Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,467 results for: tre promoter

Clear keywords
Showing: 9361 - 9400 of 9467 results

  6. pGEX-UbcH7(C86K)-10aa-linker-delta 1-29 and 117-160 Bactrocera parkin(C463A)

    Plasmid
    #128383
    Purpose
    expresses protein UbcH7 (C86K) fused to Bactrocera parkin (C463A) with residues 1-29 and 117-160 deleted
    Depositor
    Kalle Gehring
    Article
    Sauve et al Nat Struct Mol Biol. 2018 Jul;25(7):623-
    Insert
    UbcH7 (UBE2L3 Human)
    Tags
    Bactrocera dorsalis parkin with mutation C463A an…
    Expression
    Bacterial
    Mutation
    mutation C86K in UbcH7 gene
    Promoter
    lac promotor
    Available Since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128383-plasmid-map-sequence-id-254044

    Enlarge

  7. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175570-plasmid-map-sequence-id-344992

    Enlarge

  9. pGL4.23 C5_15

    Plasmid
    #60322
    Purpose
    This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.
    Depositor
    Jorge Ferrer
    Article
    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1
    Insert
    Non active element (nearest TSS PHLDA1) (PHLDA1 Human)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Available Since
    Dec. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pGEX-UbcH7(C86K)-10aa-linker-delta 1-29, 117-160 and 412-496 Bactrocera parkin

    Plasmid
    #128384
    Purpose
    expresses protein UbcH7 (C86K) fused to Bactrocera parkin with residues 1-29, 117-160 and 412-496 deleted
    Depositor
    Kalle Gehring
    Article
    Sauve et al Nat Struct Mol Biol. 2018 Jul;25(7):623-
    Insert
    UbcH7 (UBE2L3 Human)
    Tags
    Bactrocera dorsalis parkin deleted residues 1 to …
    Expression
    Bacterial
    Mutation
    mutation C86K in UbcH7 gene
    Promoter
    lac promotor
    Available Since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128384-plasmid-map-sequence-id-254046

    Enlarge

  13. pHP138 Myc-TRF2-deltaB deltaM

    Plasmid
    #164255
    Purpose
    for generation of mammalian cells with inducible expression of dominant negative TRF2 for induction of telomere crisis used together with any PiggyBac transposase plasmid
    Depositor
    John Maciejowski
    Article
    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2
    Insert
    TRF2
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    deletion of amino acids-86 and 496-542
    Promoter
    Tetracycline inducible promotor
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    164255-plasmid-map-sequence-id-329101

    Enlarge

  14. N295A (EcN), N229A (EcQ) MetNIQ in pBAD

    Plasmid
    #118581
    Purpose
    N295A Mutation of MetN in Methionine ABC Transporter and N229A Mutation in Methionine Binding Protein (MetQ) for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 229 to Alanine (Ala) and…
    Promoter
    None and araBAD Promotoer
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118581-plasmid-map-sequence-id-228549

    Enlarge

  15. N295A (EcN) MetNIQ in pBAD

    Plasmid
    #118256
    Purpose
    N295A Mutation of MetN in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 295 to Alanine (Ala)
    Promoter
    None and araBAD Promotoer
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118256-plasmid-map-sequence-id-228530

    Enlarge

  16. pBIG2abc_hsHAUScomplex_FL

    Plasmid
    #202203
    Purpose
    Baculoviral transfer vector to co-express 8 subunits of human augmin complex
    Depositor
    Thomas Surrey
    Article
    Ustinova et al J Cell Sci. 2023 Jun 15;136(12):jcs26109
    Inserts
    HAUS1 (HAUS1 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS3 (HAUS3 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS2 (HAUS2 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS6 (HAUS6 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS7 (HAUS7 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS4 (HAUS4 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS5 (HAUS5 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS8 (HAUS8 Human, codon-optimized form for expression in Trichoplusia ni cells)
    Tags
    -mGFP-TEV-TwinStrep and His-tag (6x)
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    202203-plasmid-map-sequence-id-407808

    Enlarge

  20. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid
    #227275
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227275-plasmid-map-sequence-id-452039

    Enlarge

  23. pCMV-eVRQR-P2A-EGFP (KAC1028)

    Plasmid
    #242663
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    eVRQR mutations in SpCas9(S55R/D1135V/G1218R/R133…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242663-plasmid-map-sequence-id-489032

    Enlarge

  24. pCMV-SpCas9-VQR-P2A-EGFP (LRF199)

    Plasmid
    #242664
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VQR mutations in SpCas9(D1135V/R1335Q/T1337R)
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242664-plasmid-map-sequence-id-488971

    Enlarge

  25. qTAG-C-Solo-mStayGold-H3C2

    Plasmid
    #227334
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H3C2 Homology Arms flanking a mStayGold Tag (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    227334-plasmid-map-sequence-id-453638

    Enlarge

  26. qTAG-C-Solo-miRFP670nano3-H3C2

    Plasmid
    #227335
    Purpose
    Donor template for miRFP670nano3 insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H3C2 Homology Arms flanking a miRFP670nano3 Tag (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227335-plasmid-map-sequence-id-452142

    Enlarge

  30. pET28a_SYK

    Plasmid
    #224240
    Purpose
    Expression of human SYK Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    syk (SYK Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    224240-plasmid-map-sequence-id-459259

    Enlarge

  31. pET28a_PTK2b

    Plasmid
    #224237
    Purpose
    Expression of human PTK2b Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    ptk2b (PTK2B Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224237-plasmid-map-sequence-id-458420

    Enlarge

  32. pET11a_PTK2b_Kinase

    Plasmid
    #224242
    Purpose
    Expression of human PTK2b Kinase domain in E. coli
    Depositor
    Andrew Mesecar
    Insert
    ptk2b_kinase (PTK2B Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224242-plasmid-map-sequence-id-458378

    Enlarge

  37. pCMV-ABE8e-eVRQR-P2A-EGFP (HES1425)

    Plasmid
    #242657
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with neVRQR(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8e-SpCas9-VRQR(S55R)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA, eVRQR mutations in SpCas…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242657-plasmid-map-sequence-id-488990

    Enlarge

  38. C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)

    Plasmid
    #242660
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242660-plasmid-map-sequence-id-489092

    Enlarge

  39. N-term AAV-ABE8e-SpCas9(S55R) (CA21)

    Plasmid
    #242659
    Purpose
    CBh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-SpCas9(S55R) - to be used with eVRQR constructs
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCBh-BPNLS(SV40)-TadA8e-gs-SpCas9(D10A;S55R)-[N-term]-NpuN-BPNLS(SV40)-WPRE-bGH_PA
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    SpCas9(S55R)
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242659-plasmid-map-sequence-id-489035

    Enlarge

  40. pCMV-ABE8.8m-SpG-P2A-EGFP (BKS965)

    Plasmid
    #242652
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpG(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8.8m-SpG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.8 mutations in TadA, SpG mutations in SpCas9…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242652-plasmid-map-sequence-id-488985

    Enlarge

Showing: 9361 - 9400 of 9467 results