178,377 results
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Plasmid#140050PurposeExpresses mCherry-tagged HRS 2xFYVE in mammalian cellsDepositorInserttandem HRS FYVE domain (Hgs Mouse)
TagsmCherryExpressionMammalianMutationtwo FYVE domains (amino acids 147-223), linked by…PromoterCMVAvailable SinceMay 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-CBE N-terminal
Plasmid#137175PurposeAAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE N-terminal; U6-protospacer
UseAAVMutationCas9 D10APromoterCbhAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMAL-CellTag-V1 (Concentrated Lentiviral Prep)
Viral Prep#115643-LVCPurposeReady-to-use Concentrated Lentiviral Prep particles produced from pSMAL-CellTag-V1 (#115643). In addition to the viral particles, you will also receive purified pSMAL-CellTag-V1 plasmid DNA. <p><p>Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics.</p></p>DepositorPromoterMinimal CMVTagsEGFPAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-APEX2-C Cloning Vector
Plasmid#128146PurposeTo express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEMDepositorTypeEmpty backboneTagsFlag-APEX2ExpressionMammalianAvailable SinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)
Plasmid#107942PurposeMammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutionsDepositorInserthuman codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
Tags3x HA and NLS (nucleoplasmin)ExpressionMammalianMutationE174R, S542R, K548R, and N282APromoterCAGAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1039
Plasmid#119239Purposeexpresses Tn7 transposaseDepositorInserttnsABCD
ExpressionBacterialAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKM464 (deletions)
Plasmid#108322PurposeOrbit integrating plasmid to delete target gene.DepositorInsertBxb1 attB site
UseOrbit integration plasmid for creating knockouts …Available SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRE4-Azi
Plasmid#105829PurposePlasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cellsDepositorInsertsHumanized EAziRS
4 copies of the U6-BstYam tRNA cassette
ExpressionMammalianMutation(Y37L, D182S, F183M, L186A, D265R)TyrRSPromoterPGK and U6Available SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKII-ArchT-GFP (PV2527) (AAV5)
Viral Prep#99039-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CamKII-ArchT-GFP (PV2527) (#99039). In addition to the viral particles, you will also receive purified pAAV-CamKII-ArchT-GFP (PV2527) plasmid DNA. CaMKII-driven ArchT-GFP expression for optogenetic inhibition.DepositorPromoterCaMKIITagsGFPAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
PPRE-H2B-eGFP
Plasmid#84393PurposeFluorescent reporter for PPARgamma activityDepositorAvailable SinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
3xnls-mScarlet-I
Plasmid#98816PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorInsert3xNLS
TagsmScarlet-IExpressionMammalianPromoterCMVAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Sec61b-C1
Plasmid#90994PurposeExpresses mCherry-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cellsDepositorInsertmCherry-Sec61b (SEC61B Synthetic, Human)
TagsmCherry (red fluorescence)ExpressionMammalianPromoterCMVAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Talin-TS
Plasmid#83376PurposeFRET probe for measuring tension on Talin moleculeDepositorInsertTalin1 (Tln1 Mouse)
UseRetroviralTagsEGFP-F40-tagRFP (tension sensor module)ExpressionMammalianMutationTension sensor module is inserted between V447 an…PromoterCMVAvailable SinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-CMV-VC3AI
Plasmid#78907PurposeIndicator of caspase-3-like protease activityDepositorInsertVC3AI
UseLentiviralTagsHA and MycMutationS320A, deletion of AA1-200PromoterCMVAvailable SinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-IDH1-R132H
Plasmid#62907PurposeExpresses R132 mutant IDH1 in mammalian cellsDepositorAvailable SinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGRB
Plasmid#71539Purposefor expression of gRNA in E. coliDepositorTypeEmpty backboneExpressionBacterialPromoterJ231196Available SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ153
Plasmid#69304PurposeGateway entry vector with pco-dCas9-3X(SRDX)DepositorInsertpco-dCas9-3X(SRDX) (Plant codon-optimized)
UseCRISPRTags2XFLAGMutationSRDX (X3) repressor fusion; NLS fusionAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialPromoterT7Available SinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag C/EBPa
Plasmid#66978PurposeExpression plasmid for mouse C/EBPa; adds Flag tag to the N-terminus of proteinDepositorAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-IRES
Plasmid#64784PurposeLuciferase reporter vectorDepositorTypeEmpty backboneUseLuciferaseAvailable SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only