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pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA

Plasmid

#133342

Purpose

for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE3 (plus strand)

Plasmid

#91844

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0621)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE3 (plus strand) (CD69 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Missing 1 base from 13 base polyT tract beginning…

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pRK-flag-GFP 1-10 tetra Cys

Plasmid

#78589

Purpose

Expression flag-GFP 1-10 tetra Cys in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-GFP 1-10 tetra Cys

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

Plasmid

#133334

Purpose

for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE5 (plus strand)

Plasmid

#91841

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0618)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE5 (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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1201 pAdTrack FKHR H215R AAA

Plasmid

#9039

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHR H215R AAA (FOXO1 Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

H215R T24A S256A S319A

Available Since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only
pGL4.23-IL2RA CaRE3 (minus strand)

Plasmid

#91835

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0612)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE3 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs7893324 A>G, rs7090530 G>T, rs7090512 A&g…

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pNAS1B split mCherry N-MESVD/TRAP(RK)-C

Plasmid

#61967

Purpose

Duet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVD

Depositor

Lynne Regan

Article

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

N-mCherry-MESVD
TRAP(RK)-C-mCherry

Expression

Bacterial

Promoter

PBAD and PLtetO

Available Since

Feb. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE5 (minus strand)

Plasmid

#91836

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0613)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE5 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE2 (plus strand)

Plasmid

#91846

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0623)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE2 (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs6602425 T>G, T>C at chr10:6117603

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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(P79) OCN/Wnt 10b transgene

Plasmid

#25878

Depositor

Ormond MacDougald

Insert

Wnt10b (Wnt10b Mouse)

Use

Mouse Targeting

Tags

B-globin intron/poly A coding sequence and OCN pr…

Expression

Mammalian

Available Since

Oct. 21, 2010

Availability

Academic Institutions and Nonprofits only
pAc5.1B-lambdaN-HA-DmTral-LSm-V5His6_B

Plasmid

#145996

Purpose

Insect Expression of DmTral-LSm

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSm (tral Fly)

Expression

Insect

Mutation

one silent mutation A198C compared to the sequenc…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-LSm-V5His6_B

Plasmid

#145986

Purpose

Insect Expression of DmTral-LSm

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSm (tral Fly)

Expression

Insect

Mutation

one silent mutation A198C compared to the sequenc…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral_97-543-V5His6_D

Plasmid

#146128

Purpose

Insect Expression of DmTral_97-543

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_97-543 (tral Fly)

Expression

Insect

Mutation

two silent mutations A198C, A387G and a mutation …

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral_99-393-V5His6_D

Plasmid

#146137

Purpose

Insect Expression of DmTral_99-393

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_99-393 (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral_394-543-V5His6_D

Plasmid

#146138

Purpose

Insect Expression of DmTral_394-543

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_394-543 (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-DFDF-KFKF_C

Plasmid

#146038

Purpose

Insect Expression of DmTral-DFDF-KFKF

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmTral-DFDF-KFKF (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-del46-60_C

Plasmid

#146043

Purpose

Insect Expression of DmTral-del46-60

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-del46-60 (tral Fly)

Expression

Insect

Mutation

two silent mutations A198C, A387G and a mutation …

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral_394-543-V5His6_D

Plasmid

#146134

Purpose

Insect Expression of DmTral_394-543

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_394-543 (tral Fly)

Expression

Insect

Mutation

two silent mutations (nucleotides A198C, A387G) a…

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

Plasmid

#146039

Purpose

Insect Expression of DmTral-FDF-ADA

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmTral-FDF-ADA (tral Fly)

Expression

Insect

Mutation

FDF to ADA (407-409) mutation. Additionally, thre…

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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