300 (EP300 Human)
UseTagsHAExpressionMammalianMutationPromoterSV40Available sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
JNK-KTR-mStrawberry reporter
Plasmid#158689PurposeThis plasmid is a JNK pathway reporter. It has CMV promoter driving the expression of JNK-KTR fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-rDA3m
Plasmid#208702PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neuronsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA3m
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG mito-RCaMP1h
Plasmid#105013Purposemitochondrial calcium sensorDepositorInsertRCaMP1h
UseTagsExpressionMammalianMutationPromoterpCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-tTA
Plasmid#104102PurposeExpresses tTA2 under the CAG promoter in mammalian cells.DepositorInserttTA2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCB578
Plasmid#141095PurposeE. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat arrayDepositorInsertsCas9 and tracrRNA
repeat-spacer-repeat array
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-F30-Tornado-Acyclovir-Broccoli#4
Plasmid#216822PurposeExpresses acyclovir-controllable Broccoli aptamer #4 as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-Acyclovir-Broccoli#4
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBOB-Lck-GFP
Plasmid#118738PurposeTo express membrane targeted GFPDepositorInsertLck domain (LCK Human)
UseLentiviralTagsGFPExpressionMutationH8N (see comments)PromoterCMVAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flpe
Plasmid#13787PurposeMammalian expression of enhanced form of Flp recombinase with C-terminal Myc tagDepositorInsertFlpe
UseTagsMycExpressionMammalianMutationPromoterCAGAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgRosa26
Plasmid#159914PurposeMutagenesis of Rosa26 with SauCas9DepositorInsertRosa26 gRNA (Gt(ROSA)26Sor Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only
