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pDGE336

Plasmid

#153245

Purpose

M4 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE341

Plasmid

#153250

Purpose

M7 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXIZ-turq-3xFLAG-TREX1

Plasmid

#164232

Purpose

retroviral plasmid for turq-3xFLAG-TREX1

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Retroviral

Tags

FLAG and mturquoise

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-EGFP-Ecadherin

Plasmid

#65290

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Lentiviral

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-Brn2

Plasmid

#32925

Purpose

Retroviral expression of mouse Brn2

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Brn2 (Pou3f2 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-Sico-Red

Plasmid

#84882

Purpose

Cre-inducible shRNA

Depositor

Eun Mi Hwang

Article

Jung et al Exp Neurobiol. 2016 Jun;25(3):120-9. doi

Insert

Sico-Red

Use

AAV

Tags

mCherry

Promoter

EF1a and CMV

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt4-V5

Plasmid

#35929

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt4 (WNT4 Human)

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation C (492) --> T

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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PEP115E

Plasmid

#97401

Purpose

binary construct of Golgi-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

Golgi target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PEP116E

Plasmid

#97402

Purpose

binary construct of plasma membrane-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

Plasma membrane target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIN-EF1a-mNeonGreen-P2A-Cas9

Plasmid

#189807

Purpose

Retroviral delivery of Cas9

Depositor

Ellen Rothenberg

Article

Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

Insert

Cas9

Use

Retroviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-jRex-GECO

Plasmid

#169259

Purpose

AAV transfer plasmid for neuronal expression of jRex-GECO.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

JRex-GECO

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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Ubc-megf10-GFP

Plasmid

#40207

Depositor

Joshua Sanes

Article

Kay et al Nature. 2012 Mar 11;483(7390):465-9. doi

Insert

Megf10 (Megf10 Mouse)

Tags

GFP fusion

Expression

Mammalian

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRSET FLII12Pglu-700uDelta6

Plasmid

#13568

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLIPglu F16A Y12S-ECFP-D13R, 6aa linker

Tags

Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in mglB

Available Since

Feb. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTUT3

Plasmid

#60040

Purpose

Mammalian expression of FLAG-tagged mouse TUT3

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

TUT3 (Papd5 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
Ga13_2-mTq2

Plasmid

#112933

Purpose

Human Galpha13 tagged with mTurquoise, inserted at residue 128

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

Galpha13

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pDR111_mannose_Bgal no NLS

Plasmid

#188399

Purpose

Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Tags

PhoD secretion peptide

Expression

Bacterial

Promoter

D-mannose

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJM005

Plasmid

#134150

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRS-puro shNEDD4L #1

Plasmid

#27016

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

shRNA NEDD4L #1 (NEDD4L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pT2ADWpuro_2paRAF

Plasmid

#129653

Purpose

For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pDGE655

Plasmid

#153253

Purpose

M1 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge