We narrowed to 9,991 results for: FIE

Showing: 6811 - 6840 of 9991 results

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pLentiGuide_GFP_NT135

Plasmid

#183118

Purpose

pLentiGuide modified to express GFP and a non-targeting sgRNA

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

Lentiviral

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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CHOP::GFP

Plasmid

#21898

Depositor

David Ron

Article

Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

Insert

CHOP (Ddit3 Mouse)

Use

Non-viral

Tags

EGFP

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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JH01

Plasmid

#73510

Purpose

GFPo_PH pCFJ150 - contains C. elegans sequence optimized GFP

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::GFPo::PH::tbb-2

Tags

GFP

Promoter

mex-5

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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JC201: pMVP/Ad/5-35-DEST

Plasmid

#121847

Purpose

pMVP destination vector, empty 5/35 fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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T7-RelA(S536E)

Plasmid

#24156

Depositor

Warner Greene

Article

Chen et al Mol Cell Biol. 2005 Sep . 25(18):7966-75

Insert

RelA (RELA Human)

Tags

T7 gp10

Expression

Mammalian

Mutation

S536E

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only
pCX-2A-2A

Plasmid

#74670

Purpose

based on pCX-C1 with F2A1/F2A2 inserted

Depositor

Roberto Giovannoni

Article

De Giorgi et al Plasmid. 2015 May;79:22-9. doi: 10.1016/

Type

Empty backbone

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSLHDR03_mCherry_tCD19_unique guide

Plasmid

#124062

Purpose

Construction of 1st donor for scarless genome editing with unique guide

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Inserts

mCherry
tCD19

Use

Mouse Targeting; Human targeting

Promoter

Human UbC modified

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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T7-RelA(KR)

Plasmid

#23251

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Tags

T7 gp10

Expression

Mammalian

Mutation

K218/221R, K310R

Available Since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
pCS2-LAMP1-mRFP

Plasmid

#169004

Purpose

Express rat LAMP1-mRFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Cell Rep. 2020 Dec 8;33(10):108477. doi:

Insert

rat LAMP1-mRFP

Use

Unspecified

Tags

mRFP

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFS478

Plasmid

#89066

Purpose

KanMX Z3EV promoter cassette

Depositor

Nick Rhind

Article

Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

Insert

Z3EV promoter cassette

Use

Yeast integration vector

Mutation

yeast Gal1 promoter modified with multiple Zif268…

Promoter

Z3EV promoter

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green

Plasmid

#170048

Purpose

Tri-Responsive Reporter Gene Construct for redgreen, triple red, and double green

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mScarlet
COmScarlet
mRuby3
mWasabi dN
mWasabi CO

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ccdb-DddA(G1397C)-UGI-Puro

Plasmid

#173230

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ccdb-DddA(G1397N)-UGI-Puro

Plasmid

#173231

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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OA984

Plasmid

#120363

Purpose

Expresses an single chain antibody 1C19 that targets all four DENV serotypes and expresses a red fluorescent protein reporter

Depositor

Omar Akbari

Article

Buchman et al PLoS Pathog. 2020 Jan 16;16(1):e1008103.

Insert

1C19 single chain antibody

Use

Unspecified

Promoter

AAEL010782 carboxypeptidase A (CPA) gene Promoter

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-memRFP

Plasmid

#224564

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

mRFP1

Use

Unspecified

Tags

Membrane localization signal

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMOD_C3001

Plasmid

#91094

Purpose

Module C, Promoter: 35S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

35S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSpyB-GFP

Plasmid

#220190

Purpose

sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG35 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG35

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDD283

Plasmid

#66824

Purpose

YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

YPET-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized YPET

Expression

Worm

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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T7-RelA(K310R)

Plasmid

#23250

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Tags

T7 gp10

Expression

Mammalian

Mutation

K310R

Available Since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
pMTTH+bromo5

Plasmid

#157803

Purpose

Expression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

bromo5

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hTXLNA-3xFLAG

Plasmid

#165471

Purpose

Human TXLNA mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

taxilin alpha (TXLNA Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB::Pleft* mCherry

Plasmid

#169411

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

pLeft
mCherry

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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Cherry-ICP0FxE-C3

Plasmid

#134562

Purpose

Expresses Cherry-ICP0 fusion protein with a RING finger deletion (FXE)

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

ICP0

Tags

Cherry

Expression

Mammalian

Mutation

RING finger deletion (FXE)

Promoter

CMV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB:Psmyc double blue

Plasmid

#170050

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blue

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mTagBFP2
CO BFP

Use

Unspecified

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET-MAT2A

Plasmid

#164820

Purpose

Expresses MAT2A in E. coli BL21(DE3)

Depositor

Vern Schramm

Article

Firestone et al J Am Chem Soc. 2017 Oct 4;139(39):13754-

Insert

Methionine Adenosyltransferase 2A (MAT2A Human)

Tags

6x His Tag

Expression

Bacterial

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) ACTB

Plasmid

#74705

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA

Use

CRISPR

Expression

Mammalian

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pSW454 - pUFO

Plasmid

#115978

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pUFO

Use

Unspecified

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-jREX-GECO1

Plasmid

#113941

Purpose

Bacterial expression of red fluorescent Ca2+ sensor jREX-GECO1

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jREX-GECO1

Tags

6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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JH02

Plasmid

#73511

Purpose

mCherryo_PH pCFJ150 - contians C. elegans sequence optimized mCherry

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::mCherryo::PH::tbb-2

Tags

mCherry

Promoter

pmex-5

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Plasmid L5 attB:Psmyc double green

Plasmid

#169412

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double green

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mWasabi
mWasabi CO

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge