Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

167,910 results

Showing: 6811 - 6840 of 167910 results

  3. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid
    #181792
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181792-plasmid-map-sequence-id-358226

    Enlarge

  6. GPX4

    Plasmid
    #38797
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GPX4 (GPX4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    SeCys73 -> Gly
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38797-plasmid-map-sequence-id-428950

    Enlarge

  8. pET-BiFC

    Plasmid
    #87856
    Purpose
    pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187
    Inserts
    Encodes for MCS & C-term half of mVenus
    Encodes for MCS & N-term half of mVenus
    Tags
    BiFC C-terminal fragment
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87856-plasmid-map-sequence-id-175019

    Enlarge

  12. pGDP1 NDM-1

    Plasmid
    #112883
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    NDM-1 (blaNDM-1 Klebsiella pneumoniae)
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    Pbla
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112883-plasmid-map-sequence-id-219864

    Enlarge

  19. pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 (AAV1)

    Viral Prep
    #172922-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 (#172922). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 plasmid DNA. Syn-driven expression of calcium sensor jGCaMP8m in axons with cytosolic expression of mRuby3. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Promoter
    Syn
    Tags
    mRuby3
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only

  26. pPbU6_hdhfr/yfcu_Cas9

    Plasmid
    #216423
    Purpose
    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.
    Depositor
    Ellen Bushell
    Article
    Jonsdottir et al bioRxiv 2024.04.20.590404
    Type
    Empty backbone
    Use
    CRISPR; Expression in plasmodium berghei
    Expression
    Bacterial
    Available Since
    April 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216423-plasmid-map-sequence-id-431289

    Enlarge

  27. pcDNA3-TLR9-YFP

    Plasmid
    #13642
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 9 (TLR9 Human)
    Tags
    YFP
    Expression
    Mammalian
    Available Since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13642-plasmid-map-sequence-id-392328

    Enlarge

  28. pUSE-URA3-Sec7-msYFP2

    Plasmid
    #218969
    Purpose
    Gene replacement plasmid to label S. cerevisiae Sec7 with msYFP2
    Depositor
    Benjamin Glick
    Insert
    SEC7-msYFP2 (SEC7 Budding Yeast)
    Tags
    msYFP2
    Expression
    Yeast
    Available Since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218969-plasmid-map-sequence-id-445656

    Enlarge

  29. pAAV-hSyn-hChR2(H134R)-mCherry (AAV5)

    Viral Prep
    #26976-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-hChR2(H134R)-mCherry (#26976). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-mCherry plasmid DNA. Synapsin-driven, humanized channelrhodopsin H134R mutant, fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    mCherry
    Available Since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only

  30. pCMV-Neo-Bam

    Plasmid
    #16440
    Purpose
    Contains two independent transcription units--a CMV promoter/enhancer upstream of a MCS and a HSV thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.
    Depositor
    Bert Vogelstein
    Article
    Baker et al Science. 1990 Aug 24. 249(4971):912-5.
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    April 7, 2008
    Availability
    Academic Institutions and Nonprofits only
    16440-plasmid-map-sequence-id-211955

    Enlarge

Showing: 6811 - 6840 of 167910 results