We narrowed to 11,762 results for: pcDNA

1. pcDNA3.1-MYO10-HMM-Nanotrap

   Plasmid

   #87255

   Purpose

   Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYO10 (Myo10 Mouse)
   

   Use

   Tags

   GFP-nanotrap

   Expression

   Mammalian

   Mutation

   Myo10 is truncated to contain aa.1-941

   Promoter

   CMV

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO-AT1R-APEX

   Plasmid

   #96847

   Purpose

   For expression of AT1R-APEX in mammalian cells

   Depositor

   Andrew Kruse

   Article

   Paek et al Cell. 2017 Apr 6;169(2):338-349.e11. doi

   Insert

   AT1R-APEX (AGTR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT-TO-EGFP-AID

   Plasmid

   #80075

   Purpose

   Mammalian expression vector for N-terminal EGFP-AID tagging

   Depositor

   Andrew Holland

   Insert

   EGFP-AID
   

   Use

   Tags

   AID and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-miR-21 toehold switch

   Plasmid

   #184756

   Purpose

   Expresses miR-21 sensor with RFP output

   Depositor

   Allen Liu

   Article

   Wang et al ACS Synth Biol. 2019 May 17;8(5):1079-10

   Insert

   Red Fluorescence Protein
   

   Use

   Tags

   miR-21 toehold switch

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. integrin beta6 pcDNA1 neo

   Plasmid

   #13580

   Depositor

   Dean Sheppard

   Article

   Weinacker et al J Biol Chem. 1994 Mar 4. 269(9):6940-8.

   Insert

   integrin beta 6 (ITGB6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT-Flag-NRBP1

   Plasmid

   #48197

   Purpose

   Expressed flag-tagged NRBP1 (nuclear receptor binding protein 1)

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

   Insert

   NRBP1 (NRBP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pCDNA3 Flag MKK3b(Glu)

   Plasmid

   #50449

   Purpose

   activates p38 in the signaling pathway

   Depositor

   Roger Davis

   Article

   Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

   Insert

   MKK3b (MAP2K3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Serine 218 to Glutamic Acid, changed Thre…

   Promoter

   CMV

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCDNA-myc-HisA-Sirt3

   Plasmid

   #24924

   Depositor

   Toren Finkel

   Article

   Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

   Insert

   Sirt3 (SIRT3 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-Lyn-RAB-EKARev

   Plasmid

   #118477

   Purpose

   ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   Lyn-RAB-EKARev
   

   Use

   Tags

   N-terminal targeting sequence from Lyn-kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5_NLS-Halo-eGFP-BioID

    Plasmid

    #218448

    Purpose

    expresses the NLS-Halo-eGFP-BioID fusion protein via stable Flp-In integration

    Depositor

    Thorsten Stafforst

    Article

    Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

    Insert

    NLS-Halo-eGFP-BirA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO-GAlphaoB-RLuc8

    Plasmid

    #140977

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaoB-Rluc8 (GNAO1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-CMV-mCherry-hPMCA2x/b

    Plasmid

    #111571

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2x/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3-HA-Akt1-aa120-433

    Plasmid

    #73411

    Purpose

    Expresses HA tagged aa120-433 AKT1

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Expresses aa 120-433 of AKT1

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3 Flag-MBD1-14-mVenusC

    Plasmid

    #191393

    Purpose

    MBD domain of MBD1 fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    pcDNA3 Flag-MBD1-14-mVenusC
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1479 pcDNA3 flag HA 14-3-3 gamma

    Plasmid

    #9000

    Depositor

    William Sellers

    Insert

    14-3-3 gamma (Ywhag Rat)
    

    Use

    Tags

    HA and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1+ GEMIN6 myc HIS

    Plasmid

    #71659

    Purpose

    Mammalian expression of GEMIN6

    Depositor

    Gideon Dreyfuss

    Insert

    GEMIN6 (GEMIN6 Human)
    

    Use

    Tags

    HIS and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-MTS-CA-c-Src-FLAG

    Plasmid

    #44654

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Use

    Tags

    FLAG tag and mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    changed Tyr 530 to Phe

    Promoter

    CMV promoter

    Available since

    May 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-AU1-mTOR-L1460P

    Plasmid

    #19995

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    mammalian target of rapamycin-L1460P (Mtor Rat)
    

    Use

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    Changed Leucine 1460 to Proline

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1-STIM1-Venus-173-C

    Plasmid

    #87619

    Purpose

    Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    STIM1-Venus173-C (STIM1 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-FRT/TO-FLAG-SHLD1

    Plasmid

    #114115

    Purpose

    Expresses N-terminally tagged FLAG-SHLD1 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD1 (SHLD1 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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