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pVD1 Multiplexing Edit E3-E4-En-1 (GB2244)

Plasmid

#160566

Purpose

GBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

Multiplexing Edit (E3-E4-En-1)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3 2 kb prom. and exon 1 fragment CD274

Plasmid

#107007

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_4XCoordinatorMutant#1-2-3-4

Plasmid

#173992

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motifs #1, #2, #3 and #4 mutated

Use

Luciferase

Mutation

4X Coordinator motif mutations #1-2-3-4

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-P.1-AVI

Plasmid

#176429

Purpose

Expresses SARS-CoV-2 NTD domain from the P.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-P.1 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

L18F, T20N, P26S, D138Y, R190S

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-P.1-AVI

Plasmid

#176327

Purpose

Expresses SARS-CoV-2 S2P protein from the P.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-S2P-P.1 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-BJ.1-AVI

Plasmid

#196617

Purpose

Expresses SARS-CoV-2 RBD protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-BJ.1

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

G339H, R346T, L368I, S371F, S373P, S375F, T376A, …

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-AY.1-AVI

Plasmid

#176437

Purpose

Expresses SARS-CoV-2 NTD domain from the AY.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-AY.1 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T19R, T95I, G142D, del156-157, R158G, W258L

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-AY.1-AVI

Plasmid

#176335

Purpose

Expresses SARS-CoV-2 S2P protein from the AY.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-S2P-AY.1 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T19R, T95I, G142D, del156-157, R158G, W258L, K417…

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-BJ.1-AVI

Plasmid

#196620

Purpose

Expresses SARS-CoV-2 S2P protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-S2P-BJ.1 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBP-split-intein-Gal4[1-20]-N-int

Plasmid

#199201

Purpose

Gateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expression

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[1-20]-N-int

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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PB513B-1/TRE-hHNF1β-hHNF4α-hHNF6-EF1α-Bla

Plasmid

#199551

Purpose

PiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1β-hHNF4α-hHNF6) under control of the TRE/PCMVmin promoter

Depositor

Masamichi Kamihira

Article

Kitano et al Cells. 2022 Apr 1;11(7):1194. doi: 10.33

Insert

hHNF1β-hHNF4α-hHNF6 (ONECUT1, HNF4A, HNF1B Human)

Use

Piggybac transposon vector

Expression

Mammalian

Promoter

TRE+CMVmin promoter

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-polar-bear-Caspase-1/4b

Plasmid

#183370

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4b

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-harbour-seal-Caspase-1/4b

Plasmid

#183368

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4b

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV:: PDGFR-Cry2olig-FRB-P2A-GFP(1-10)

Plasmid

#221776

Purpose

Expresses PDGFR-Cry2olig-FRB-P2A-GFP(1-10) component in mammalian cells

Depositor

Dongmin Lee

Article

Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

Insert

pCMV:: PDGFR-Cry2olig-FRB-P2A-GFP(1-10)

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry

Plasmid

#124494

Purpose

Expresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherry

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Caspase-1 cleavage seq) T2A mCherry

Use

Synthetic Biology

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTub-split-intein-Gal4[1-20]-N-int

Plasmid

#199210

Purpose

For ubiquitous expression of split-intein Gal4-N-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein Gal4[1-20]-N-int

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-GFP ASXL1 (1-479) 3x FLAG

Plasmid

#81023

Purpose

Cancer-associated ASXL1 truncation mutant in retroviral vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

Contains ASXL1 amino acids 1-479

Promoter

5’LTR

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
pDMJ2-SARS-CoV-2-Spike (B.1-4b)

Plasmid

#241044

Purpose

Expresses SARS-CoV-2 spike (B.1-4b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S494R,K529L,D614G,R683L

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4c)

Plasmid

#241045

Purpose

Expresses SARS-CoV-2 spike (B.1-4c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

N439R,T478K,E484V,D614G,K529L

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4a)

Plasmid

#241042

Purpose

Expresses SARS-CoV-2 spike (B.1-4a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S494R,F490R,D614G,R683N

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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