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We narrowed to 11,938 results for: emb

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Showing: 4521 - 4540 of 11938 results

  5. pB-EF1a-FastFUCCI-IRES-3xnls-mTagBFP2 (JDW 1511)

    Plasmid
    #242599
    Purpose
    A PiggyBac expression vector containing the human EF1a promoter driving FastFUCCI to label cell cycle state followed by an IRES-3xnls-mTagBFP2.
    Depositor
    Joshua Wythe
    Publication
    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043
    Insert
    mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110) (EEF1A1 Human)
    Expression
    Mammalian
    Promoter
    human EF1a
    Available since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242599-plasmid-map-sequence-id-495380

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  6. pCAG-lox-rox-STOP-rox-lynScarlet-lox-Lyn-NeonGreen

    Plasmid
    #175259
    Purpose
    Dual plasmid labeling membrane target protein of different neuronal population with different fluorophore
    Depositor
    Frank Bradke, Sebastián Dupraz
    Publication
    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37
    Insert
    Lox-rox-STOP-rox-lyn-mScarlet-pA-lox
    Use
    Cre/Lox; Dre/rox
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    pCAG
    Available since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    175259-plasmid-map-sequence-id-375583

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  7. pUb-Cas9-EGFP_cU6:6-sgRNA

    Plasmid
    #190597
    Purpose
    The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.
    Depositor
    Claudia Rueckert
    Publication
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    EGFP - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190597-plasmid-map-sequence-id-379952

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  9. pAAV-EGFP.T2A.NCLX

    Plasmid
    #181873
    Purpose
    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter
    Depositor
    Hilmar Bading
    Publication
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Inserts
    solute carrier family 8 member B1 (Slc8a1 Mouse)
    EGFP
    Use
    AAV and Adenoviral
    Tags
    HA and myc
    Expression
    Mammalian
    Promoter
    synthetic hybrid CAG promoter and synthetic hybri…
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    181873-plasmid-map-sequence-id-355952

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  10. pcDNA3-AktAR-Kras

    Plasmid
    #123357
    Purpose
    FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.
    Depositor
    Jin Zhang
    Publication
    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AktAR-Kras
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    123357-plasmid-map-sequence-id-307467

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  11. pCEP4-HA-Flag-natT1R2 ECD-MHC

    Plasmid
    #113957
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class I
    Depositor
    Erik Procko
    Publication
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG, Signal/leader sequence from influenza hemag…
    Expression
    Mammalian
    Mutation
    residues 22-568 fused to a transmembrane tether
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113957-plasmid-map-sequence-id-215285

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  13. pCYC1pr-GNSI (LEU)

    Plasmid
    #111450
    Purpose
    GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.
    Depositor
    Alexey Merz
    Publication
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    111450-plasmid-map-sequence-id-211605

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  14. mCherry-FKBP-SAC1-HLx10

    Plasmid
    #108137
    Purpose
    Recruitable SAC1 with extended linker
    Depositor
    Gerry Hammond
    Publication
    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]10:SACM1L(521-587) (SACM1L Human)
    Expression
    Mammalian
    Mutation
    SACM1L(1-520):[EAAAR]10:SACM1L(521-587)
    Promoter
    CMV
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108137-plasmid-map-sequence-id-207728

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  17. pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

    Plasmid
    #80272
    Purpose
    mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted
    Depositor
    Andrew Holland
    Publication
    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Plk4 (PLK4 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of multi-phospho degron (amino acids 282…
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    80272-plasmid-map-sequence-id-151043

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  18. CmCitrine Lck w/ NosUTRs

    Plasmid
    #66785
    Purpose
    expression of fluorescent membranes in sea urchin primordial germ cells
    Depositor
    Amro Hamdoun
    Publication
    Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10
    Insert
    Lck (LCK Human)
    Use
    Sea urchin, zebrafish, xenopus
    Tags
    3' UTR from sea urchin Nanos, 5' UTR fr…
    Mutation
    Tandem repeat of LCK the N-terminal membrane targ…
    Promoter
    T7
    Available since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pSP64-hERG1a T623A

    Plasmid
    #53055
    Purpose
    Expresses alpha subunit of T623A hERG1a K channel in Xenopus oocytes
    Depositor
    Michael Sanguinetti
    Publication
    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    Use
    Xenopus oocyte expression
    Mutation
    changed Threonine 623 to Alanine
    Promoter
    SP6
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53055-plasmid-map-sequence-id-82520

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Showing: 4521 - 4540 of 11938 results