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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,643 results for: NSI

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Showing: 4681 - 4700 of 12643 results

  2. pYPQAT48

    Plasmid
    #223420
    Purpose
    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for dicot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-Mb2Cas12a-RVRR-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223420-plasmid-map-sequence-id-452225

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  3. SpyTag-sfGFP

    Plasmid
    #184226
    Purpose
    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpyTag-sfGFP
    Tags
    His6, SpyTag, and TEV protease cleavage site
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184226-plasmid-map-sequence-id-361605

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  5. pYPQAT42

    Plasmid
    #223414
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-ZmUbi-gRNA scaffold-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223414-plasmid-map-sequence-id-454172

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  19. pJV53-Cas12a

    Plasmid
    #158706
    Purpose
    CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    TetO
    Available Since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158706-plasmid-map-sequence-id-320865

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  20. pYPQAT37

    Plasmid
    #223409
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223409-plasmid-map-sequence-id-453514

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Showing: 4681 - 4700 of 12643 results