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MiniCoopR U6:gRNA, mitfa:Cas9

Plasmid

#118840

Purpose

Targets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Type

Empty backbone

Use

CRISPR; Tol2

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pZY101

Plasmid

#73932

Purpose

This binary vector, also named PTF101, is used as a standard plant transformation vector. For more information, please see http://plantsci.missouri.edu/muptcf/pzy101.html

Depositor

Zhanyuan Zhang

Article

Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E

Insert

bar

Expression

Plant

Promoter

35S

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pFBP_promoter_acceptor

Plasmid

#189920

Purpose

Binary vector to accept a custom promoter sequence to drive fungal luciferase expression by Agrobacterium-mediated transient transformation

Depositor

Adam Steinbrenner

Article

Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(

Type

Empty backbone

Expression

Plant

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTF101.2

Plasmid

#134771

Purpose

A binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.

Depositor

Kan Wang

Article

Testroet et al In Vitro Cell.Dev.Biol.-Plant (2017) 53:

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDHt-SK

Plasmid

#63615

Purpose

The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pARS3_MUbCAS9_GW

Plasmid

#128646

Purpose

T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNA

Depositor

G. Eric Schaller

Article

Worthen et al Development. 2019 Jun 3. pii: dev.174870

Type

Empty backbone

Tags

HA

Expression

Plant

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pRGEB33

Plasmid

#158151

Purpose

Construction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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PKC beta II CAT

Plasmid

#16384

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain, aa 329-673

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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PEP109E

Plasmid

#97395

Purpose

binary construct of cytosolic mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

cytosol

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRATIO4212

Plasmid

#141313

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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CFP-FKBP

Plasmid

#162433

Purpose

for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

CFP-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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P16ΔS:S10-(MCS)

Plasmid

#108182

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

P16ΔS

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pESC/mCitrine

Plasmid

#117996

Purpose

Expression of mCitrine controlled by the Gal1 promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

mCitrine

Use

Yeast/bacteria binary vector

Expression

Yeast

Promoter

Gal1

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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PEP104E

Plasmid

#97390

Purpose

binary construct of mitochondria-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

mitochondria target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRATIO3267

Plasmid

#141312

Purpose

Gateway cloning compatible dual bioluminescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

gLUC and redLUC

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRATIO3212

Plasmid

#141311

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-miR-29b-1~29a

Plasmid

#51376

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

pri-miR-29b-1~29a (MIR29B1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV hSynapsin flexed SV-tag

Plasmid

#163686

Purpose

express SV-Tag construct in a Cre-dependent manner

Depositor

Bernardo Sabatini

Article

Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

Insert

synaptophysin 9X HA

Use

AAV

Tags

9X HA

Expression

Mammalian

Promoter

synapsin

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:GFP1–10

Plasmid

#125663

Purpose

Binary vector for the inducible expression of GFP1–10 fragment that can be used to detect a protein tagged with GFP11

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

GFP1–10

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRATIO2214

Plasmid

#141303

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mCerulean and mScarlet-I

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R-G324D-(Kin-8)

Plasmid

#28179

Depositor

Bernard Schimmer

Article

Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

Insert

Prkar1a (PRKAR1A Mouse)

Expression

Mammalian

Mutation

G324D

Available Since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
SPDK3181

Plasmid

#97403

Purpose

binary construct of ER-targeted sfCherry1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

ER-sfCherry1-10

Tags

ER target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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P16ΔS:GFP1–9

Plasmid

#108186

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components (GFP1-9) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Insert

GFP1-9

Use

Synthetic Biology

Expression

Plant

Promoter

P16ΔS

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
SPDK3182

Plasmid

#97404

Purpose

binary construct of ER-targeted sfYFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

ER-sfYFP1-10

Tags

ER target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
SPDK3184

Plasmid

#97405

Purpose

binary construct of cytosolic sfCFP1-10 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfCFP1-10

Tags

cytosol

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PEP106E

Plasmid

#97392

Purpose

binary construct of ER-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

ER target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDH

Plasmid

#67584

Purpose

Gene expression in plants. A promoter and/or reporter can be cloned into this plasmid

Depositor

Indu Maiti

Article

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Type

Empty backbone

Expression

Plant

Promoter

None

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYL1300UaUf

Plasmid

#183173

Purpose

Plant binary vector with ccdB flanked by Ua and Uf unique nucleotide sequences.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRATIO1112

Plasmid

#141066

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRATIO1267

Plasmid

#141301

Purpose

Gateway cloning compatible dual bioluminescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

gLUC and redLUC

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRATIO4214

Plasmid

#141314

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mCerulean and mScarlet-I

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PEP102E

Plasmid

#97388

Purpose

binary construct of nuclear-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Nuclear target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
LT0

Plasmid

#210551

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT0::mCherrySSM

Expression

Plant

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LT1

Plasmid

#210552

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT1::mCherrySSM

Expression

Plant

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LT2

Plasmid

#210553

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT2::mCherrySSM

Expression

Plant

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PEP107E

Plasmid

#97393

Purpose

binary construct of Golgi-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Golgi target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PEP114E

Plasmid

#97400

Purpose

binary construct of ER-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

ER target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PEP103E

Plasmid

#97389

Purpose

binary construct of plastid-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Plastid target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PEP105E

Plasmid

#97391

Purpose

binary construct of peroxisome-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Peroxisome target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRATIO2212

Plasmid

#141302

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pC1300_pUB10_pco-vCASphi_E9t_MCS_version2

Plasmid

#197961

Purpose

T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pco-vCasphi-2

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pC1300_pUB10_pco-nCASphi_E9t_MCS_version2

Plasmid

#197962

Purpose

T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pco-nCasphi-2

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PKC beta II dNPS

Plasmid

#16383

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

N-terminal pseudosubstrate domain deletion: aa 30…

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pARS3_MUbCAS9_MC

Plasmid

#128767

Purpose

T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a MCS for cloning gRNA

Depositor

Zachary Nimchuk

Article

Worthen et al Development. 2019 Jun 3. pii: dev.174870

Type

Empty backbone

Tags

HA

Expression

Plant

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R-R334W-(Kin-7)

Plasmid

#28178

Depositor

Bernard Schimmer

Article

Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

Insert

Prkar1a (PRKAR1A Mouse)

Expression

Mammalian

Mutation

R334W

Available Since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pRATIO1212

Plasmid

#141299

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLpC-L-IFP-F[1] (mammalian)

Plasmid

#52901

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[2] (mammalian)

Plasmid

#52902

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pSi

Plasmid

#67884

Purpose

Derivative of pSiM24 plant expression vector without the M24 promoter

Depositor

Indu Maiti

Article

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Type

Empty backbone

Expression

Plant

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pN_35S/mCitrine

Plasmid

#117994

Purpose

Expression of mCitrine controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

mCitrine

Use

Plant/bacteria binary vector

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 10851 - 10900 of 11638 results

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