We narrowed to 7,445 results for: ski

1. pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4-e2a-Myc

   Plasmid

   #193349

   Purpose

   tetO-S*KM (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4 + cMyc)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   TetO mini CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CD4-C-GFP HDRT Source (pTR 152)

   Plasmid

   #112018

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   CD4-GFP HDRT (CD4 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-EML4-ALK variant 3a I1171N

   Plasmid

   #183832

   Purpose

   Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N mutation in mammalian cells

   Depositor

   Aaron Hata

   Article

   Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-6a and ALK exon…

   Expression

   Mammalian

   Mutation

   I1171N

   Promoter

   CMV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-mEGFP-HMGB1-MUT-IDR2

   Plasmid

   #194552

   Purpose

   Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) IDR Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   HMGB1 (HMGB1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   K184Rfs*44

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRK5-mEGFP-HMGB1-MUT-Patchless

   Plasmid

   #194553

   Purpose

   Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "Patchless" variant

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   HMGB1 (HMGB1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   K184Rfs*44, truncation by stop codon after Lys209

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FLAG-D1-IRES-SS-D2-IRES-HA-D3

   Plasmid

   #215119

   Purpose

   Expresses FLAG-Cyclin D1, SS-Cyclin D2 and HA-Cyclin D3 in mammalian cells from an inducible lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Inserts

   CCND1 (CCND1 Human)
   CCND2 (CCND2 Human)
   CCND3 (CCND3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG-tag, HA-tag, and SS (STREP-STREP)

   Expression

   Mammalian

   Mutation

   Promoter

   IRES and TREG3

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-mEGFP-SQSTM1-MUT

   Plasmid

   #194583

   Purpose

   Mammalian Expression of mEGFP-SQSTM1 Mutant (V271Sfs*41) Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   SQSTM1 (SQSTM1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   V271Sfs*41

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pαH-S-GSAS-OMICRON

   Plasmid

   #180423

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

   Insert

   Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA

   Plasmid

   #184549

   Purpose

   Retroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tag

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Inserts

   Malate dehydogenase 1 (MDH1 Human)
   Malic Enzyme 1 (ME1 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG and HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CLTA-GFP HDRT Source (pTR 153)

    Plasmid

    #112016

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CLTA-GFP HDRT (CLTA Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUltra-MDH1-ME1

    Plasmid

    #184465

    Purpose

    Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Inserts

    Malate dehydrogenase 1 (MDH1 Human)
    Malic Enzyme 1 (ME1 Human)
    

    Use

    Lentiviral

    Tags

    fused to T2A

    Expression

    Mammalian

    Mutation

    deletion of stop codon

    Promoter

    UbC

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-mEGFP-FOXC1-MUT

    Plasmid

    #194567

    Purpose

    Mammalian Expression of mEGFP-FOXC1 Mutant (Q200Rfs*109) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    FOXC1 (FOXC1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Q200Rfs*109

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AiP5004 pAAV-Syn-DIO-TVA-dTom-SAD-B19G

    Plasmid

    #176286

    Purpose

    Cre-dependent AAV virus

    Depositor

    Shenqin Yao, The Allen Institute for Brain Science

    Article

    Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

    Insert

    TVA-dTomato-rabies Glycoprotein
    

    Use

    Tags

    dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AiP5005 pAAV-Syn-DIO-TVA66T-dTom-SAD-B19G

    Plasmid

    #176287

    Purpose

    Cre-dependent AAV virus

    Depositor

    Shenqin Yao, The Allen Institute for Brain Science

    Article

    Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

    Insert

    TVA66T-dTomato-rabies Glycoprotein
    

    Use

    Tags

    dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK5-mEGFP-HMGB1-delIDR

    Plasmid

    #194554

    Purpose

    Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein, "del IDR" variant

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    truncation by stop codon after Asn134

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPB-TetON-mEGFP-HMGB1-MUT

    Plasmid

    #194562

    Purpose

    Dox-inducible Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    K184Rfs*44

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5-mEGFP-HMGB1-MUT-Full-length

    Plasmid

    #194550

    Purpose

    Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    K184Rfs*44

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5-mEGFP-HMGB1-MUT-IDR

    Plasmid

    #194551

    Purpose

    Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) IDR Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    K184Rfs*44

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2

    Plasmid

    #210019

    Purpose

    tetO-OKS (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + Sox2)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Oct4-p2a-Klf4-IRES-Sox2 (Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO-mini-CMV

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CLTA-mCherry HDRT Source (pTR 177)

    Plasmid

    #112017

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CLTA-mCherry HDRT (CLTA Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-45b-mEGFP-HMGB1-K184Rfs

    Plasmid

    #194544

    Purpose

    Bacterial Expression of 6xHis mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Bacterial

    Mutation

    K184Rfs*44

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. BATF-GFP HDRT Source (pTR 146)

    Plasmid

    #112015

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    BATF-GFP HDRT (BATF Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5-mEGFP-HMGB1-MUT-RtoK

    Plasmid

    #194559

    Purpose

    Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R > K" variant

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    R > K substitutions after Lys185

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRK5-mEGFP-HMGB1-MUT-KtoR

    Plasmid

    #194560

    Purpose

    Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "K > R" variant

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    K > R substitutions after Lys185

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. RAB11A-BFP HDRT Source (pTR 145)

    Plasmid

    #112014

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-BFP HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRK5-mEGFP-RUNX1-MUT

    Plasmid

    #194575

    Purpose

    Mammalian Expression of mEGFP-RUNX1 Mutant (G363Afs*229) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    G363Afs*229

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. T7-VEE-CFP-e2a-SOX2AV-p2a-KLF4

    Plasmid

    #193359

    Purpose

    self-replicating RNA vector expressing CFP + human SOX2A61V + KLF4 + PuroR

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    CFP-e2a-SOX2AV-p2a-KLF4 (KLF4, SOX2 Human, Aequorea victoria)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    SOX2A61V is a highly cooperative point mutant of …

    Promoter

    T7

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRK5-mEGFP-FOXL2-MUT

    Plasmid

    #194585

    Purpose

    Mammalian Expression of mEGFP-FOXL2 Mutant (A328Pfs*28) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    FOXL2 (FOXL2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    A328Pfs*28

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPB-TetON-mEGFP-HMGB1-MUT-Patchless

    Plasmid

    #194563

    Purpose

    Dox-inducible Mammalian Expression of mEGFP-HMGB1 Mutant "Patchless" (K184Rfs*44, "Patchless" variant) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    K184Rfs*44, truncation by stop codon after Lys209

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CD4-C-BFP HDRT Source (pTR 176)

    Plasmid

    #112020

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-BFP HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge