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pMSCVpuro-Cre

Plasmid

#34564

Purpose

Retroviral expression of Cre

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Cre

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only

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CMVTO-TVMVP

Plasmid

#179659

Purpose

Tobacco Vein Mottling Viral Protease

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

TVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pERB264

Plasmid

#67764

Purpose

Tags peroxisomes with GFP and Halotag

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

PEX3-GFP-Halo

Tags

GFP and Halotag

Expression

Mammalian

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pQE-WT-T3-R5-RRVH

Plasmid

#63623

Purpose

T3-R5-RRVH RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

T3 RRVH RNAP

Mutation

T745K, N748D, L749M, M750I, G753R, H772R, E775V, …

Promoter

T5/Lac

Available Since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pNL-EGFP/TRETightdU3

Plasmid

#18660

Depositor

Jakob Reiser

Article

Pluta et al J Gene Med. 2005 Jun . 7(6):803-17.

Insert

EGFP

Use

Lentiviral

Available Since

July 8, 2008

Availability

Academic Institutions and Nonprofits only

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pAc-Neo-OVA

Plasmid

#22533

Depositor

Michael Bevan

Article

Moore et al Cell. 1988 Sep 9. 54(6):777-85.

Insert

OVA (OVAL Chicken)

Expression

Mammalian

Available Since

Dec. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_Melt-37-mCherry

Plasmid

#234060

Purpose

Encodes a Melt variant with a switching temperature of 37°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

Melt-37-mCh

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMEV4

Plasmid

#110539

Purpose

The plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.

Depositor

William B. Whitman

Article

Lyu et al ACS Synth Biol. 2016 Jul 15;5(7):577-81.

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MSCV-CMV-DsRed-IRES-d1EGFP

Plasmid

#41942

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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pNL-EGFP/TREPittdU3

Plasmid

#18659

Depositor

Jakob Reiser

Article

Pluta et al J Gene Med. 2005 Jun . 7(6):803-17.

Insert

EGFP

Use

Lentiviral

Available Since

July 8, 2008

Availability

Academic Institutions and Nonprofits only

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pLS416

Plasmid

#239127

Purpose

Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3

Depositor

Sahand Jamal Rahi

Article

Scutteri et al Synth Biol (Oxf). 2025 May 21;10(1):ysaf

Type

Empty backbone

Use

Centromeric

Expression

Bacterial and Yeast

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-OTI-TCRA

Plasmid

#131035

Purpose

Expresses OT-I TCRA

Depositor

Jeff Hammerbacher

Article

Aksoy et al bioRxiv

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV/T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDM291-tetR'-tup11Δ70

Plasmid

#41028

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetR'-tup11Δ70

Expression

Yeast

Mutation

tup11 starts at aa70

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2-NXE+mem-KillerRed

Plasmid

#45761

Depositor

Michael Levin

Article

Jewhurst et al J Cell Death. 2014 Oct 13;7:25-31. doi:

Insert

Membrane-bound KillerRed

Use

Xenopus expression

Promoter

CMV IE94

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-OTI-TCRB

Plasmid

#131036

Purpose

Expresses OT-I TCRB

Depositor

Jeff Hammerbacher

Article

Aksoy et al bioRxiv

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV/T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCDF_LuxR

Plasmid

#140039

Purpose

Constutitive expression of LuxR protein.

Depositor

Yolanda Schaerli

Article

Barbier et al Mol Syst Biol. 2020 Jun;16(6):e9361. doi

Insert

pLuxL and LuxR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d24EGFP

Plasmid

#41944

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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Human-Dysferlin-Original

Plasmid

#60216

Purpose

Human dysferlin (accession #NM_003494) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.

Depositor

Jain Foundation

Insert

Dysferlin (DYSF Human)

Use

Gateway Cloning

Available Since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pUC57-Simple-gRNA backbone

Plasmid

#51306

Purpose

gRNA expression plasmid

Depositor

Yonglong Chen

Article

Guo et al Development. 2014 Jan 8.

