163,297 results

1. pU6-Sp-pegRNA-RNF2_+5GtoT

   Plasmid

   #135957

   Purpose

   S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   RNF2_5GtoT pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-syn-flex-iGluSnFR4f-NGR-WPRE

   Plasmid

   #234442

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-NGR
   

   Use

   AAV and Cre/Lox

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. SP6-VEE-GFP

   Plasmid

   #58976

   Purpose

   expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Insert

   EGFP
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   26S subgenomic promoter

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-M4-AR

   Plasmid

   #171240

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor (hAR)

   Depositor

   Steven Balk

   Article

   Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

   Insert

   3xFlag-tagged human androgen receptor (AR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

   Plasmid

   #158967

   Purpose

   Ubiquitous expression of mtON in C. elegans

   Depositor

   Andrew Wojtovich

   Article

   Berry et al EMBO Rep. 2020 Apr 3;21(4):e49113. doi:

   Insert

   eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   eft-3

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-PKM2-R399E

   Plasmid

   #122691

   Purpose

   Express His-tagged PKM2 (R399E) protein in bacteria.

   Depositor

   Wen-Ching Wang

   Article

   Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

   Insert

   Pyruvate kinase M2 isoform (PKM Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   R399E

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. RfxCas13d-backbone

   Plasmid

   #165078

   Purpose

   Expression of RfxCas13d sgRNAs from a U6 promoter

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   Scaffold gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. plenti-CAG-gate-FLAG-IRES-GFP

   Plasmid

   #107398

   Purpose

   Lentiviral gateway destination vector

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB502

   Plasmid

   #74844

   Purpose

   Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBB550

    Plasmid

    #27396

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 . 7():32.

    Insert

    dnaK, dnaJ, GroESL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pN1/G3BP1-iRFP

    Plasmid

    #129339

    Purpose

    Stress granule marker

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    iRFP (from Dr. M. Matsuda)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

    Plasmid

    #199390

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    14xHis-Avi-SUMOEu

    Expression

    Bacterial

    Mutation

    Promoter

    T5-LacO

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCoofy51

    Plasmid

    #122005

    Purpose

    Baculovirus vector for parallel SLIC cloning containing N-terminal TwinStrep with PreScission Cleavage site

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    TwinStrep

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCoofy60

    Plasmid

    #122012

    Purpose

    Baculovirus vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site

    Depositor

    Peggy Stolt-Bergner, Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His6-eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLCHKO_intergenic-intergenic_2

    Plasmid

    #155078

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLCHKO_HPRT1_exon2_1_Lb

    Plasmid

    #155051

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-PKM2-H391Y

    Plasmid

    #122692

    Purpose

    Express His-tagged PKM2 (H391Y) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    H391Y

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-PRB

    Plasmid

    #89130

    Purpose

    mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418

    Depositor

    Elizabeth Wilson

    Article

    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

    Insert

    human progesterone receptor-B (PGR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. EcoFlex MoClo Kit

    Plasmid Kit

    #1000000080

    Purpose

    Golden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit design

    Depositor

    Paul Freemont

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pCAG-CreERT2

    Plasmid

    #14797

    Purpose

    Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBacPAK8-2xHA-MOF

    Plasmid

    #29763

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    MYST1 (KAT8 Human)
    

    Use

    Tags

    2xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pAC-SP6

    Plasmid

    #102418

    Purpose

    Ac transposase with SP6 promoter for in vitro transcription

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    Ac transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SP6

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1+ SMN1 myc HIS

    Plasmid

    #71687

    Purpose

    Mammalian expression of SMN1

    Depositor

    Gideon Dreyfuss

    Insert

    SMN1 (SMN1 Human)
    

    Use

    Tags

    HIS and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mEmerald-Alpha-V-Integrin-25

    Plasmid

    #53985

    Purpose

    Localization: Focal Adhesions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Alpha-V Integrin (ITGAV Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. psfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164477

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCnCre

    Plasmid

    #232907

    Purpose

    Electroporation vector for expression of Cre recombinase in Cuprividus necator

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

    Insert

    Cre recombinase (cre Synthetic)
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ptac-cymO

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pI3_EGFP

    Plasmid

    #239335

    Purpose

    Expresses the I3-01 nanocage subunit C-terminally tagged with EGFP in mammalian cells. Assembles into nanocages tagged with 60 EGFPs.

    Depositor

    Torsten Wittmann

    Insert

    I3-01
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pmStayGold_mStayGold_I3

    Plasmid

    #239339

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.

    Depositor

    Torsten Wittmann

    Insert

    I3-01
    

    Use

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available since

    June 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmScarlet3_I3

    Plasmid

    #239342

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.

    Depositor

    Torsten Wittmann

    Insert

    I3-01
    

    Use

    Tags

    mScarlet3

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. fabp10a:ER-CreER; cryaa:mCerulean

    Plasmid

    #230077

    Purpose

    Expression of ERCreER in zebrafish hepatocytes.

    Depositor

    Sumeet Pal Singh

    Article

    Hovhannisyan et al bioRxiv 2025.05.23.655316

    Insert

    ERT2-Cre-ERT2
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    fabp10a

    Available since

    May 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge