We narrowed to 6,884 results for: GFP expression plasmids

1. pSLQ5079_pHR_PGK_sfGFP_CoV-F1

   Plasmid

   #155303

   Purpose

   SARS-CoV-2 fluorescent reporter 1

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   Superfolder GFP fused with SARS-CoV-F1 (ORF1ab Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-MCS-EGFP

   Plasmid

   #164092

   Purpose

   expression of EGFP-tagged proteins under CAG promoter

   Depositor

   Maik Hintze

   Insert

   EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. XLone-Puro NFIL3 P2A eGFP NLS

   Plasmid

   #140026

   Purpose

   Tunable and temporal expression control of NFIL3 and nuclear eGFP

   Depositor

   Xiaoping Bao

   Insert

   NFIL3 and enhanced Green Fluorescent Protein (NFIL3 Human, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. qTAG-C-moxGFP-Puro-LAMP1

   Plasmid

   #207788

   Purpose

   Donor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. qTAG-N-Puro-moxGFP-LMNB1

   Plasmid

   #207773

   Purpose

   Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. qTAG-N-Puro-moxGFP-TUBA1B

   Plasmid

   #207767

   Purpose

   Donor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Puro-moxGFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pCOTS-pyl-GFP(35TAG)

   Plasmid

   #92047

   Purpose

   This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.

   Depositor

   Lital Alfonta

   Article

   Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

   Inserts

   EGFP
   Pyrrolysyl tRNA(cua)
   Pyrrolysyl tRNA synthetase (methanosarcina mazei)
   

   Use

   Replicative expression plasmid for cyanobacteria …

   Tags

   Histag

   Expression

   Mutation

   Tyrosine in position 35 of the GFP was mutated f…

   Promoter

   LeuP (native S. elongatus promoter), PpsbII, and …

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. qTAG-N-Puro-moxGFP-GOLGA2

   Plasmid

   #207792

   Purpose

   Donor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   GOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. qTAG-C-moxGFP-Puro-MAPRE1

   Plasmid

   #207794

   Purpose

   Donor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   MAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. qTAG-C-moxGFP-Puro-H3C2

    Plasmid

    #207783

    Purpose

    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. qTAG-N-Blast-moxGFP-LMNB1

    Plasmid

    #207772

    Purpose

    Donor template for Blast-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Blast-moxGFP Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. qTAG-N-Blast-moxGFP-TUBA1B

    Plasmid

    #207766

    Purpose

    Donor template for Blast-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Blast-moxGFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pEGFP-hsRPA32-Ala10 (MSW#508)

    Plasmid

    #208073

    Purpose

    expression of GFP-tagged hsRPA2-Ala10 in human cells

    Depositor

    Marc Wold

    Insert

    RPA2 (RPA2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutations of 10 ser/thr residues in N-them domain…

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-hsRPA32-Asp8 (MSW#507)

    Plasmid

    #208072

    Purpose

    expression of GFP-tagged hsRPA2-Asp8 in human cells

    Depositor

    Marc Wold

    Insert

    RPA2 (RPA2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutations of 8 ser and thr residues in N-term dom…

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHUE-eGFP-Clu-beta-tail

    Plasmid

    #234891

    Purpose

    Expression of a His6-Ubiquitin-eGFP-Clu(204-227) fusion protein

    Depositor

    Franz-Ulrich Hartl

    Insert

    eGFP-Clu(204-227) (CLU Human)
    

    Use

    Tags

    His6-Ubiquitin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRBC-sfGFP-134/150TAG

    Plasmid

    #174077

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoter

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Use

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    D134TAG + N150TAG

    Promoter

    T7

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid

    #215010

    Purpose

    Express a Mitophagy reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    pSu9 (Presequence of Fo-ATPase subunit 9)
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid

    #215011

    Purpose

    Express a Mitophagy reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    pSu9 (Presequence of Fo-ATPase subunit 9)
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-hSYN-eGFP

    Plasmid

    #162512

    Purpose

    AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (hSYN) promoter

    Depositor

    Bart Nieuwenhuis

    Article

    Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSYN

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT2-CAG-fGFP

    Plasmid

    #108714

    Purpose

    Transposon donor plasmid for long-term expression of membrane targetted GFP

    Depositor

    Madeline Lancaster

    Article

    Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666.

    Insert

    EGFP
    

    Use

    Tags

    6xHis and farnesylation signal

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. KU701: pMVP (L5-L4) eGFP

    Plasmid

    #121707

    Purpose

    pMVP L5-L4 entry plasmid, contains eGFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eGFP fusion to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eGFP (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAc5.1B-EGFP-DmSmaug-del584-762-V5His6_C

    Plasmid

    #146006

    Purpose

    Insect Expression of DmSmaug-del584-762

    Depositor

    Elisa Izaurralde

    Insert

    DmSmaug-del584-762 (smg Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    7 silent mutations and a deletion of the AA 961-9…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TK4-EGFP

    Plasmid

    #239047

    Purpose

    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

    Depositor

    Marius Wernig

    Inserts

    EGFP
    puroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p1.2-GS-eGFP

    Plasmid

    #162771

    Purpose

    Fluorescent reporter for CHO expression studies

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

    Insert

    enhanced green fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

    Plasmid

    #51656

    Purpose

    hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange

    Depositor

    Adam Cohen

    Insert

    Quasar2-mOrange-Chr64-GFP
    

    Use

    Lentiviral

    Tags

    GFP and mOrange

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A V XE

    Plasmid

    #113843

    Purpose

    Histone H2B-superfolder GFP and tdTomato linked by T2A (V linker)

    Depositor

    Mario Capecchi

    Insert

    H2B-sfGFP-T2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A Q XE

    Plasmid

    #113842

    Purpose

    Histone H2B-superfolder GFP and tdTomato linked by T2A (Q linker)

    Depositor

    Mario Capecchi

    Insert

    H2B-sfGFP-T2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pICH-roGFP2-ORP1

    Plasmid

    #191677

    Purpose

    Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    redox(ro)GFP2-ORP1
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AICSDP-43: GJA1-mEGFP

    Plasmid

    #109120

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1

    Depositor

    Allen Institute for Cell Science

    Insert

    GJA1 Homology Arms with linker-mEGFP (GJA1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    May 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. AICSDP-47: CTNNB1-mEGFP

    Plasmid

    #109119

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human CTNNB1

    Depositor

    Allen Institute for Cell Science

    Insert

    CTNNB1 Homology Arms with mEGFP-linker (CTNNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    May 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. qTAG-C-moxGFP-Zeo

    Plasmid

    #207732

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. qTAG-N-Zeo-moxGFP

    Plasmid

    #207699

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-moxGFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. AICSDP-27:SLC25A17-mEGFP

    Plasmid

    #101784

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SLC25A17

    Depositor

    Allen Institute for Cell Science

    Insert

    SLC25A17 Homology Arms with linker-mEGFP (SLC25A17 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Oct. 31, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. AICSDP-50: NPM1-mEGFP

    Plasmid

    #109122

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human NPM1

    Depositor

    Allen Institute for Cell Science

    Insert

    NPM1 Homology Arms with linker-mEGFP (NPM1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    May 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. AICSDP-41: ATP2A2-mEGFP

    Plasmid

    #114405

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human ATP2A2

    Depositor

    Allen Institute for Cell Science

    Insert

    ATP2A2 Homology Arms with mEGFP-linker (ATP2A2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. AICSDP-63: ACTN2-mEGFP

    Plasmid

    #124607

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Insert

    ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-AcGFP-progerin

    Plasmid

    #86947

    Purpose

    Construct used to synthesize progerin mRNA. Please note the plasmid contains LMNA gene with nucleotide C1824T mutation.

    Depositor

    Alex Hewitt

    Insert

    AcGFP-progerin (LMNA Human)
    

    Use

    AAV

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    progerin sequence is LMNA gene with nucleotide C1…

    Promoter

    CMV and T7

    Available since

    June 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. qTAG-C-moxGFP-Puro

    Plasmid

    #207721

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. qTAG-N-Blast-moxGFP

    Plasmid

    #207677

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-moxGFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pLenti_CMV-EGFP-2A-mNeonGreen

    Plasmid

    #171599

    Purpose

    Green lentiviral vector plasmid for pseudovirus experiments

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    EGFP
    mNeonGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. qTAG-C-moxGFP-Blast

    Plasmid

    #207710

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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42. qTAG-N-Puro-moxGFP

    Plasmid

    #207688

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-moxGFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. AICSDP-83:EZH2-mEGFP

    Plasmid

    #164499

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human EZH2

    Depositor

    Allen Institute for Cell Science

    Insert

    EZH2 Homology Arms with mEGFP-linker (EZH2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

44. pHBS985 TDP43 RRM-GFP G348C

    Plasmid

    #107832

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G348C mutation in LLPS reporter

    Promoter

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pHBS996 TDP43 RRM-GFP A382T

    Plasmid

    #107816

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A382T mutation in LLPS reporter

    Promoter

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pHBS961 TDP43 RRM-GFP G295S

    Plasmid

    #107820

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G295S mutation in LLPS reporter

    Promoter

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pHBS959 TDP43 RRM-GFP G295F

    Plasmid

    #107818

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G295F mutation in LLPS reporter

    Promoter

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pHBS1281 TDP43 RRM-GFP G348R

    Plasmid

    #107833

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G348R mutation in LLPS reporter

    Promoter

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pHBS997 TDP43 RRM-GFP A382D

    Plasmid

    #107815

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A382D mutation in LLPS reporter

    Promoter

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pHBS993 TDP43 RRM-GFP A382F

    Plasmid

    #107814

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A382F mutation in LLPS reporter

    Promoter

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge