We narrowed to 4,533 results for: Gaa

Showing: 421 - 440 of 4533 results

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pk335-CARGO-6mer-30kb-DSF

Plasmid

#227483

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 30kb Downstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-5mer-30kb-DSF

Plasmid

#227484

Purpose

5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 30kb Downstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-25kb-USF

Plasmid

#227468

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 25kb Upstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-7.1kb-USF

Plasmid

#227474

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 7.1kb Upstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-PrPro

Plasmid

#227453

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to Prdm8 promoter Upstream Prdm8

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-40kb-USF

Plasmid

#227460

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 40kb Upstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-11kb-USP

Plasmid

#227447

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 11kb Upstream Prdm8

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCM100_ PB5'IR-hU6-gRNA-CS1- CAG-tdTomato- PB3'IR

Plasmid

#229995

Purpose

PiggyBac sgRNA cloning plasmid with tdTomato reporter with a capture sequence (cs1)

Depositor

Christian Mayer

Article

Dvoretskova et al Nat Neurosci. 2024 May;27(5):862-872. do

Type

Empty backbone

Use

CRISPR

Tags

NotI site for NEBuilder HiFi DNA Assembly with ss…

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGMC00033 (aka pMC0224)

Plasmid

#210750

Purpose

Guide RNA targeting SERPINB3

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

SERBPINB3 guide RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet_Seq1.2

Plasmid

#201404

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgGPAA1 guide 2

Plasmid

#193609

Purpose

GPAA1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgGPAA1 guide 2 (GPAA1 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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LAMP1-RpHLuorin2

Plasmid

#171720

Purpose

Lysosomal expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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Stx5L-mCherry

Plasmid

#209844

Purpose

Expresses Stx5L-mCherry in mammalian cells

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

STX5 (STX5 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Wildtype Stx5 (with 55M)

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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CHGA-eGFP

Plasmid

#228569

Purpose

Expresses GFP-tagged human Chromogranin A in mammalian cells

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

eGFP

Expression

Mammalian

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_TFAP2C_cterm

Plasmid

#222916

Purpose

Cas9/sgRNA plasmid for targeting TFAP2C

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, TFAP2C sgRNA (TFAP2C Human, Synthetic)

Use

CRISPR

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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mEGFP-PI35P2

Plasmid

#92419

Purpose

Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

Depositor

Geert van den Bogaart

Article

Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

Insert

MCOLN1 (Mcoln1 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Synthetic gene, codon optimized for human.

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-HDAC1 ts2

Plasmid

#115848

Purpose

HDAC1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HDAC1 shRNA (HDAC1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLCHKO_HPRT1_exon2_1_Lb

Plasmid

#155051

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA for deletion of HPRT1 exon 2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-RFP-IKZF1-sh2

Plasmid

#69041

Purpose

3rd generation lentiviral vector expressing IKZF1 shRNA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1 (IKZF1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pUDE710

Plasmid

#103020

Purpose

expression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crADE2-3.crHIS4-4.S (ADE2, HIS4 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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