We narrowed to 48,883 results for: des.1

1. pGEX-6P-1-HsTNRC6A_1209-1709-CIM1-2mut-F1359A_S

   Plasmid

   #147522

   Purpose

   Bacterial Expression of HsTNRC6A_1209-1709-CIM1-2mut-F1359A

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

   Insert

   HsTNRC6A_1209-1709-CIM1-2mut-F1359A (TNRC6A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   one non silent mutation K1578E compared to the se…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-6P-1-HsTNRC6A_1209-1709-M2WA-CIM1-2mut_S

   Plasmid

   #147527

   Purpose

   Bacterial Expression of HsTNRC6A_1209-1709-M2W1-CIM1-2mut

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

   Insert

   HsTNRC6A_1209-1709-M2W1-CIM1-2mut (TNRC6A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   one non silent mutation K1578E compared to the se…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR3-vsv-CenpB 1-166-dCEN-Incenp-GFP

   Plasmid

   #108486

   Purpose

   inducible expression of vsv-Mis12-dCEN-INCENP-EGFP

   Depositor

   Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   CenpB-dCEN-INCENP (INCENP Human)
   

   Use

   Tags

   EGFP and VSV

   Expression

   Mammalian

   Mutation

   CEN box replaced by CenpB 1-166

   Promoter

   CMV

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV CMV-driven AcrIIA4-2xmiR-1 target sites

   Plasmid

   #120299

   Purpose

   AAV vector for expression of AcrIIA4 with two miR-1 binding sites

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIA4-2xmiR-1 binding sites
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

   Plasmid

   #85111

   Purpose

   expression of recombinant human mature (delta19) ETFalpha wt without tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta19) ETFalpha (ETFA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

   Plasmid

   #138525

   Purpose

   Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

   Depositor

   Richard Jenner

   Article

   Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

   Insert

   Control non-PRC2 binding A-tract repeat RNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

   Plasmid

   #58275

   Purpose

   mammalian expression of human RdgBbeta splice variant 1

   Depositor

   Shamshad Cockcroft

   Article

   Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

   Insert

   RdgBbeta (PITPNC1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

8. Kv7.4 (1-645, Δ368-523)/HA tag/pcDNA3.1

   Plasmid

   #111459

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Ha Tag

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-523), C643A

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcs2-N-HRI(1-138)-GFP-IRES-mCherry

   Plasmid

   #226100

   Purpose

   HRI N-terminal region (residues 1-138) stability reporter construct for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   HRI (EIF2AK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains only N-HRI (aa 1-138)

   Promoter

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CEA-N domain residues 1-132 (CEACAM5)-pET30b

    Plasmid

    #174693

    Purpose

    Plasmid expressing CEA-N domain

    Depositor

    Jean Gariepy

    Article

    Abdul-Wahid et al Int J Cancer. 2016 Aug 15;139(4):841-53.

    Insert

    CEA-N Domain
    

    Use

    Tags

    TEV Cleavage Site and hexahistidine (HIS) tag

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 Promoter

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUPD2 Register 1/3 Tnos:YFP:5'UTR (GB1507)

    Plasmid

    #160583

    Purpose

    Part 1/3 for assembly of site-specific recombination based Registers. Composed of the reversed sequences of the TNos terminator,YFP CDS and 5' UTR of 35S promoter (TNos:YFP:5'UTR)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    YFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MMW3709 - human expression plasmid for SpCas9 Cluster 1 + Q926A

    Plasmid

    #101179

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 + Q926A variant: CMV-T7-hSpCas9-Cluster1+Q926A(N692A, M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1+Q926A(N692A/M694A/Q695A/H698A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/M694A/Q695A/H698A/Q926A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. HLH Bmal Pas 1-465-VenC F423R/V435R

    Plasmid

    #47371

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, F423R/V435R

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

14. CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid

    #171379

    Purpose

    Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized Cas13X.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV-Cas13Y.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid

    #171381

    Purpose

    Expression vector for encoding a human codon-optimized Cas13Y.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized Cas13Y.1
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)

    Plasmid

    #174637

    Purpose

    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro) (Kif1a Synthetic, Mouse)
    

    Use

    Tags

    mVenus-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB80-KIF1A(1-365)-VVD52C-mVenus-SSPB(micro)

    Plasmid

    #174636

    Purpose

    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVD52C-mVenus-SSPB(micro) (Kif1a Synthetic, Mouse)
    

    Use

    Tags

    mVenus-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5 FLAG 17xS/T->A, catalytic active lipin 1

    Plasmid

    #32007

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S106A, S150A, (S281A, T282A), S285A, S287A, S293A…

    Promoter

    CMV, SP6

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro)

    Plasmid

    #174630

    Purpose

    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVD52C-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
    

    Use

    Tags

    GFP-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pB80-KIF1A(1-365)-VVDfast-GFP-SSPB(micro)

    Plasmid

    #174631

    Purpose

    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
    

    Use

    Tags

    GFP-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge