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Showing: 421 - 440 of 66320 results

  2. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60229
    Purpose
    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    28 Articles
    60229-plasmid-map-sequence-id-419029

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  8. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Publication
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    24 Articles
    73797-plasmid-map-sequence-id-196924

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  14. pAAV-CAG-tdTomato (codon diversified)

    Plasmid
    #59462
    Purpose
    AAV-mediated expression of tdTomato (codon diversified) under the CAG promoter
    Depositor
    Edward Boyden
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.S, AAV PHP.eB, and AAV Retrograde
    Insert
    tdTomato
    Use
    AAV
    Expression
    Mammalian
    Mutation
    codon diversified
    Promoter
    CAG
    Available since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    141 Articles
    59462-plasmid-map-sequence-id-516121

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  16. pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby (AAV1)

    Viral prep
    #71760-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby (#71760). In addition to the viral particles, you will also receive purified pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby plasmid DNA. Syn-driven, Cre-dependent expression of membrane-bound GFP and the presynaptic protein synaptophysin fused to mRuby. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Liqun Luo
    Publication
    Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10
    Tags
    mGFP, mRuby (Cre-dependent)
    Available since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only

  17. pCRIS-PITChv2-dTAG-Puro (BRD4)

    Plasmid
    #91796
    Purpose
    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.
    Depositor
    James Bradner, Behnam Nabet
    Publication
    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d
    Insert
    FKBP_F36V-2xHA-P2A-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Phenylalanine 36 to valine in FKBP12
    Promoter
    Promotorless
    Available since
    March 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    14 Articles
    91796-plasmid-map-sequence-id-334366

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  18. pGP-AAV-syn-jGCaMP8f-WPRE (AAV9)

    Viral prep
    #162376-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-syn-jGCaMP8f-WPRE (#162376). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8f-WPRE plasmid DNA. Syn-driven expression of ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 421 - 440 of 66320 results