We narrowed to 10,362 results for: Coli

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pZS157 CRISPEY RT/Cas9

Plasmid

#114454

Purpose

Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

Depositor

Hunter Fraser

Article

Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

Insert

SpCas9, Ec86-RT

Use

CRISPR

Tags

SV40-NLS

Expression

Yeast

Mutation

S. cerevisiae codon optimized

Promoter

GAL1-GAL10

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pNP2157

Plasmid

#222732

Purpose

pNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 Helper (WT)

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pST_116_LVL2 cam

Plasmid

#179332

Purpose

NT-CRISPR plasmid for a single gRNA.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout

Use

CRISPR

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPN-mKate (PT7LacO-PN-mKate)

Plasmid

#191126

Purpose

Expression of protein N tagged with mKate from a T7-lac inducible promoter

Depositor

Ariel Lindner

Article

Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

Insert

Protein N

Use

Synthetic Biology

Tags

mKate

Promoter

T7-lac inducible promoter

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDOC-GG

Plasmid

#149377

Purpose

Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

Depositor

Mark Pallen

Article

Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

Inserts

Fragment containing a kanamycin resistance cassette
Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
Fragment containing an I-SceI recognition sequence

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Tn7 helper plasmid

Plasmid

#141161

Purpose

Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

Depositor

Shimon Bershtein

Article

Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

Insert

Tn7 transposon machinery

Expression

Bacterial

Promoter

Arabinose-inducible pBAD

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp6-mNeongreen

Plasmid

#182388

Purpose

Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp6-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p-DsbC

Plasmid

#202485

Purpose

Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pOZITX-S1

Plasmid

#184925

Purpose

Expresses the catalytic S1 subunit of OZITX (EcPltAB)

Depositor

Jonathan Javitch

Article

Keen et al Commun Biol. 2022 Mar 23;5(1):256. doi:

Insert

codon optimized S1 subunit of OZITX

Expression

Mammalian

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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PT7lacO-His6-PheRSa-PheRSb

Plasmid

#224364

Purpose

Express PheRS protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

PheRS (pheS E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-eDHFR-MCS

Plasmid

#172108

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR

Depositor

Shinya Tsukiji

Article

Ishida et al J Am Chem Soc. 2013 Aug 28;135(34):12684

Insert

EGFP-eDHFR-20aa (folA E. coli)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSLS.492

Plasmid

#184957

Purpose

Test effect of a1/a2 length on editing rpoB using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22

Expression

Bacterial

Mutation

rpoB donor T1534C, a1/a2 length extended to 22 bp

Promoter

T7/lac

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSLS.402

Plasmid

#187202

Purpose

Expresses retron Eco1 RT

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Insert

Retron Eco1 Reverse Transcriptase

Use

Synthetic Biology

Expression

Bacterial

Promoter

mphR

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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eBFP2 (eBFP2)_CD

Plasmid

#66034

Purpose

MoClo Basic Part: CDS - Fluorescent protein. Blue. Derived from Addgene#14891. [C:eBFP2:D]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Fluorescent reporter - BFP

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBEST_OR2-OR1-Pr-UTR1_ClpP-T500

Plasmid

#153302

Purpose

Expresses ClpP protease in the cell-free TX-TL system

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

clpP (clpP Escherichia coli)

Expression

Bacterial

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFCIV-CEACAM6

Plasmid

#149303

Purpose

lentiviral packaging vector for CEACAM6 expression.

Depositor

James Fleckenstein

Article

Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

Insert

CEACAM6 (CEACAM6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

ubiquitin C

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBP-T7_RBS-His6-Thrombin

Plasmid

#72979

Purpose

T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

T7+RBS+His6+thrombin

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pNP568

Plasmid

#222725

Purpose

pNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cis

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 bridge RNA

Tags

sfGFP

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN YFP-DHFR(DD)

Plasmid

#29326

Depositor

Thomas Wandless

Article

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

YFP-DHFR(DD)

Use

Retroviral

Tags

HA and YFP

Mutation

Change Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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pET30a-AlkB-D135S

Plasmid

#79051

Purpose

Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

Depositor

Tao Pan

Article

Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

Insert

AlkB (alkB E.coli)

Tags

His tag

Expression

Bacterial

Mutation

A truncated AlkB with deletion of the amino (N)-t…

Promoter

T7

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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