We narrowed to 89,566 results for: Mal

1. pDEST-N2H-C2

   Plasmid

   #125559

   Purpose

   Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2

   Depositor

   Marc Vidal

   Article

   Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

   Type

   Empty backbone

   Use

   In vitro expression

   Tags

   NanoLuc fragment2 attached to c-terminus of Gatew…

   Expression

   Mammalian and Yeast

   Mutation

   Promoter

   synthetic promoter containing yeast, mammalian, a…

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Circular 200,100 (mIDUA)

   Plasmid

   #170123

   Purpose

   AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (Idua Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and mouse U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLgw Cbx1-V5-EcoDam

   Plasmid

   #59211

   Purpose

   Mammalian DamID positive control lentiviral plasmid. Expresses Cbx1-V5-Dam.

   Depositor

   Bas van Steensel

   Article

   Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

   Insert

   Cbx1 (Cbx1 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   Dam and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Heat Shock Minimal Promoter and heat shock minima…

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-N2H-N2

   Plasmid

   #125548

   Purpose

   Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2

   Depositor

   Marc Vidal

   Article

   Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

   Type

   Empty backbone

   Use

   In vitro expression

   Tags

   NanoLuc fragment2 attached to n-terminus of Gatew…

   Expression

   Mammalian and Yeast

   Mutation

   Promoter

   synthetic promoter containing yeast, mammalian, a…

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. STT3A pEGFP-N2 (1051)

   Plasmid

   #62025

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   STT3A (STT3A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

6. Circular 200,100 with 8 bp bulge loops (GAPDH)

   Plasmid

   #170121

   Purpose

   AAV vector carrying a guide RNA targeting the human GAPDH mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. cacnb4 (rat) in pMT2 vector

   Plasmid

   #107426

   Purpose

   Expresses Calcium channel beta4 auxillary subunit in mammalian cells

   Depositor

   Annette Dolphin

   Article

   Brodbeck et al J Biol Chem. 2002 Mar 8;277(10):7684-93.

   Insert

   cacnb4 (Cacnb4 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ad MLP

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-Flag-FBW7K404R

   Plasmid

   #200717

   Purpose

   The plasmid expresses Flag-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for mammalian overexpression and detection by western blotting.

   Depositor

   Omar Khan

   Article

   Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

   Insert

   FBW7 (FBXW7 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   FBW7 K404 is mutated to Alanine.

   Promoter

   CMV

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-PHFF-sh-mBVRA

   Plasmid

   #100286

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Inserts

   MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
   shRNA for mouse BVRA
   

   Use

   Tags

   FLAG, T7 and Mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   All genes are codon-optimized for expression in h…

   Promoter

   CAG promoter and H1 promoter

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX304-BCL-XL-Y195F

    Plasmid

    #203573

    Purpose

    Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

    Depositor

    Abhishek Chakraborty

    Article

    Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    V5-tagged

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 195 to Phenylalanine for partial…

    Promoter

    CMV

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLENTI_Hu_ECT2(WT)

    Plasmid

    #136330

    Purpose

    Lentiviral expression of human ECT2 WT

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

    Insert

    ECT2 (ECT2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABE-hygro-2xMyc-Rac1-Q61L

    Plasmid

    #128582

    Purpose

    For mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

    Depositor

    Julian Downward

    Article

    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

    Insert

    Rac1 (Rac1 Mouse)
    

    Use

    Retroviral

    Tags

    2xMyc epitope

    Expression

    Mammalian

    Mutation

    Changed Glutamine61 to Leucine.

    Promoter

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. mHDAC7-Spliced (1-938) pEF6-V5 (MJS_014) [Edit]

    Plasmid

    #73267

    Purpose

    Expression of mouse HDAC7-S (1-938) in mammalian cells (V5 tag)

    Depositor

    Matthew Sweet

    Article

    Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

    Insert

    HDAC7 (Hdac7 Mouse)
    

    Use

    Tags

    His/V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. mHDAC7-Spliced (1-938) pEF6-Flag (MJS_015)

    Plasmid

    #73268

    Purpose

    [Edit] Expression of mouse HDAC7-S (1-938) in mammalian cells (Flag tag)

    Depositor

    Matthew Sweet

    Article

    Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

    Insert

    HDAC7 (Hdac7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Fgf4enhLV (FpG12)

    Plasmid

    #69444

    Purpose

    positive control plasmid for lentiviral reporter expression in ESCs

    Depositor

    Lisa Dailey

    Article

    Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

    Inserts

    minimal FGF4 promoter
    EGFP
    Ubiquitin promoter
    Hygromycin resistance
    FGF4 enhancer (Fgf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK297 (DHC1-mAID Hygro)

    Plasmid

    #140542

    Purpose

    DHC1 tagging with mAID

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBactin-Akna-mEGFP

    Plasmid

    #196866

    Purpose

    Expression of the centrosomal protein Akna fused to enhanced (E)-GFP

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Akna-mEGFP (Akna Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX-FKBP-Kras-Blasticidin

    Plasmid

    #120714

    Purpose

    Expresses membrane-targeted recruiter protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    KRAS4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 158-188

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJHG003

    Plasmid

    #126454

    Purpose

    Expresses Cas9-XTEN-dTdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-XTEN-dTdT (DNTT Streptococcus pyogenes, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Added D343E and D345E to inactivate TdT.

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK296 (DHC1-mAID Neo)

    Plasmid

    #140541

    Purpose

    DHC1 tagging with mAID

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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