We narrowed to 15,976 results for: LEA
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  Plasmid#216285PurposeExpresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.DepositorInsertDoubleCatcher H-Lock
 TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only
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  pET28a-SnoopTag-AffiHER2-SpyTagPlasmid#216280PurposeExpresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expressionDepositorInsertSnoopTag-AffiHER2-SpyTag
 UseAffinity Reagent/ AntibodyTagsHis6, SnoopTag, SpyTag, and Thrombin cleavage sit…ExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only
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  pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmbPlasmid#175534PurposeExpression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag7-myc_PDGFRtmb
 ExpressionMammalianAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only
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  5UAS zfSynaptophysin:GFPPlasmid#74316PurposeA fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeatsDepositorInsertsynaptophysin
 TagsEGFPMutationQ12H and deletion of F13 (please see depositor co…Promoter5UASAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only
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  pZY101Plasmid#73932PurposeThis binary vector, also named PTF101, is used as a standard plant transformation vector. For more information, please see http://plantsci.missouri.edu/muptcf/pzy101.htmlDepositorInsertbar
 ExpressionPlantPromoter35SAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only
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  FnR9-10Plasmid#182567PurposeHuman fibronectin fragment, type-III repeats 9-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 9-10
 TagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379AAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only
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  pCRISPR-S12Plasmid#84031PurposeTo episomally express codon optimized Cas9 and chimeric guide RNADepositorInsertshSpCas9
 eGFP
 Tags3x FLAGExpressionMammalianPromoterCMV and CMV (downstream of F2A self-cleaving pept…Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only
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  UAS-Syt::Halo7Plasmid#87648PurposeFor creating drosophila transgenics expressing UAS-Syt::Halo7DepositorInsertSyt::Halo7
 ExpressionInsectMutationS265G in Syt (please see depositor's commen…Available SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only
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  KLHL40 (KBTBD5A-c006)Plasmid#98233PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only
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  pcDNA5-H2B_Halo_T2A_EGFPPlasmid#135444PurposeMamalian cell expression of nuclear HaloTag7 and cytosolic EGFPDepositorInsertH2B-HaloTag7 and EGFP
 TagsH2BExpressionMammalianPromoterCMV/TetOAvailable SinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only
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  pEGFP-N1-jGCaMP7b-XNPlasmid#178362PurposeNuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus.DepositorInsertjGCaMP7b-XN
 ExpressionMammalianAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only
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  14UAS zfPSD95:GFPPlasmid#74314PurposeA fusion of zebrafish PSD95 to GFP under control of 14 UAS repeatsDepositorInsertzebrafish PSD95
 TagsEGFPMutationY599H and I793S (please see depositor comment bel…PromoterUASAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only
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  pRGD-TcRPlasmid#74110PurposePlasmid containing tetracycline resistance gene cassette flanked by multiple restriction sitesDepositorInserttetA(C)
 UseBacterial vector based on pmb1 repliconMutationC-to-T transition at pos. no. 1049 of tetA(C) lea…Available SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only
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  pET21a - NRPlasmid#89625PurposeExpresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG inductionDepositorInsertNitrate reductase
 Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only
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  NES-mTagBFP2-P4Mx1Plasmid#108142PurposePI4P biosensorDepositorInsertX.leavis map2k1.L(32-44):mTagBFP2:L. penomophila SidM(546-647)
 ExpressionMammalianPromoterCMVAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only
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  pCMV10-3xFLAG-prs-XKPlasmid#184369PurposeExpress human XK in Expi293 cellsDepositorAvailable SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only
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  Parp14-c011Plasmid#173098PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only
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  TARG1-c002Plasmid#173108PurposeExpression of recombinant fusion protein in E. coliDepositorInsertTARG1
 TagsmCeruleanExpressionBacterialMutationFull length proteinAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only
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  5KO9 (HMCES)Plasmid#101877PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHMCES (HMCES Human)
 Tags6x His (modified C-term tag is AENLYFQSHHHHHH)ExpressionBacterialPromoterT7Available SinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits
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  UtB2N7Plasmid#161015Purposeubiquitously expressed nuclear localized tagBFP2DepositorInserttagBFP2
 ExpressionPlantAvailable SinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only
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  pBA2675Plasmid#189997PurposeInducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)DepositorInsertsFBP75
 VhhGFP4
 UseExpression vector for trypanosoma bruceiTagsNLSMutationN-terminal 600 bp of FBP75Available SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only
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  zCD2Plasmid#122031PurposeExpresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.DepositorInsertZebrafish HDAC6 Catalytic Domain 2
 TagsHis6-MBP tag with cleavable TEV siteExpressionBacterialPromoterT7Available SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only
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  csr-1_son-1_mNeonGreenPlasmid#191743PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
 TagsNCU04502 promoter and mNeonGreenExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only
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  VKG1-gRNA-pX330Plasmid#108671PurposeCleavage of VKG1 sequence by CRISPR/Cas9DepositorInsertgRNA for VKG1 sequence
 UseCRISPRAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only
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  pME-GFP-P2APlasmid#80807PurposeGFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptideDepositorInsertGFP-P2A
 Available SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only
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  pETNb-nALFAPlasmid#159988PurposeFor the protein expression with ALFA tag at N-terminus in E. coliDepositorTypeEmpty backboneTagsMVKYI-8histidine-ALFA tag-HRV3C protease cleavage…ExpressionBacterialAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only
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  pSCW02Plasmid#72301PurposeStarting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.DepositorTypeEmpty backboneUseDerivative of puc19ExpressionBacterialPromoterlacAvailable SinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only
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  pET26b_Nb.AT110.i1Plasmid#135481PurposeE. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptorDepositorInsertNanobody AT110.i1
 Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only
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  pEMS1938Plasmid#82563PurposeHigh copy number plasmid containing Ple261 (CLDN5 MiniPromoter) insert.DepositorInsertPle261
 UsePleiades promoter project [sic, pleaides plieades]Available SinceSept. 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits
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  pDEST14-DoubleCatcher-⍺-LockPlasmid#216286PurposeExpresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ⍺-Lock
 TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only
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  pDEST14-DoubleCatcher-β-LockPlasmid#216287PurposeExpresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher β-Lock
 TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only
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  pDEST14-DoubleCatcher-γ-LockPlasmid#216288PurposeExpresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher γ-Lock
 TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only
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  pDEST14-DoubleCatcher-δ-LockPlasmid#216289PurposeExpresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher δ-Lock
 TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only
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  pDEST14-DoubleCatcher-ε-LockPlasmid#216290PurposeExpresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ε-Lock
 TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only
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  JAK3 (JAK3A-c023)Plasmid#98229PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertJAK3A (JAK3 Human)
 UseBaculovirusTagsHis-6-TEVMutationpfam00069:Pkinase 822-1091PromoterpolyhedrinAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only
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  6CCR (GID4)Plasmid#119960PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
 Tags6xHIS, thrombin,SUMO and TEV cleavageExpressionBacterialMutationcontains amino acids 116-300PromoterT7Available SinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits
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  pECFP-DEVG-VenusPlasmid#24538DepositorInsertECFP-DEVG-Venus
 TagsECFP and VenusExpressionMammalianMutationDEVDR mutated to DEVGPromoterCMVAvailable SinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only
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  pHB-2Plasmid#140955PurposeFor PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.DepositorInsertphaCAB
 ExpressionBacterialMutationR238S mutation in PhaCAvailable SinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only
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  pBABE-puro-NLS-LSSmKate2Plasmid#34586DepositorInsertNLS-LSS-mKate2
 UseRetroviralExpressionMammalianMutationK69Y/P131T/S148G/M167D/T183S/M196V compared to mK…Available SinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only
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  KLHL3 (KLHL3A-c003)Plasmid#110251PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only
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  pEF mito-cGFP (matrix)Plasmid#188904PurposeExpresses mitochondrial GFPDepositorInsertmitochondrial GFP
 TagsMitochondrial leader sequence: MSVLTPLLLRGLTGSARR…ExpressionMammalianAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only
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  pJCC_083 Cas12i1Plasmid#171670PurposeFor bacterial expression of Cas12i1 with an N-terminal His-MBP tagDepositorInsertCas12i1
 Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only
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  pcDNA3-GR-AKAR3Plasmid#173015PurposeImproved green-red FRET-based PKA activity reporter.DepositorInsertGR-AKAR3
 Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only
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  pRDA_449Plasmid#179093Purposenuclease Cas expressionDepositorInsertSpG
 UseCRISPR and LentiviralAvailable SinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only
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  pCMVMagFRET-1-NLSPlasmid#50748PurposeExpresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.DepositorInsertgene encoding for MagFRET-1 with NLS
 ExpressionMammalianAvailable SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only
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  pAD6Plasmid#134349PurposeE. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tagDepositorAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only
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  pET26b_Nb.AT110Plasmid#135480PurposeE. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptorDepositorInsertNanobody AT110
 Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only
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  KEAP1 (KEAP1A-c029)Plasmid#110247PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only
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  pFBNb-cALFAPlasmid#159990PurposeFor the protein expression with ALFA tag at C-terminus in insect cellsDepositorTypeEmpty backboneTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionInsectAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only
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  pCDNA-FRT-FAK30Plasmid#59121PurposeFAK fret sensor with 30 nm helixDepositorInsertFocal Adhesion Kinase
 TagsFLAG, mCerulean, and mCitrineExpressionMammalianPromoterCMVAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only
 
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
      