167,066 results
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Plasmid#99709PurposeMammalian expression of HA-Nprl2DepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only
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AAV-DIO-TC66T-2A-EGFP-2A-oG
Plasmid#178429PurposeExpresses TVA(TC66T), EGFP, and optimized Rabies G (oG) protein in a FLEX cassetteDepositorInsertsTVA950 Glu66→Thr
Optimized G
EGFP
UseAAV and Cre/Lox; Adeno-associated virusTagsExpressionMutationPromoterhSynAvailable SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-GOLGA2
Plasmid#207791PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.DepositorInsertsgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available SinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBC-Pfv-Sapphire_PURO
Plasmid#203651PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a puromycin selection marker.DepositorArticleInsertPfV
UseLentiviralTagsSapphireExpressionMutationPromoterAvailable SinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
ABE7.10-F148A
Plasmid#132946PurposeGene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.DepositorInsertABE7.10(F148A)
UseCRISPR; Base editorTagsExpressionMammalianMutationABE7.10(F148A)PromoterAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)
Plasmid#179002PurposeSp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSRKBB-empty
Plasmid#59449PurposeContains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromotermodified lacAvailable SinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-MRE11-WT
Plasmid#82033PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV_NLS-dSaCas9-NLS-VPR
Plasmid#68495PurposeAAV vector containing nuclease null SaCas9 fused to VPRDepositorInsertdSaCas9
UseAAVTagsVPRExpressionMammalianMutationPromoterCMVAvailable SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV::TVA/tRFP
Plasmid#164486PurposeDual CMV promoter plasmid to express TVA and TurboRFPDepositorInsertsUseTagsExpressionMammalianMutationPromotersCMV and sCMV IE94Available SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe EGFR Del1
Plasmid#32062DepositorAvailable SinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLM494
Plasmid#100539PurposeCEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.DepositorInsertsGGPP synthase
phytoene desaturase
phytoene-beta carotene synthase
HMG CoA reductase
UseCre/Lox and Synthetic BiologyTagsExpressionYeastMutationPromoterPGK1, TDH3, TIP1, and ZEO1Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
ClCry4 Y319D
Plasmid#160649PurposeExpression of protein for various experiments including finding interaction partnersDepositorInsertClcry4 PHR Y319D
UseTagsHexa hisExpressionBacterialMutationPromoterAvailable SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12c1
Plasmid#120872PurposeExpresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.DepositorInsertCas12c1
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-UBF
Plasmid#17656DepositorAvailable SinceApril 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
hROSA26 CRISPR-pX330
Plasmid#105927PurposehROSA26 targeting CRISPR plasmidDepositorInserthROSA26 gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable SinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCytERM_mScarlet-i_N1
Plasmid#85068PurposeIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)DepositorInsertCytERM
UseTagsmScarlet-iExpressionMammalianMutationaa1-29PromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMTMVi-M
Plasmid#118757PurposeIntermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.DepositorInsertTobacco mosaic virus cDNA fragment with a cloning polylinker
UseIntermediate to build tobacco mosaic virus recomb…TagsExpressionMutationPromoterAvailable SinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-sRGECO (AAV8)
Viral Prep#137125-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-sRGECO (#137125). In addition to the viral particles, you will also receive purified pAAV-Ef1a-sRGECO plasmid DNA. EF1a-driven expression of cytosolic sRGECO calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas-CmR(+)
Plasmid#113633PurposeChloramphenicol resistant derivative of pCas plasmidDepositorInsertChloramphenicol resistance gene
UseCRISPRTagsExpressionMutationPromoterPcatAvailable SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-FOXL2
Plasmid#192914PurposeBarcoded piggybac transposon vector with Dox-inducible expression of FOXL2DepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR_CrPV_xb
Plasmid#11509DepositorInsertFirefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
UseLuciferaseTagsExpressionMutationPromoterAvailable SinceMarch 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSL3936 (pDonor(AAVS1)_Pse)
Plasmid#200903PurposeEncodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a SA-T2A-PuroR-T2A-eGFP cargo. Total transposon size = 2,024 bp.DepositorInsertMini Tn7016 (AAVS1 NGS)
UseTagsExpressionMammalianMutationPromoterAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-MBP-SCov2-nsp12
Plasmid#154759PurposeExpression of SARS-CoV-2 nsp12DepositorInsertSARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionInsectMutationPromoterPolyhedronAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)
