We narrowed to 23,478 results for: CRISPR

1. pOTX1-donor

   Plasmid

   #112364

   Purpose

   CRISPR donor plasmid to tag human transcription factor OTX1 with GFP

   Depositor

   Kevin White

   Insert

   OTX1 homology arms flanking EGFP-IRES-Neo cassette (OTX1 Human)
   

   Use

   CRISPR

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZNF175-donor

   Plasmid

   #112347

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF175 with GFP

   Depositor

   Kevin White

   Insert

   ZNF175 homology arms flanking EGFP-IRES-Neo cassette (ZNF175 Human)
   

   Use

   CRISPR

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZNF354C-donor

   Plasmid

   #112339

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF354C with GFP

   Depositor

   Kevin White

   Insert

   ZNF354C homology arms flanking EGFP-IRES-Neo cassette (ZNF354C Human)
   

   Use

   CRISPR

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZNF347-donor

   Plasmid

   #112326

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF347 with GFP

   Depositor

   Kevin White

   Insert

   ZNF347 homology arms flanking EGFP-IRES-Neo cassette (ZNF347 Human)
   

   Use

   CRISPR

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

   Plasmid

   #138315

   Purpose

   Lenti expression of mTgBFP2

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   mTAGBFP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. vHB8

   Plasmid

   #193454

   Purpose

   Expression of GFP-Cas9-NLS and gRNA in A. castellanii

   Depositor

   Chantal Abergel

   Article

   Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

   Insert

   GFP-Cas9-NLS
   

   Use

   CRISPR

   Tags

   GFP

   Promoter

   GAPDH

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRU41

   Plasmid

   #167663

   Purpose

   ENTRY vector for gRNA1 cloning under pAtU6 promoter

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pAtU6
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRU42

   Plasmid

   #167664

   Purpose

   ENTRY vector for gRNA2 cloning under pAtU3 promoter

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pAtU3
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRU43

   Plasmid

   #167665

   Purpose

   ENTRY vector for gRNA3 cloning under pAtU6 promoter

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pAtU6
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHFS:SEP-rGluA2 TKIT donor

    Plasmid

    #169440

    Purpose

    Donor DNA for TKIT knockin of SEP to GluA2 in rat

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiGuide-TagRFP-2A-BSD

    Plasmid

    #167930

    Purpose

    Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9

    Plasmid

    #167547

    Purpose

    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Inserts

    chloramphenicol acetyl transferase
    pUC19 origin of replication
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYZ463

    Plasmid

    #187971

    Purpose

    TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-SEP-GluA2 TKIT

    Plasmid

    #169442

    Purpose

    Expression of 2 guides + donor DNA

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    AAV and CRISPR

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pADH110

    Plasmid

    #90982

    Purpose

    NAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Inserts

    NAT 2 of 2
    pSNR52
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBA559

    Plasmid

    #186235

    Purpose

    pTet-LbuCas13a crRNA entry vector

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX856

    Plasmid

    #62888

    Purpose

    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS, NLS SV40, and VP64

    Expression

    Mammalian

    Mutation

    Asparagine 863 to Alanine (N863A)

    Promoter

    CBh

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMiniT:SEP-GluN2A TKIT donor

    Plasmid

    #169429

    Purpose

    Donor DNA for TKIT knockin of SEP to NR2A in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMiniT:SEP-GluN2B TKIT donor

    Plasmid

    #169431

    Purpose

    Donor DNA for TKIT knockin of SEP to NR2B in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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