Type

Empty backbone

Use

CRISPR; Xenopus expression

Promoter

T7

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCMX-Gal4-TEAD3

Plasmid

#33106

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD3 (TEAD3 Human)

Tags

Gal4

Expression

Mammalian

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pEZYegfp

Plasmid

#18671

Purpose

pEGFPC4 converted Gateway Destination vector for expressing EGFP tagged fusion proteins in mammalian cells

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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pTR47m4-GFP

Plasmid

#102436

Purpose

Formaldehyde biosensor plasmid with sGFP reporter and modified frm promoter

Depositor

Gregory Stephanopoulos

Article

Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

Inserts

Formaldehyde repressor protein (frmR)
Superfolder GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_CMV_Melt-30-mCherry

Plasmid

#234061

Purpose

Encodes a Melt variant with a switching temperature of 30°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

Melt-30-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT mCherry-CaaX

Plasmid

#126669

Purpose

Inducible expression of mCherry-CaaX in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

mCherry-CaaX

Expression

Insect

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pJSH-B14

Plasmid

#20145

Depositor

Glen Hartman

Article

Haudenshield et al Plant Dis. 2011 Mar;95(3):343-352. doi:

Insert

Artificial sequence

Use

Cre/Lox

Mutation

n/a

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pXeX-SHH

Plasmid

#31233

Depositor

Cliff Ragsdale

Article

Agarwala et al Science. 2001 Mar 16. 291(5511):2147-50.

Insert

SHH (SHH Chicken)

Use

Xenopus expression vector

Mutation

starts at aa4

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pRSII41N

Plasmid

#194524

Purpose

Low copy vector backbone with nourseothricin selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII42N

Plasmid

#194525

Purpose

High copy vector backbone with nourseothricin selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MMTV-SV40-Bssk

Plasmid

#1824

Depositor

Philip Leder

Article

Ornitz et al Proc Natl Acad Sci U S A 1991 Feb 1;88(3

Type

Empty backbone

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-mCherry-24xMBS

Plasmid

#86242

Purpose

Middle entry vector for generating MBS tagged RNAs with a fluorescent protein reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

mCherry-24xMBS

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pERB254

Plasmid

#67762

Purpose

Tags mitochondria with Halotag and GFP

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

Halo-GFP-Mito

Tags

GFP and Halotag

Expression

Mammalian

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-GFP

Plasmid

#87662

Purpose

retroviral vector encoding cre dependent expression of GFP

Depositor

Oscar Marin

Article

Ciceri et al Nat Neurosci. 2013 Sep;16(9):1199-210. d

Insert

eGFP

Use

Retroviral

Promoter

CAG

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-CMV-DsRed-IRES-d4EGFP

Plasmid

#41943

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII41K

Plasmid

#194522

Purpose

Low copy vector backbone with G418 selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Tg(mpeg1:EGFP-Mmu.Rpl10a)

Plasmid

#128840

Purpose

RNA isolation from machrophages via TRAP

Depositor

Anna Huttenlocher

Article

Lam et al PLoS One. 2013 Dec 19;8(12):e84436. doi:

Insert

EGFP-L10a

Use

Zebrafish expression

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER-DsRed

Plasmid

#42053

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

DsRed shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pKA-141

Plasmid

#79832

Purpose

Expresses RpBphP1-mCherry

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

BphP1-mCherry

Expression

Mammalian

Promoter

CMVd1

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-kanMX-tetO-Pcyc1

Plasmid

#41023

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetO7-Pcyc1

Use

Promoter replacement

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-Pcdh19-GFP

Plasmid

#35675

Depositor

James Jontes

Article

Biswas et al J Cell Biol. 2010 Nov 29;191(5):1029-41.

Insert

Pcdh19 (pcdh19 Zebrafish)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
PKC eta DN

Plasmid

#21246

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC eta (Prkch Mouse)

Tags

HA

Expression

Mammalian

Mutation

K384R

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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