Plasmid#139996PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGT8163 (AATG_ttLbCas12a-i_TTCG)
Plasmid#192453Purposeintronized ttLbCas12a for in planta expressionDepositorInserttemperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
UseCRISPR and Synthetic Biology; Moclo compatible le…TagsExpressionMutationtemperature-tolerant mutation (D156R)PromoterAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
PEmax_2star_pet21A
Plasmid#227675PurposeExpression plasmid for PEMAX** protein in bacteria.DepositorInsertPEmax_2_star
UseCRISPRTagsHis-tagExpressionBacterialMutationPromoterT7Available SinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
STING-V2
Plasmid#124264PurposeC-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartmentsDepositorAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWB517
Plasmid#74859PurposeGateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro-EGFP-FAK
Plasmid#38194DepositorInsertPTK2 protein tyrosine kinase 2 (Ptk2 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable SinceNov. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCBFD1/HBFD1Bad
Plasmid#53364PurposeWill convert beta-carotene into astaxanthin in E. coli when expression of the hbfd1 cDNA is induced with arabinose. Use with pAC-BETA or pAC-BETAipi.DepositorInsertcbfd1
UseTagsExpressionBacterialMutationPromoterlacAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
hCRISPRi-v2, Membrane Proteins (h6), top 5 sgRNAs/gene
Pooled Library#83976PurposeHuman CRISPRi Pooled Library targeting membrane proteinsDepositorAvailable SinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZH81 pET21b Rat1-His
Plasmid#231650PurposeBacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tagDepositorAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH82 pET28a-Rai1
Plasmid#231651PurposeBacterial expression vector expressing yeast S. cerevisiae rai1, no tagDepositorAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH23 Dual G-less containing ySSA1 polyA
Plasmid#231647Purposeyeast S.cerevisiae SSA1 polyA termination region inserted between the dual G-less cassetteDepositorInsertSSA1 polyA termination region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH20 Dual G-less containing yCYC1 polyA
Plasmid#231646Purposeyeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassetteDepositorInsertCYC1 polyA termination region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
YIp204-PADH1-AFB2
Plasmid#99531PurposeS. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)DepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR
Plasmid#171240PurposeMammalian expression of 3xFlag-tagged human androgen receptor (hAR)DepositorInsert3xFlag-tagged human androgen receptor (AR Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
sUPRa
Plasmid#223242PurposesUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levelsDepositorInsertsUPRa
UseAAVTagsExpressionMutationPromoterCustomAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2629
Plasmid#142966PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
JDS246
Plasmid#43861PurposeExpresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promotersDepositorInsertmammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
UseCRISPRTags3X FLAGExpressionMammalianMutationPromoterCMVAvailable SinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
PDIA5-bio-His
Plasmid#52072PurposeExpresses full-length Protein disulfide-isomerase A5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPDIA5 (PDIA5 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-BLM
Plasmid#110299PurposeMammalian expression of a EGFP-tagged full length Bloom's syndrome helicaseDepositorInsertBLM (Bloom's Syndrome) helicase (BLM Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
mPapaya-pBAD
Plasmid#54838PurposeLocalization: Protein Expression Vector , Excitation: 530, Emission: 541DepositorTypeEmpty backboneUseTags6xHis and mPapayaExpressionBacterialMutationPromoterAvailable SinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
sgRNA_expression_vector
Plasmid#210212PurposeAn empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.DepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterU6Available SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
NKP38 GFP (1009)
Plasmid#62021Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-jGCaMP8s-WPRE (AAV1)
Viral Prep#176758-AAV1PurposeReady-to-use AAV1 particles produced from AAV-EF1a-jGCaMP8s-WPRE (#176758). In addition to the viral particles, you will also receive purified AAV-EF1a-jGCaMP8s-WPRE plasmid DNA. EF1a-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBT/EFS-Cas9-PuroTk
Plasmid#170542PurposeExpresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinaseDepositorInsertPuroTK
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54
Plasmid#111727PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ54 (polyQ repeat)PromoterCMV and P10Available SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3TP-lux
Plasmid#11767DepositorInsert3TP
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable SinceAug. 4, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits